Mouse Anti-TGFB1 Recombinant Antibody (CBYJT-2760) (CBMAB-T1979-YJ)

Mouse

Cattle, Chicken, Human, Mouse

CBYJT-2760

IgG1

FC

Bovine bone-derived TGFb1 and TGFb2

Cattle, Chicken, Human, Mouse

IgG1

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Saline, 0.5% BSA

0.09% Sodium Azide

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Transforming Growth Factor Beta 1

TGFB1 has several functions. Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (By similarity).

Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.
Latency-associated peptide
Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix (PubMed:28117447).
Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1 (PubMed:2022183, PubMed:8617200, PubMed:8939931, PubMed:19750484, PubMed:22278742, PubMed:19651619).
Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs) (PubMed:19750484, PubMed:22278742, PubMed:19651619).
Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447).
Transforming growth factor beta-1
Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity).
Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (PubMed:29109152).
At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus (PubMed:2022183, PubMed:8617200, PubMed:8939931, PubMed:19750484, PubMed:22278742, PubMed:19651619).
TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447).
Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed:20207738).
While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (By similarity).
Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity).
Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity).
At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity).
At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity).
Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401).
Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed:25893292, PubMed:29483653, PubMed:30696809).
Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed:25893292, PubMed:30696809).

Biological Process aortic valve morphogenesisSource:BHF-UCL1 Publication
Biological Process ATP biosynthetic processSource:BHF-UCL1 Publication
Biological Process BMP signaling pathwaySource:GO_Central1 Publication
Biological Process cell migrationSource:UniProtKB1 Publication
Biological Process cell-cell junction organizationSource:BHF-UCL1 Publication
Biological Process cellular response to organic cyclic compoundSource:UniProtKB1 Publication
Biological Process cellular response to transforming growth factor beta stimulusSource:BHF-UCL2 Publications
Biological Process chondrocyte differentiationSource:UniProtKB1 Publication
Biological Process common-partner SMAD protein phosphorylationSource:UniProtKB1 Publication
Biological Process connective tissue replacement involved in inflammatory response wound healingSource:BHF-UCL1 Publication
Biological Process embryonic liver developmentSource:BHF-UCL
Biological Process epidermal growth factor receptor signaling pathwaySource:BHF-UCL1 Publication
Biological Process epithelial to mesenchymal transitionSource:UniProtKB1 Publication
Biological Process extracellular matrix assemblySource:BHF-UCL1 Publication
Biological Process extrinsic apoptotic signaling pathwaySource:BHF-UCL1 Publication
Biological Process heart developmentSource:BHF-UCL
Biological Process heart valve morphogenesisSource:BHF-UCL
Biological Process hematopoietic progenitor cell differentiationSource:UniProtKB1 Publication
Biological Process hyaluronan catabolic processSource:UniProtKB1 Publication
Biological Process inflammatory responseSource:UniProtKB1 Publication
Biological Process lipopolysaccharide-mediated signaling pathwaySource:UniProtKB1 Publication
Biological Process lymph node developmentSource:UniProtKB
Biological Process macrophage derived foam cell differentiationSource:BHF-UCL1 Publication
Biological Process MAPK cascadeSource:UniProtKB1 Publication
Biological Process membrane protein intracellular domain proteolysisSource:UniProtKB1 Publication
Biological Process negative regulation of biomineral tissue developmentSource:BHF-UCL1 Publication
Biological Process negative regulation of blood vessel endothelial cell migrationSource:BHF-UCL1 Publication
Biological Process negative regulation of cell cycleSource:HGNC-UCL1 Publication
Biological Process negative regulation of cell differentiationSource:CACAO1 Publication
Biological Process negative regulation of cell growthSource:BHF-UCL1 Publication
Biological Process negative regulation of cell population proliferationSource:BHF-UCL2 Publications
