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Mouse Anti-POLR1E Recombinant Antibody (C-11) (CBMAB-P2345-YC)

Provided herein is a Mouse monoclonal antibody against Human RNA Polymerase I Subunit E. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all POLR1E antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
C-11
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
RNA Polymerase I Subunit E
Introduction
POLR1E (RNA Polymerase I Subunit E) is a protein coding gene. Among its related pathways are RNA Polymerase I Promoter Escape and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. Gene Ontology annotations related to this gene include DNA-directed 5-3 RNA polymerase activity and RNA polymerase I transcription factor binding.
Entrez Gene ID
64425
UniProt ID
Q9GZS1
Alternative Names
PAF53; PRAF1
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors (PubMed:24207024).
Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (PubMed:24207024).
Biological Process
Nucleolar large rRNA transcription by RNA polymerase IManual Assertion Based On ExperimentIDA:UniProtKB
RNA polymerase I preinitiation complex assemblyManual Assertion Based On ExperimentIBA:GO_Central
Transcription elongation from RNA polymerase I promoterManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus, nucleolus
PTM
Acetylated at Lys-373 by CREBBP/CBP, leading to decreased RNA polymerase I transcription (PubMed:24207024).
In normal conditions, deacetylated by SIRT7, promoting the association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:24207024).
In response to stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased association of RNA polymerase I with the rDNA promoter region (PubMed:24207024).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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