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Mouse Anti-NCL Recombinant Antibody (E5M7K) (CBMAB-CP1775-LY)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
E5M7K
Application
WB, IF (ICC)
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human nucleolin protein.
Specificity
Human, Monkey
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Nucleolin
Introduction
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human4691
Monkey720965
Dog608445
UniProt ID
HumanP19338
MonkeyH9H3Z4
Alternative Names
Nucleolin; Protein C23; Nsr1; C23;
Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Biological Process
Angiogenesis Source: UniProtKB
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to leukemia inhibitory factor Source: Ensembl
Negative regulation of translation Source: CAFA
Positive regulation of mRNA splicing, via spliceosome Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription of nucleolar large rRNA by RNA polymerase I Source: UniProtKB
Cellular Location
Nucleus
nucleolus
Cytoplasm
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
PTM
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
Symmetrically methylated by PRMT5.

Thongchot, S., Aksonnam, K., Thuwajit, P., Yenchitsomanus, P. T., & Thuwajit, C. (2023). Nucleolin‑based targeting strategies in cancer treatment: Focus on cancer immunotherapy. International Journal of Molecular Medicine, 52(3), 1-14.

Yangngam, S., Prasopsiri, J., Hatthakarnkul, P., Thongchot, S., Thuwajit, P., Yenchitsomanus, P. T., ... & Thuwajit, C. (2022). Cellular localization of nucleolin determines the prognosis in cancers: a meta-analysis. Journal of Molecular Medicine, 100(8), 1145-1157.

Firlej, V., Soyeux, P., Nourieh, M., Huet, E., Semprez, F., Allory, Y., ... & Destouches, D. (2022). Overexpression of nucleolin and associated genes in prostate cancer. International Journal of Molecular Sciences, 23(9), 4491.

Brignole, C., Bensa, V., Fonseca, N. A., Del Zotto, G., Bruno, S., Cruz, A. F., ... & Pastorino, F. (2021). Cell surface Nucleolin represents a novel cellular target for neuroblastoma therapy. Journal of Experimental & Clinical Cancer Research, 40(1), 180.

Lin, Q., Ma, X., Hu, S., Li, R., Wei, X., Han, B., ... & Pang, Y. (2021). Overexpression of nucleolin is a potential prognostic marker in endometrial carcinoma. Cancer Management and Research, 1955-1965.

Figueiredo, J., Miranda, A., Lopes-Nunes, J., Carvalho, J., Alexandre, D., Valente, S., ... & Cruz, C. (2021). Targeting nucleolin by RNA G-quadruplex-forming motif. Biochemical Pharmacology, 189, 114418.

Kim, J. H., Bae, C., Kim, M. J., Song, I. H., Ryu, J. H., Choi, J. H., ... & Kim, J. I. (2020). A novel nucleolin-binding peptide for Cancer Theranostics. Theranostics, 10(20), 9153.

Dong, D., Zhu, S., Miao, Q., Zhu, J., Tang, A., Qi, R., ... & Liu, G. (2020). Nucleolin (NCL) inhibits the growth of peste des petits ruminants virus. The Journal of General Virology, 101(1), 33.

Wang, F., Zhou, S., Qi, D., Xiang, S. H., Wong, E. T., Wang, X., ... & Wu, E. (2019). Nucleolin is a functional binding protein for salinomycin in neuroblastoma stem cells. Journal of the American Chemical Society, 141(8), 3613-3622.

Bian, W. X., Xie, Y., Wang, X. N., Xu, G. H., Fu, B. S., Li, S., ... & Zhang, X. L. (2019). Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. Nucleic Acids Research, 47(1), 56-68.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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