Biological Process negative regulation of cell-cell adhesionSource:BHF-UCL1 Publication
Biological Process negative regulation of cytolysisSource:ARUK-UCL1 Publication
Biological Process negative regulation of DNA-templated transcriptionSource:UniProtKB1 Publication
Biological Process negative regulation of epithelial cell proliferationSource:BHF-UCL1 Publication
Biological Process negative regulation of extracellular matrix disassemblySource:BHF-UCL1 Publication
Biological Process negative regulation of fat cell differentiationSource:UniProtKB1 Publication
Biological Process negative regulation of gene expressionSource:BHF-UCL7 Publications
Biological Process negative regulation of hyaluronan biosynthetic processSource:UniProtKB1 Publication
Biological Process negative regulation of macrophage cytokine productionSource:DFLAT1 Publication
Biological Process negative regulation of miRNA maturationSource:BHF-UCL1 Publication
Biological Process negative regulation of miRNA transcriptionSource:ARUK-UCL1 Publication
Biological Process negative regulation of miRNA-mediated gene silencingSource:BHF-UCL1 Publication
Biological Process negative regulation of myoblast differentiationSource:UniProtKB1 Publication
Biological Process negative regulation of protein localization to plasma membraneSource:UniProtKB1 Publication
Biological Process negative regulation of protein phosphorylationSource:BHF-UCL1 Publication
Biological Process negative regulation of skeletal muscle tissue developmentSource:UniProtKB1 Publication
Biological Process neural tube closureSource:BHF-UCL
Biological Process neural tube developmentSource:BHF-UCL
Biological Process ossification involved in bone remodelingSource:BHF-UCL1 Publication
Biological Process pathway-restricted SMAD protein phosphorylationSource:UniProtKB1 Publication
Biological Process phosphate-containing compound metabolic processSource:BHF-UCL1 Publication
Biological Process positive regulation of blood vessel endothelial cell migrationSource:BHF-UCL1 Publication
Biological Process positive regulation of bone mineralizationSource:BHF-UCL1 Publication
Biological Process positive regulation of canonical Wnt signaling pathwaySource:CACAO2 Publications
Biological Process positive regulation of cardiac muscle cell differentiationSource:BHF-UCL1 Publication
Biological Process positive regulation of cell divisionSource:UniProtKB-KW
Biological Process positive regulation of cell migrationSource:BHF-UCL1 Publication
Biological Process positive regulation of cell population proliferationSource:BHF-UCL2 Publications
Biological Process positive regulation of chemotaxisSource:BHF-UCL1 Publication
Biological Process positive regulation of collagen biosynthetic processSource:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionSource:UniProtKB1 Publication
Biological Process positive regulation of epithelial to mesenchymal transitionSource:BHF-UCL2 Publications
Biological Process positive regulation of ERK1 and ERK2 cascadeSource:UniProtKB1 Publication
Biological Process positive regulation of extracellular matrix assemblySource:BHF-UCL1 Publication
Biological Process positive regulation of fibroblast migrationSource:BHF-UCL1 Publication
Biological Process positive regulation of gene expressionSource:UniProtKB1 Publication
Biological Process positive regulation of interleukin-17 productionSource:BHF-UCL1 Publication
Biological Process positive regulation of isotype switching to IgA isotypesSource:MGI1 Publication
Biological Process positive regulation of MAP kinase activitySource:BHF-UCL1 Publication
Biological Process positive regulation of microglia differentiationSource:UniProtKB
Biological Process positive regulation of miRNA maturationSource:BHF-UCL1 Publication
Biological Process positive regulation of miRNA transcriptionSource:BHF-UCL2 Publications
Biological Process positive regulation of NAD+ ADP-ribosyltransferase activitySource:BHF-UCL1 Publication
Biological Process positive regulation of pathway-restricted SMAD protein phosphorylationSource:BHF-UCL5 Publications
Biological Process positive regulation of peptidyl-serine phosphorylationSource:BHF-UCL2 Publications
Biological Process positive regulation of peptidyl-threonine phosphorylationSource:BHF-UCL2 Publications
Biological Process positive regulation of peptidyl-tyrosine phosphorylationSource:UniProtKB1 Publication
Biological Process positive regulation of phosphatidylinositol 3-kinase activitySource:BHF-UCL1 Publication
Biological Process positive regulation of protein dephosphorylationSource:BHF-UCL1 Publication
