Mouse Anti-OPRM1 Recombinant Antibody (CBFYM-3040) (CBMAB-M3236-FY)

Mouse

Mouse

CBFYM-3040

IgG

WB

Mouse

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

1 mg/mL

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

OPIOID RECEPTOR MU 1

OPRM1 gene has been shown to confer functional differences to μ‐opioid receptors, such that the G variant binds β‐endorphin three times more strongly than the A variant.

mor; Oprm; muOR; MOP-R; MOR-1; M-OR-1; MOR-1O

Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone (PubMed:7905839, PubMed:7957926, PubMed:7891175, PubMed:12589820, PubMed:9689128).
Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors (PubMed:7905839).
The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15 (PubMed:12068084).
They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B. Also couples to adenylate cyclase stimulatory G alpha proteins. The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization. Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation. Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling whereas morphine induces only low desensitization and endocytosis. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. Involved in neurogenesis. Isoform 12 couples to GNAS and is proposed to be involved in excitatory effects (PubMed:20525224).
Isoform 16 and isoform 17 do not bind agonists but may act through oligomerization with binding-competent OPRM1 isoforms and reduce their ligand binding activity (PubMed:16580639).

Acute inflammatory response to antigenic stimulusIEA:Ensembl
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathwayISS:UniProtKB
Adenylate cyclase-inhibiting opioid receptor signaling pathwayIEA:Ensembl
Behavioral response to ethanolManual Assertion Based On ExperimentIMP:UniProtKB
Eating behaviorIEA:Ensembl
Estrous cycleIEA:Ensembl
Excitatory postsynaptic potentialIEA:Ensembl
G protein-coupled opioid receptor signaling pathwayISS:UniProtKB
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messengerManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathwayIEA:Ensembl
Negative regulation of cAMP-mediated signalingIDA:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of cytosolic calcium ion concentrationIDA:UniProtKB
Negative regulation of nitric oxide biosynthetic processIDA:UniProtKB
Negative regulation of Wnt protein secretionManual Assertion Based On ExperimentIMP:UniProtKB
Neuropeptide signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Phospholipase C-activating G protein-coupled receptor signaling pathwayISS:UniProtKB
Positive regulation of appetiteIEA:Ensembl
Positive regulation of cAMP-mediated signalingIDA:UniProtKB
Positive regulation of cytosolic calcium ion concentrationIDA:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeISS:UniProtKB
Positive regulation of neurogenesisISS:UniProtKB
Positive regulation of nitric oxide biosynthetic processIDA:UniProtKB
Regulation of cellular response to stressManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of NMDA receptor activityISS:UniProtKB
Regulation of sensory perception of painIEA:Ensembl
Response to cocaineIEA:Ensembl
Response to foodIEA:Ensembl
Response to growth factorIEA:Ensembl
Response to lipopolysaccharideIEA:Ensembl
Response to radiationIEA:Ensembl
Sensory perception1 PublicationNAS:UniProtKB
Sensory perception of painISS:UniProtKB
Wound healingIEA:Ensembl

Cell membrane
Cell projection, axon
Perikaryon
Cell projection, dendrite
Endosome
Is rapidly internalized after agonist binding.
Isoform 12
Cytoplasm

Extracellular: 1-68
Helical: 69-93
Cytoplasmic: 94-106
Helical: 107-131
Extracellular: 132-142
Helical: 143-165
Cytoplasmic: 166-185
Helical: 186-207
Extracellular: 208-230
Helical: 231-255
Cytoplasmic: 256-279
Helical: 280-306
Extracellular: 307-314
Helical: 315-338
Cytoplasmic: 339-400

Phosphorylated. Differentially phosphorylated in basal and agonist-induced conditions. Agonist-mediated phosphorylation modulates receptor internalization. Phosphorylated by GRK2 in a agonist-dependent manner. Phosphorylation at Tyr-168 requires receptor activation, is dependent on non-receptor protein tyrosine kinase Src and results in a decrease in agonist efficacy by reducing G-protein coupling efficiency. Phosphorylated on tyrosine residues; the phosphorylation is involved in agonist-induced G-protein-independent receptor down-regulation. Phosphorylation at Ser-377 is involved in G-protein-dependent but not beta-arrestin-dependent activation of the ERK pathway (By similarity).
Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.