RUNX3

RUNX3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.

Full Name

Runt Related Transcription Factor 3

Function

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity).
May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712).
CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).

Biological Process

Biological Process chondrocyte differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process hemopoiesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of CD4-positive, alpha-beta T cell differentiationISS:UniProtKB
Biological Process negative regulation of cell cycleISS:UniProtKB
Biological Process negative regulation of epithelial cell proliferationISS:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Biological Process neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process ossificationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process peripheral nervous system neuron developmentManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process positive regulation of CD8-positive, alpha-beta T cell differentiationISS:UniProtKB
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of DNA-templated transcription1 PublicationNAS:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to transforming growth factor betaManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Cytoplasm
The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation.

PTM

Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.