Biological Process positive regulation of protein import into nucleusSource:BHF-UCL1 Publication
Biological Process positive regulation of protein kinase B signalingSource:BHF-UCL1 Publication
Biological Process positive regulation of protein localization to nucleusSource:BHF-UCL1 Publication
Biological Process positive regulation of protein metabolic processSource:BHF-UCL1 Publication
Biological Process positive regulation of protein phosphorylationSource:UniProtKB1 Publication
Biological Process positive regulation of protein secretionSource:BHF-UCL1 Publication
Biological Process positive regulation of protein-containing complex assemblySource:BHF-UCL1 Publication
Biological Process positive regulation of receptor signaling pathway via STATSource:ARUK-UCL1 Publication
Biological Process positive regulation of SMAD protein signal transductionSource:BHF-UCL5 Publications
Biological Process positive regulation of smooth muscle cell proliferationSource:ARUK-UCL2 Publications
Biological Process positive regulation of superoxide anion generationSource:BHF-UCL1 Publication
Biological Process positive regulation of transcription by RNA polymerase IISource:UniProtKB1 Publication
Biological Process positive regulation of transcription regulatory region DNA bindingSource:BHF-UCL1 Publication
Biological Process positive regulation of vascular endothelial growth factor productionSource:BHF-UCL1 Publication
Biological Process positive regulation of vascular permeabilitySource:UniProtKB1 Publication
Biological Process protein export from nucleusSource:UniProtKB2 Publications
Biological Process protein kinase B signalingSource:UniProtKB1 Publication
Biological Process protein phosphorylationSource:ComplexPortal
Biological Process receptor catabolic processSource:BHF-UCL1 Publication
Biological Process regulation of bindingSource:UniProtKB
Biological Process regulation of blood vessel remodelingSource:BHF-UCL1 Publication
Biological Process regulation of cell migrationSource:BHF-UCL1 Publication
Biological Process regulation of cell population proliferationSource:GO_Central1 Publication
Biological Process regulation of DNA bindingSource:UniProtKB
Biological Process regulation of protein import into nucleusSource:UniProtKB
Biological Process regulation of SMAD protein signal transductionSource:UniProtKB1 Publication
Biological Process regulation of striated muscle tissue developmentSource:UniProtKB
Biological Process regulation of transforming growth factor beta receptor signaling pathwaySource:BHF-UCL1 Publication
Biological Process response to cholesterolSource:BHF-UCL1 Publication
Biological Process response to estradiolSource:BHF-UCL1 Publication
Biological Process response to progesteroneSource:BHF-UCL1 Publication
Biological Process response to woundingSource:BHF-UCL1 Publication
Biological Process salivary gland morphogenesisSource:BHF-UCL1 Publication
Biological Process SMAD protein complex assemblySource:BHF-UCL1 Publication
Biological Process SMAD protein signal transductionSource:GO_Central1 Publication
Biological Process transforming growth factor beta receptor signaling pathwaySource:BHF-UCL4 Publications
Biological Process transforming growth factor beta receptor signaling pathway involved in heart developmentSource:BHF-UCL
Biological Process vasculogenesisSource:BHF-UCL
Biological Process ventricular cardiac muscle tissue morphogenesisSource:BHF-UCL

Latency-associated peptide
Secreted, extracellular space, extracellular matrix
Transforming growth factor beta-1
Secreted

Camurati-Engelmann disease (CAEND):
An autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Inflammatory bowel disease, immunodeficiency, and encephalopathy (IBDIMDE):
An autosomal recessive disorder characterized by severe infantile inflammatory bowel disease manifesting as bloody diarrhea and failure to thrive, global developmental delay, epilepsy, brain atrophy and encephalopathy. Affected individuals suffer from recurrent infections associated with impaired T-cell response to stimulation and decreased T-cell subsets, including regulatory and helper T cells.

Transforming growth factor beta-1 proprotein: The precursor proprotein is cleaved in the Golgi apparatus by FURIN to form Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-1 inactive.
Latency-associated peptide
N-glycosylated (PubMed:3162913, PubMed:2493139, PubMed:28117447).
Deglycosylation leads to activation of Transforming growth factor beta-1 (TGF-beta-1); mechanisms triggering deglycosylation-driven activation of TGF-beta-1 are however unclear (PubMed:2493139).