NCL

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Full Name

Nucleolin

Function

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Biological Process

Angiogenesis Source: UniProtKB
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to leukemia inhibitory factor Source: Ensembl
Negative regulation of translation Source: CAFA
Positive regulation of mRNA splicing, via spliceosome Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription of nucleolar large rRNA by RNA polymerase I Source: UniProtKB

Cellular Location

Nucleus
nucleolus
Cytoplasm
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

PTM

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
Symmetrically methylated by PRMT5.

Anti-NCL antibodies

Mouse Anti-NCL Recombinant Antibody (CBWJC-4240) (CBMAB-C5595WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG

Specificity: Human, Rat

Clone: CBWJC-4240

Application*: WB, IP, IF, E

Mouse Anti-NCL Recombinant Antibody (ZN004) (CBMAB-N1428-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human, Mouse

Clone: ZN004

Application*: E, F, IC, IF, WB

Rabbit Anti-NCL Recombinant Antibody (D4C7O) (CBMAB-N1424-WJ)

Datasheet

SDS

Target: NCL

Host: Rabbit

Specificity: Human, Mouse, Rat, Monkey

Clone: D4C7O

Application*: WB, IP, IF, F

Mouse Anti-NCL Recombinant Antibody (10C7) (CBMAB-N1422-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgM, κ

Specificity: Human

Clone: 10C7

Application*: WB, IH

Mouse Anti-NCL Recombinant Antibody (4E2) (CBMAB-N1419-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 4E2

Application*: F, IC, IH, WB

Mouse Anti-NCL Recombinant Antibody (CBWJN-0890) (CBMAB-N0178-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0890

Application*: WB, F, P, IF

Mouse Anti-NCL Recombinant Antibody (CBWJN-0745) (CBMAB-N0177-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse

Clone: CBWJN-0745

Application*: F, P, WB, IF

Mouse Anti-NCL Recombinant Antibody (CBWJN-0730) (CBMAB-N0176-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0730

Application*: WB, F, P, IF, MC

Rabbit Anti-NCL Recombinant Antibody (CBWJN-0954) (CBMAB-N0175-WJ)

Datasheet

SDS

Target: NCL

Host: Rabbit

Antibody Isotype: IgG

Specificity: Mouse, Rat, Human

Clone: CBWJN-0954

Application*: WB, P, IC

Mouse Anti-NCL Recombinant Antibody (CBWJN-0424) (CBMAB-N0174-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Dog, Human, Monkey

Clone: CBWJN-0424

Application*: E, IC, IP, WB

Mouse Anti-NCL Recombinant Antibody (CBWJN-0361) (CBMAB-N0173-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG

Specificity: Human

Clone: CBWJN-0361

Application*: WB, F, P, IF, MC

Mouse Anti-NCL Recombinant Antibody (CBWJN-0360) (CBMAB-N0172-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0360

Application*: WB, F, P, IF, MC

Mouse Anti-NCL Recombinant Antibody (130CT43.8.5) (CBMAB-N0171-WJ)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 130CT43.8.5

Application*: E, IF, IH, WB

Mouse Anti-NCL Recombinant Antibody (E5M7K) (CBMAB-CP1775-LY)

Datasheet

SDS

Target: NCL

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Monkey

Clone: E5M7K

Application*: WB, IF (IC)

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)