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Mouse Anti-NIN Recombinant Antibody (CBWJN-0478) (CBMAB-N2467-WJ)

This product is a Mouse antibody that recognizes NIN. The antibody CBWJN-0478 can be used for immunoassay techniques such as: ELISA, IP, WB.
See all NIN antibodies

Summary

Host Animal
Mouse
Specificity
Cattle, Human
Clone
CBWJN-0478
Antibody Isotype
IgG2a
Application
ELISA, IP, WB

Basic Information

Specificity
Cattle, Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ninein
Introduction
This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human51199
Cattle510366
UniProt ID
HumanQ8N4C6
CattleA0A3Q1NL90
Alternative Names
Ninein; Glycogen Synthase Kinase 3 Beta-Interacting Protein; Ninein (GSK3B Interacting Protein); HNinein; Ninein Centrosomal Protein; GSK3B-Interacting Protein; KIAA1565; SCKL7;
Function
Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061).
May also act as a centrosome maturation factor (PubMed:11956314).
May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203).
Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203).
Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061).
Biological Process
Centriole-centriole cohesionManual Assertion Based On ExperimentIMP:GO_Central
Centrosome localizationManual Assertion Based On ExperimentIBA:GO_Central
Centrosome-templated microtubule nucleationManual Assertion Based On ExperimentIBA:GO_Central
Collateral sproutingIEA:Ensembl
Corpus callosum morphogenesisIEA:Ensembl
Corticospinal tract morphogenesisIEA:Ensembl
Microtubule anchoring at centrosomeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of axonogenesisIEA:Ensembl
Positive regulation of microtubule polymerizationIEA:Ensembl
Protein localizationManual Assertion Based On ExperimentIMP:GO_Central
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. Localizes to the subdistal appendage region of the centriole in a DCTN1-dependent manner.
Isoform 6
Cytoplasm
Seems to have a dominant-negative effect on localization of other isoforms, promoting their dissociation from the centrosome.
Involvement in disease
Seckel syndrome 7 (SCKL7):
A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
PTM
Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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