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Mouse Anti-RUNX3 Recombinant Antibody (527327) (CBMAB-R0373-CN)

This product is a Mouse antibody that recognizes RUNX3. The antibody 527327 can be used for immunoassay techniques such as: WB, FC, ICC, CyTOF.
See all RUNX3 antibodies
Published Data
Fig.1 Western blot analyses of RUNX1, RUNX3 and PKC-θ protein amounts in CUTLL1 cells after lentiviral overexpression of RUNX3 (LV-RUNX3).
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Fig.2 BM cells of the indicated genotypes were transduced with a RUNX3 encoding retrovirus (RV-Runx3).
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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
527327
Antibody Isotype
IgG2a
Application
WB, FC, ICC, CyTOF

Basic Information

Immunogen
E. coli-derived recombinant human RUNX3/CBFA3, Lys186-Tyr415, Accession # Q13761
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Concentration
LYOPH

Target

Full Name
Runt Related Transcription Factor 3
Introduction
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Entrez Gene ID
Human864
Mouse12399
UniProt ID
HumanQ13761
MouseQ64131
Alternative Names
Runt Related Transcription Factor 3; Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit; SL3/AKV Core-Binding Factor Alpha C Subunit; SL3-3 Enhancer Factor 1 Alpha C Subunit; Runt-Related Transcription Factor 3; Core-Binding Factor Subunit Alpha-3; Acute Myeloid Leukemia 2 Protein; Oncogene AML-2; CBF-Alpha-3; CBFA3; AML2;
Function
Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity).
May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712).
CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Biological Process
Biological Process chondrocyte differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process hemopoiesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of CD4-positive, alpha-beta T cell differentiationISS:UniProtKB
Biological Process negative regulation of cell cycleISS:UniProtKB
Biological Process negative regulation of epithelial cell proliferationISS:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Biological Process neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process ossificationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process peripheral nervous system neuron developmentManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process positive regulation of CD8-positive, alpha-beta T cell differentiationISS:UniProtKB
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of DNA-templated transcription1 PublicationNAS:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to transforming growth factor betaManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation.
PTM
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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