Rabbit Anti-Phospho-MPL (Tyr626) Recombinant Antibody (D3H7B) (CBMAB-CP2836-LY)

Name: Rabbit Anti-Phospho-MPL (Tyr626) Recombinant Antibody (D3H7B)
SKU: CBMAB-CP2836-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

D3H7B

Application

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr626 of human TPOR protein.

Specificity

Human, Mouse, Rat, Monkey, Cattle

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MPL Proto-Oncogene, Thrombopoietin Receptor

Introduction

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	4352
Mouse	17480
Rat	366455
Monkey	723813
Cattle	528492

UniProt ID

Human	P40238
Mouse	Q08351
Rat	D4A2R0
Monkey	F7EPD2
Cattle	F1MX79

Alternative Names

MPL Proto-Oncogene; Thrombopoietin Receptor; Myeloproliferative Leukemia Virus Oncogene; Myeloproliferative Leukemia Protein; Proto-Oncogene C-Mpl; TPO-R; TPOR; Thrombopoietin Receptor;

Function

Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

Biological Process

Basophil homeostasis Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cytokine-mediated signaling pathway Source: GO_Central
Eosinophil homeostasis Source: Ensembl
Hemopoiesis Source: GO_Central
Monocyte homeostasis Source: Ensembl
Neutrophil homeostasis Source: Ensembl
Platelet aggregation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of lymphocyte proliferation Source: Ensembl
Positive regulation of platelet formation Source: Ensembl
Thrombopoietin-mediated signaling pathway Source: GO_Central

Cellular Location

Plasma membrane
Cell membrane
Golgi apparatus
Other locations
Cell surface

Involvement in disease

Congenital amegakaryocytic thrombocytopenia (CAMT):
Disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
Thrombocythemia 2 (THCYT2):
A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes.
Myelofibrosis with myeloid metaplasia (MMM):
A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly.

Topology

Extracellular: 26-491
Helical: 492-513
Cytoplasmic: 514-635

PTM

Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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