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Mouse Anti-NPAS4 Recombinant Antibody (S408-79) (CBMAB-N0484-WJ)

This product is a Mouse antibody that recognizes NPAS4. The antibody S408-79 can be used for immunoassay techniques such as: WB, ICC.
See all NPAS4 antibodies
Published Data
Fig.1 Protein levels of Npas4 in rats' lumbar spinal cord were analyzed by western blotting.
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Fig.2 Immunostain with Npas4 in the dorsal horn of spinal cord.
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Summary

Host Animal
Mouse
Specificity
Rat
Clone
S408-79
Antibody Isotype
IgG1
Application
WB, ICC

Basic Information

Specificity
Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% glycerol
Preservative
0.1% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Neuronal PAS Domain Protein 4
Introduction
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
266734
UniProt ID
Q8CJH6
Alternative Names
Neuronal PAS Domain Protein 4; Class E Basic Helix-Loop-Helix Protein 79; HLH-PAS Transcription Factor NXF; PAS Domain-Containing Protein 10; Neuronal PAS4; BHLHe79;
Function
Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus (By similarity).
Plays a key role in the structural and functional plasticity of neurons (By similarity).
Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (By similarity).
In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (By similarity).
In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (By similarity).
The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (By similarity).
Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (By similarity).
Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (PubMed:14701734).
Can activate the CME (CNS midline enhancer) element (PubMed:14701734).
Biological Process
Cell differentiationIEA:UniProtKB-KW
Cellular response to corticosterone stimulusIEA:Ensembl
Excitatory postsynaptic potentialISS:UniProtKB
Inhibitory postsynaptic potentialISS:UniProtKB
Inhibitory synapse assemblyISS:UniProtKB
LearningISS:UniProtKB
Long-term memoryISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of synaptic plasticityISS:UniProtKB
Regulation of synaptic transmission, GABAergicISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Short-term memoryISS:UniProtKB
Social behaviorISS:UniProtKB
Cellular Location
Nucleus
PTM
Ubiquitinated, leading to degradation by the proteosome.

Pollina, E. A., Gilliam, D. T., Landau, A. T., Lin, C., Pajarillo, N., Davis, C. P., ... & Greenberg, M. E. (2023). A NPAS4–NuA4 complex couples synaptic activity to DNA repair. Nature, 614(7949), 732-741.

Sun, X., Jing, L., Li, F., Zhang, M., Diao, X., Zhuang, J., ... & Wu, D. (2022). Structures of NPAS4-ARNT and NPAS4-ARNT2 heterodimers reveal new dimerization modalities in the bHLH-PAS transcription factor family. Proceedings of the National Academy of Sciences, 119(46), e2208804119.

Yang, J., Serrano, P., Yin, X., Sun, X., Lin, Y., & Chen, S. X. (2022). Functionally distinct NPAS4-expressing somatostatin interneuron ensembles critical for motor skill learning. Neuron, 110(20), 3339-3355.

Xu, P., Berto, S., Kulkarni, A., Jeong, B., Joseph, C., Cox, K. H., ... & Takahashi, J. S. (2021). NPAS4 regulates the transcriptional response of the suprachiasmatic nucleus to light and circadian behavior. Neuron, 109(20), 3268-3282.

Rossi, J. J., Rosenfeld, J. A., Chan, K. M., Streff, H., Nankivell, V., Peet, D. J., ... & Bersten, D. C. (2021). Molecular characterisation of rare loss-of-function NPAS3 and NPAS4 variants identified in individuals with neurodevelopmental disorders. Scientific reports, 11(1), 6602.

Kim, S., Park, D., Kim, J., Kim, D., Kim, H., Mori, T., ... & Ko, J. (2021). Npas4 regulates IQSEC3 expression in hippocampal somatostatin interneurons to mediate anxiety-like behavior. Cell Reports, 36(3).

Fu, J., Guo, O., Zhen, Z., & Zhen, J. (2020). Essential functions of the transcription factor Npas4 in neural circuit development, plasticity, and diseases. Frontiers in Neuroscience, 14, 603373.

Funahashi, Y., Ariza, A., Emi, R., Xu, Y., Shan, W., Suzuki, K., ... & Kaibuchi, K. (2019). Phosphorylation of Npas4 by MAPK regulates reward-related gene expression and behaviors. Cell reports, 29(10), 3235-3252.

Brigidi, G. S., Hayes, M. G., Santos, N. P. D., Hartzell, A. L., Texari, L., Lin, P. A., ... & Bloodgood, B. L. (2019). Genomic decoding of neuronal depolarization by stimulus-specific NPAS4 heterodimers. Cell, 179(2), 373-391.

Sharma, N., Pollina, E. A., Nagy, M. A., Yap, E. L., DiBiase, F. A., Hrvatin, S., ... & Greenberg, M. E. (2019). ARNT2 tunes activity-dependent gene expression through NCoR2-mediated repression and NPAS4-mediated activation. Neuron, 102(2), 390-406.

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For research use only. Not intended for any clinical use.

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