Mouse Anti-NIN Recombinant Antibody (CBWJN-0478) (CBMAB-N2467-WJ)

Name: Mouse Anti-NIN Recombinant Antibody (CBWJN-0478)
SKU: CBMAB-N2467-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0478

Application

ELISA, IP, WB

Specificity

Cattle, Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.1% sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ninein

Introduction

This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	51199
Cattle	510366

UniProt ID

Human	Q8N4C6
Cattle	A0A3Q1NL90

Alternative Names

Ninein; Glycogen Synthase Kinase 3 Beta-Interacting Protein; Ninein (GSK3B Interacting Protein); HNinein; Ninein Centrosomal Protein; GSK3B-Interacting Protein; KIAA1565; SCKL7;

Function

Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061).
May also act as a centrosome maturation factor (PubMed:11956314).
May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203).
Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203).
Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061).

Biological Process

Centriole-centriole cohesionManual Assertion Based On ExperimentIMP:GO_Central
Centrosome localizationManual Assertion Based On ExperimentIBA:GO_Central
Centrosome-templated microtubule nucleationManual Assertion Based On ExperimentIBA:GO_Central
Collateral sproutingIEA:Ensembl
Corpus callosum morphogenesisIEA:Ensembl
Corticospinal tract morphogenesisIEA:Ensembl
Microtubule anchoring at centrosomeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of axonogenesisIEA:Ensembl
Positive regulation of microtubule polymerizationIEA:Ensembl
Protein localizationManual Assertion Based On ExperimentIMP:GO_Central

Cellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. Localizes to the subdistal appendage region of the centriole in a DCTN1-dependent manner.
Isoform 6
Cytoplasm
Seems to have a dominant-negative effect on localization of other isoforms, promoting their dissociation from the centrosome.

Involvement in disease

Seckel syndrome 7 (SCKL7):
A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.

PTM

Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.

Tillery, M. M., Zheng, C., Zheng, Y., & Megraw, T. L. (2023). Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization. bioRxiv, 2023-06.

Gilbert, T., Barbier, M., Duployer, B., Dufrançais, O., Martet, L. E., Dalbard, E., ... & Merdes, A. (2023). Loss of ninein interferes with osteoclast formation and causes premature ossification. bioRxiv, 2023-11.

Sandron, A. (2022). The Role of Ninein in the Regulation of Microtubule Organization at the Centrosome (Master's thesis).

Cao, Z., Ge, S., Xu, Z., & Ma, Y. Q. (2021). β3-Endonexin interacts with ninein in vascular endothelial cells to promote angiogenesis. Biochemical and biophysical research communications, 566, 75-79.

Jurmain, J. L. (2020). Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice.

Rosen, J. N., Azevedo, M., Soffar, D. B., Boyko, V. P., Brendel, M. B., Schulman, V. K., & Baylies, M. K. (2019). The Drosophila Ninein homologue Bsg25D cooperates with Ensconsin in myonuclear positioning. Journal of Cell Biology, 218(2), 524-540.

Hayakawa-Yano, Y., & Yano, M. (2019). An RNA switch of a large exon of ninein is regulated by the neural stem cell specific-RNA binding protein, Qki5. International Journal of Molecular Sciences, 20(5), 1010.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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