Sign in or Register   Sign in or Register
  |  

Mouse Anti-NANOS3 Recombinant Antibody (4B11) (CBMAB-N1127-WJ)

This product is a Mouse antibody that recognizes NANOS3. The antibody 4B11 can be used for immunoassay techniques such as: ELISA, WB.
See all NANOS3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4B11
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
nanos homolog 3 (Drosophila)
Introduction
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Entrez Gene ID
342977
UniProt ID
P60323
Alternative Names
Nanos C2HC-Type Zinc Finger 3; NOS3; Nanos Homolog 3 (Drosophila); Nanos Homolog 3; ZC2HC12C; NANOS1L; NOS-3;
Function
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Biological Process
Germ cell development Source: UniProtKB
Negative regulation of apoptotic signaling pathway Source: Ensembl
Negative regulation of translation Source: BHF-UCL
Oogenesis Source: GO_Central
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: BHF-UCL
Regulation of cell cycle Source: UniProtKB
Regulation of translation Source: UniProtKB
Spermatogenesis Source: UniProtKB
Cellular Location
Cytoplasm
Nucleus
Other locations
Stress granule
P-body
Note: Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body.

Mueller, M. L., McNabb, B. R., Owen, J. R., Hennig, S. L., Ledesma, A. V., Angove, M. L., ... & Van Eenennaam, A. L. (2023). Germline ablation achieved via CRISPR/Cas9 targeting of NANOS3 in bovine zygotes. Frontiers in Genome Editing, 5.

Wang, J., Ren, J., Wang, Q., Li, C., Han, Z., Chen, T., ... & Hai, T. (2023). Nanos3 knockout pigs to model transplantation and reconstruction of the germline. Cell Proliferation, 56(5).

Ilaslan, E., Kwiatkowska, K., Smialek, M. J., Sajek, M. P., Lemanska, Z., Alla, M., ... & Kusz-Zamelczyk, K. (2022). Distinct roles of NANOS1 and NANOS3 in the cell cycle and NANOS3-PUM1-FOXM1 axis to control G2/M phase in a human primordial germ cell model. International Journal of Molecular Sciences, 23(12), 6592.

Kogasaka, Y., Murakami, S., Yamashita, S., Kimura, D., Furumoto, Y., Iguchi, K., & Sendai, Y. (2022). Generation of germ cell-deficient pigs by NANOS3 knockout. Journal of Reproduction and Development, 68(6), 361-368.

Wright, D., Kiso, M., & Saga, Y. (2021). Genetic and structural analysis of the in vivo functional redundancy between murine NANOS2 and NANOS3. Development, 148(1), dev191916.

Miura, K., Matoba, S., Hirose, M., & Ogura, A. (2021). Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3. Biology of Reproduction, 104(1), 223-233.

Zhang, F., Liu, R., Liu, C., Zhang, H., & Lu, Y. (2020). Nanos3, a cancer-germline gene, promotes cell proliferation, migration, chemoresistance, and invasion of human glioblastoma. Cancer Cell International, 20(1), 1-14.

Imai, A., Hagiwara, Y., Niimi, Y., Tokumoto, T., Saga, Y., & Suzuki, A. (2020). Mouse dead end1 acts with Nanos2 and Nanos3 to regulate testicular teratoma incidence. PLoS One, 15(4), e0232047.

Zhou, L., Wang, X., Liu, Q., Xu, S., Zhao, H., Han, M., ... & Li, J. (2019). Visualization of turbot (Scophthalmus maximus) primordial germ cells in vivo using fluorescent protein mediated by the 3′ untranslated region of nanos3 or vasa gene. Marine biotechnology, 21, 671-682.

Xiao, Q., Sun, Y., Liang, X., Zhang, L., Onxayvieng, K., Li, Z., & Li, D. (2019). Visualizing primordial germ cell migration in embryos of rice field eel (Monopterus albus) using fluorescent protein tagged 3′ untranslated regions of nanos3, dead end and vasa. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 235, 62-69.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-NANOS3 Recombinant Antibody (4B11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare