Mouse Anti-NPAS4 Recombinant Antibody (A1391) (CBMAB-AP12723LY)

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Basic Information

Host Animal
Mouse
Clone
A1391
Application
ICC/IF, IHC, WB
Immunogen
Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Neuronal PAS Domain Protein 4
Introduction
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
Human266743
Mouse225872
Rat266734
UniProt ID
HumanQ8IUM7
MouseQ8BGD7
RatQ8CJH6
Alternative Names
Neuronal PAS Domain Protein 4; Class E Basic Helix-Loop-Helix Protein 79; HLH-PAS Transcription Factor NXF; PAS Domain-Containing Protein 10; Neuronal PAS4; BHLHe79;
Function
SMC-495S
Biological Process
Cell differentiationIEA:UniProtKB-KW
Cellular response to corticosterone stimulusIEA:Ensembl
Excitatory postsynaptic potentialISS:UniProtKB
Inhibitory postsynaptic potentialISS:UniProtKB
Inhibitory synapse assemblyISS:UniProtKB
LearningISS:UniProtKB
Long-term memoryISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of synaptic plasticityISS:UniProtKB
Regulation of synaptic transmission, GABAergicISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Short-term memoryISS:UniProtKB
Social behaviorISS:UniProtKB
Cellular Location
Nucleus
PTM
Ubiquitinated, leading to degradation by the proteosome.

Pollina, E. A., Gilliam, D. T., Landau, A. T., Lin, C., Pajarillo, N., Davis, C. P., ... & Greenberg, M. E. (2023). A NPAS4–NuA4 complex couples synaptic activity to DNA repair. Nature, 614(7949), 732-741.

Sun, X., Jing, L., Li, F., Zhang, M., Diao, X., Zhuang, J., ... & Wu, D. (2022). Structures of NPAS4-ARNT and NPAS4-ARNT2 heterodimers reveal new dimerization modalities in the bHLH-PAS transcription factor family. Proceedings of the National Academy of Sciences, 119(46), e2208804119.

Yang, J., Serrano, P., Yin, X., Sun, X., Lin, Y., & Chen, S. X. (2022). Functionally distinct NPAS4-expressing somatostatin interneuron ensembles critical for motor skill learning. Neuron, 110(20), 3339-3355.

Xu, P., Berto, S., Kulkarni, A., Jeong, B., Joseph, C., Cox, K. H., ... & Takahashi, J. S. (2021). NPAS4 regulates the transcriptional response of the suprachiasmatic nucleus to light and circadian behavior. Neuron, 109(20), 3268-3282.

Rossi, J. J., Rosenfeld, J. A., Chan, K. M., Streff, H., Nankivell, V., Peet, D. J., ... & Bersten, D. C. (2021). Molecular characterisation of rare loss-of-function NPAS3 and NPAS4 variants identified in individuals with neurodevelopmental disorders. Scientific reports, 11(1), 6602.

Kim, S., Park, D., Kim, J., Kim, D., Kim, H., Mori, T., ... & Ko, J. (2021). Npas4 regulates IQSEC3 expression in hippocampal somatostatin interneurons to mediate anxiety-like behavior. Cell Reports, 36(3).

Fu, J., Guo, O., Zhen, Z., & Zhen, J. (2020). Essential functions of the transcription factor Npas4 in neural circuit development, plasticity, and diseases. Frontiers in Neuroscience, 14, 603373.

Funahashi, Y., Ariza, A., Emi, R., Xu, Y., Shan, W., Suzuki, K., ... & Kaibuchi, K. (2019). Phosphorylation of Npas4 by MAPK regulates reward-related gene expression and behaviors. Cell reports, 29(10), 3235-3252.

Brigidi, G. S., Hayes, M. G., Santos, N. P. D., Hartzell, A. L., Texari, L., Lin, P. A., ... & Bloodgood, B. L. (2019). Genomic decoding of neuronal depolarization by stimulus-specific NPAS4 heterodimers. Cell, 179(2), 373-391.

Sharma, N., Pollina, E. A., Nagy, M. A., Yap, E. L., DiBiase, F. A., Hrvatin, S., ... & Greenberg, M. E. (2019). ARNT2 tunes activity-dependent gene expression through NCoR2-mediated repression and NPAS4-mediated activation. Neuron, 102(2), 390-406.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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