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Rat Anti-RPS6KA4 (AA 1-772) Recombinant Antibody (CBFYM-3097) (CBMAB-M3293-FY)

This product is rat antibody that recognizes RPS6KA4. The antibody CBFYM-3097 can be used for immunoassay techniques such as: WB.
See all RPS6KA4 antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse
Clone
CBFYM-3097
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
E. coli-derived recombinant human MSK2, Met1-Ser772
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Concentration
LYOPH
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-772

Target

Full Name
RIBOSOMAL PROTEIN S6 KINASE A4
Introduction
This gene encodes a member of the RSK family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human8986
Mouse56613
UniProt ID
HumanO75676
MouseQ9Z2B9
Alternative Names
Ribosomal Protein S6 Kinase A4; Nuclear Mitogen- And Stress-Activated Protein Kinase 2; 90 KDa Ribosomal Protein S6 Kinase 4; Ribosomal Protein Kinase B; S6K-Alpha-4; EC 2.7.11.1; MSK2; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 4
Function
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
Biological Process
Biological Process histone phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process inflammatory responseIEA:UniProtKB-KW
Biological Process interleukin-1-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cytokine productionManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of CREB transcription factor activityManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process positive regulation of histone acetylationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of histone phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process post-translational protein modificationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
PTM
Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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