Mouse Anti-MX1 Recombinant Antibody (CBFYM-2849) (CBMAB-M3042-FY)

Name: Mouse Anti-MX1 Recombinant Antibody (CBFYM-2849)
SKU: CBMAB-M3042-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2849

Application

ICC, IHC-Fr, IHC-P, ELISA, WB

Immunogen

The antibody is a mouse monoclonal antibody raised against MX1

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% glycerol

Preservative

0.02% Sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MX Dynamin Like GTPase 1

Introduction

This gene encodes a guanosine triphosphate -metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

4599

UniProt ID

P20591

Alternative Names

MX Dynamin Like GTPase 1; Interferon-Regulated Resistance GTP-Binding Protein MxA; Interferon-Inducible Protein P78; Interferon-Induced Protein P78; Myxoma Resistance Protein 1; IFI-78K; Myxovirus (Influenza) Resistance 1, Homolog Of Murine (Interferon-Inducible Protein P78); Myxovirus (Influenza Virus) Resistance 1, Interferon-Inducible Protein P78 (Mouse)

Function

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.

Biological Process

Antiviral innate immune response Source: ARUK-UCL
Apoptotic process Source: ProtInc
Defense response Source: ProtInc
Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB
Interleukin-27-mediated signaling pathway Source: ARUK-UCL
Negative regulation of viral genome replication Source: UniProtKB
Response to type I interferon Source: UniProtKB
Response to virus Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cytoplasm
Endoplasmic reticulum
Endoplasmic reticulum membrane
Other locations
perinuclear region
Note: Binds preferentially to negatively charged phospholipids (PubMed:21900240). Colocalizes with CCHFV protein N in the perinuclear region (PubMed:15047845).
Isoform 2:
Nucleus
Cytoplasm
Note: Translocates into the nuclei of HSV-1 infected cells (PubMed:20603636).

PTM

ISGylated.

McKellar, J., Arnaud-Arnould, M., Chaloin, L., Tauziet, M., Arpin-André, C., Pourcelot, O., ... & Goujon, C. (2023). An evolutionarily conserved N-terminal leucine is essential for MX1 GTPase antiviral activity against different families of RNA viruses. Journal of Biological Chemistry, 299(1).

Bayrou, C., Van Laere, A. S., Dam Van, P., Moula, N., Garigliany, M. M., & Desmecht, D. (2023). Anti-Schmallenberg virus activities of type I/III interferons-induced Mx1 GTPases from different mammalian species. Viruses, 15(5), 1055.

McKellar, J., Arnaud-Arnould, M., Chaloin, L., Tauziet, M., Arpin-André, C., Pourcelot, O., ... & Goujon, C. (2022). The N-terminal domain of MX1 proteins is essential for their antiviral activity against different families of RNA viruses. bioRxiv, 2022-05.

Selvaramesh, A. S., Kumar, P., Mishra, C., Bhattacharya, T. K., Bhushan, B., Tiwari, A. K., ... & Sharma, A. (2020). Cloning, characterization and expression of GTPase effecter domain of chicken Mx1 gene. The Indian Journal of Animal Sciences, 90(12), 1602-1605.

Sehgal, P. B., Yuan, H., Scott, M. F., Deng, Y., Liang, F. X., & Mackiewicz, A. (2020). Murine GFP-Mx1 forms nuclear condensates and associates with cytoplasmic intermediate filaments: Novel antiviral activity against VSV. Journal of Biological Chemistry, 295(52), 18023-18035.

Dam Van, P., Desmecht, D., Garigliany, M. M., Bui Tran Anh, D., & Van Laere, A. S. (2019). Anti-influenza a virus activities of type I/III interferons-induced Mx1 GTPases from different mammalian species. Journal of Interferon & Cytokine Research, 39(5), 274-282.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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