Mouse Anti-NDEL1 Recombinant Antibody (1G10) (CBMAB-N1540-WJ)

Name: Mouse Anti-NDEL1 Recombinant Antibody (1G10)
SKU: CBMAB-N1540-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1G10

Application

FC, WB

Specificity

Human, Dog, Rat, Monkey

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.32 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NudE Neurodevelopment Protein 1 Like 1

Introduction

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]

Entrez Gene ID

Human	81565
Monkey	721973
Rat	170845
Dog	489495

UniProt ID

Human	Q9GZM8
Monkey	F6T3W7
Rat	Q78PB6
Dog	A0A5F4C9S1

Alternative Names

NudE Neurodevelopment Protein 1 Like 1; Mitosin-Associated Protein 1; Protein Nudel; MITAP1; NUDEL; EOPA; NudE Nuclear Distribution Gene E Homolog (A. Nidulans)-Like 1; NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1;

Function

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).

Biological Process

Activation of GTPase activity Source: Ensembl
Cell migration Source: GO_Central
Central nervous system neuron axonogenesis Source: Ensembl
Centrosome localization Source: GO_Central
Cerebral cortex radially oriented cell migration Source: Ensembl
Chromosome segregation Source: UniProtKB
Establishment of chromosome localization Source: GO_Central
Establishment of mitotic spindle orientation Source: GO_Central
Inner cell mass cell proliferation Source: Ensembl
Insulin receptor signaling pathway Source: Ensembl
Lysosome localization Source: UniProtKB
Microtubule nucleation Source: GO_Central
Mitotic centrosome separation Source: GO_Central
Neurofilament cytoskeleton organization Source: Ensembl
Neuron migration Source: Ensembl
Neuron projection extension Source: Ensembl
Nuclear membrane disassembly Source: Ensembl
Positive regulation of axon extension Source: Ensembl
Positive regulation of axon regeneration Source: Ensembl
Positive regulation of GTPase activity Source: CACAO
Positive regulation of ruffle assembly Source: UniProtKB
Regulation of intracellular protein transport Source: CACAO
Regulation of neuron projection development Source: GO_Central
Retrograde axonal transport Source: Ensembl
Vesicle transport along microtubule Source: GO_Central

Cellular Location

Cytoskeleton
Centrosome
Spindle
Other locations
Kinetochore
Note: Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.

PTM

Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Palmitoylation at Cys-273 reduces affinity for dynein.

Tsai, M. H., Ke, H. C., Lin, W. C., Nian, F. S., Huang, C. W., Cheng, H. Y., ... & Tsai, J. W. (2024). Novel lissencephaly-associated NDEL1 variant reveals distinct roles of NDE1 and NDEL1 in nucleokinesis and human cortical malformations. Acta Neuropathologica, 147(1), 13.

Janczyk, P. Ł., Żyłkiewicz, E., De Hoyos, H., West, T., Matson, D. R., Choi, W. C., ... & Stukenberg, P. T. (2023). Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles. Molecular Biology of the Cell, 34(1), br1.

Christoff, R. R., Nani, J. V., Lessa, G., Rabello, T., Rossi, A. D., Krenn, V., ... & Hayashi, M. A. (2023). Assessing the role of Ndel1 oligopeptidase activity in congenital Zika syndrome: Potential predictor of congenital syndrome endophenotype and treatment response. Journal of Neurochemistry, 166(4), 763-776.

Garrott, S. R., Gillies, J. P., Siva, A., Little, S. R., Jbeily, R. E., & DeSantis, M. E. (2023). Ndel1 modulates dynein activation in two distinct ways. bioRxiv.

Garrott, S. R., Gillies, J. P., & DeSantis, M. E. (2022). Nde1 and Ndel1: Outstanding mysteries in dynein-mediated transport. Frontiers in Cell and Developmental Biology, 10, 871935.

Kim, Y. B., Hlavaty, D., Maycock, J., & Lechler, T. (2021). Roles for Ndel1 in keratin organization and desmosome function. Molecular Biology of the Cell, 32(20), ar2.

Li, W., Wang, S., He, H., Qin, J., Cheng, X., Zhao, H., ... & Jin, G. (2021). Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle. Stem Cell Research & Therapy, 12, 1-13.

Ye, J., Li, J., Ye, F., Zhang, Y., Zhang, M., & Wang, C. (2020). Mechanistic insights into the interactions of dynein regulator Ndel1 with neuronal ankyrins and implications in polarity maintenance. Proceedings of the National Academy of Sciences, 117(2), 1207-1215.

Woo, Y., Kim, S. J., Suh, B. K., Kwak, Y., Jung, H. J., Nhung, T. T. M., ... & Park, S. K. (2019). Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis. elife, 8, e50850.

Jiang, Y., Song, Y., Wang, R., Hu, T., Zhang, D., Wang, Z., ... & Han, S. (2019). NFAT1-mediated regulation of NDEL1 promotes growth and invasion of glioma stem-like cells. Cancer research, 79(10), 2593-2603.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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