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Rabbit Anti-POLH Recombinant Antibody (E1I7T) (CBMAB-R0624-CN)

This product is a Rabbit antibody that recognizes POLH. The antibody E1I7T can be used for immunoassay techniques such as: WB.
See all POLH antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
E1I7T
Application
WB

Basic Information

Specificity
Human
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
DNA polymerase eta
Introduction
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
Entrez Gene ID
5429
UniProt ID
Q9Y253
Alternative Names
XPV; XP-V; RAD30; RAD30A
Function
DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906, PubMed:24553286, PubMed:16357261).
Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906).
Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006).
Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526).
Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940).
Targets POLI to replication foci (PubMed:12606586).
Biological Process
Cellular response to UV-CIEA:Ensembl
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
DNA replicationIEA:UniProtKB-KW
DNA synthesis involved in DNA repairManual Assertion Based On ExperimentIDA:UniProtKB
Error-free translesion synthesisTAS:Reactome
Error-prone translesion synthesisManual Assertion Based On ExperimentIBA:GO_Central
Pyrimidine dimer repairIEA:Ensembl
Regulation of DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Response to radiationManual Assertion Based On ExperimentIBA:GO_Central
Response to UV-CManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Binding to ubiquitinated PCNA mediates colocalization to replication foci during DNA replication and persists at sites of stalled replication forks following UV irradiation (PubMed:12606586, PubMed:16357261, PubMed:24553286).
After UV irradiation, recruited to DNA damage sites within 1 hour, to a maximum of about 80%; this recruitment may not be not restricted to cells active in DNA replication (PubMed:22801543).
Colocalizes with TRAIP to nuclear foci (PubMed:24553286).
Involvement in disease
Xeroderma pigmentosum variant type (XPV):
An autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most patients with the variant type of xeroderma pigmentosum do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.
PTM
Monoubiquitinated by RCHY1/PIRH2 (PubMed:20159558, PubMed:21791603).
Ubiquitination depends on integrity of the UBZ3-type zinc finger domain and is enhanced by TRAIP (PubMed:24553286, PubMed:16357261).
Ubiquitination inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions (PubMed:20159558, PubMed:21791603, PubMed:24553286).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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