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Rabbit Anti-PRMT1 Recombinant Antibody (E5A8F) (CBMAB-CP2076-LY)

The product is antibody recognizes PRMT1. The antibody E5A8F immunoassay techniques such as: WB.
See all PRMT1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
E5A8F
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu337 of human PRMT1 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PRMT1
Entrez Gene ID
Human3276
Mouse15469
Rat60421
Monkey719419
UniProt ID
HumanQ99873
MouseQ9JIF0
RatQ63009
MonkeyF6VMS3
Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP and MAP3K5/ASK1 (PubMed:10749851, PubMed:16879614, PubMed:26876602, PubMed:22095282, PubMed:26575292, PubMed:18951090, PubMed:25284789).
Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292).
Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (PubMed:18951090).
Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789).
Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed:22095282).
Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
Plays a role in regulating alternative splicing in the heart (By similarity).
Biological Process
Cardiac muscle tissue developmentISS:UniProtKB
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Histone H4-R3 methylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone methylationManual Assertion Based On ExperimentIDA:UniProtKB
In utero embryonic developmentIEA:Ensembl
Negative regulation of JNK cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of megakaryocyte differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Neuron projection developmentManual Assertion Based On ExperimentIMP:UniProtKB
Peptidyl-arginine methylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of erythrocyte differentiationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of hemoglobin biosynthetic processISS:BHF-UCL
Positive regulation of p38MAPK cascadeISS:BHF-UCL
Protein homooligomerizationManual Assertion Based On ExperimentIDA:UniProtKB
Protein methylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of megakaryocyte differentiationManual Assertion Based On ExperimentIDA:UniProtKB
RNA splicingISS:UniProtKB
Viral protein processingTAS:Reactome
Cellular Location
Nucleus
Nucleus, nucleoplasm
Cytoplasm
Cytoplasm, cytosol
Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity).
PTM
Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation (By similarity).
Ubiquitination is regulated by acetylation at Lys-228 and Lys-233 (By similarity).
Polyubiquitinated by E3 ubiquitin-protein ligase TRIM48, leading to suppression of MAP3K5/ASK1 methylation and subsequent MAP3K5 activation (PubMed:29186683).
Acetylation at Lys-228 and Lys-233 regulates ubiquitination by the SCF(FBXL17) complex. Acetylated at Lys-233 by p300/EP300. Deacetylated at Lys-228 and Lys-233 by SIRT1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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