Mouse Anti-TNF Recombinant Antibody (J2D10) (CBMAB-T2962-YJ)

Mouse

Human, Rat

J2D10

IgG1, κ

FC, IF, CyTOF

Recombinant human TNF-alpha

Human, Rat

IgG1, κ

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

PBS, pH 7.4

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

TNF Gene(Protein Coding) Tumor Necrosis Factor

TNF is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. TNF can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin; TNF-Alpha; TNFSF2; TNF-A; TNFA; Tumor Necrosis Factor (TNF Superfamily, Member 2); Tumor Necrosis Factor Ligand 1F

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208).
Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity).
Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Biological Process activation of cysteine-type endopeptidase activity involved in apoptotic processSource:UniProtKB1 Publication
Biological Process acute inflammatory responseSource:Ensembl
Biological Process astrocyte activationSource:ARUK-UCL1 Publication
Biological Process calcium-mediated signalingSource:Ensembl
Biological Process cellular response to amino acid stimulusSource:Ensembl
Biological Process cellular response to amyloid-betaSource:Ensembl
Biological Process cellular response to interferon-gammaSource:Ensembl
Biological Process cellular response to nicotineSource:UniProtKB1 Publication
Biological Process cellular response to organic cyclic compoundSource:UniProtKB1 Publication
Biological Process cellular response to retinoic acidSource:Ensembl
Biological Process cellular response to toxic substanceSource:Ensembl
Biological Process chronic inflammatory response to antigenic stimulusSource:BHF-UCL1 Publication
Biological Process circadian rhythmSource:Ensembl
Biological Process cognitionSource:ARUK-UCL1 Publication
Biological Process cortical actin cytoskeleton organizationSource:UniProtKB1 Publication
Biological Process defense response to Gram-positive bacteriumSource:Ensembl
Biological Process detection of mechanical stimulus involved in sensory perception of painSource:Ensembl
Biological Process embryonic digestive tract developmentSource:DFLAT1 Publication
Biological Process endothelial cell apoptotic processSource:Ensembl
Biological Process epithelial cell proliferation involved in salivary gland morphogenesisSource:Ensembl
Biological Process extracellular matrix organizationSource:Ensembl
Biological Process extrinsic apoptotic signaling pathwaySource:UniProtKB2 Publications
Biological Process extrinsic apoptotic signaling pathway via death domain receptorsSource:UniProtKB1 Publication
Biological Process glucose metabolic processSource:Ensembl
Biological Process humoral immune responseSource:Ensembl
Biological Process I-kappaB kinase/NF-kappaB signalingSource:Ensembl
Biological Process inflammatory responseSource:UniProtKB1 Publication
Biological Process intrinsic apoptotic signaling pathway in response to DNA damageSource:Ensembl
Biological Process JNK cascadeSource:Ensembl
Biological Process leukocyte migration involved in inflammatory responseSource:Ensembl
Biological Process leukocyte tethering or rollingSource:BHF-UCL1 Publication
Biological Process lipopolysaccharide-mediated signaling pathwaySource:UniProtKB1 Publication
Biological Process liver regenerationSource:Ensembl
Biological Process MAPK cascadeSource:UniProtKB1 Publication
Biological Process microglial cell activationSource:ARUK-UCL
Biological Process necroptotic signaling pathwaySource:UniProtKB3 Publications
Biological Process negative regulation of alkaline phosphatase activitySource:Ensembl
Biological Process negative regulation of amyloid-beta clearanceSource:ARUK-UCL
Biological Process negative regulation of apoptotic signaling pathwaySource:ARUK-UCL1 Publication
Biological Process negative regulation of bicellular tight junction assemblySource:UniProtKB1 Publication
Biological Process negative regulation of bile acid secretionSource:Ensembl
Biological Process negative regulation of blood vessel endothelial cell migrationSource:BHF-UCL1 Publication
Biological Process negative regulation of branching involved in lung morphogenesisSource:UniProtKB1 Publication
Biological Process negative regulation of cysteine-type endopeptidase activity involved in apoptotic processSource:ARUK-UCL1 Publication
Biological Process negative regulation of cytokine production involved in immune responseSource:BHF-UCL1 Publication
Biological Process negative regulation of DNA-templated transcriptionSource:UniProtKB2 Publications
Biological Process negative regulation of endothelial cell proliferationSource:Ensembl
Biological Process negative regulation of extrinsic apoptotic signaling pathway in absence of ligandSource:BHF-UCL2 Publications
Biological Process negative regulation of fat cell differentiationSource:BHF-UCL1 Publication
Biological Process negative regulation of gene expressionSource:UniProtKB1 Publication
Biological Process negative regulation of glucose importSource:Ensembl
Biological Process negative regulation of heart rateSource:Ensembl
Biological Process negative regulation of interleukin-6 productionSource:BHF-UCL1 Publication
Biological Process negative regulation of L-glutamate import across plasma membraneSource:Ensembl
Biological Process negative regulation of lipid catabolic processSource:BHF-UCL1 Publication
Biological Process negative regulation of lipid storageSource:BHF-UCL1 Publication
Biological Process negative regulation of miRNA maturationSource:ARUK-UCL2 Publications
Biological Process negative regulation of mitotic cell cycleSource:Ensembl
Biological Process negative regulation of myelinationSource:Ensembl
Biological Process negative regulation of myoblast differentiationSource:Ensembl
Biological Process negative regulation of myosin-light-chain-phosphatase activitySource:UniProtKB1 Publication
Biological Process negative regulation of neurogenesisSource:ARUK-UCL1 Publication
Biological Process negative regulation of osteoblast differentiationSource:Ensembl
Biological Process negative regulation of oxidative phosphorylationSource:Ensembl
Biological Process negative regulation of protein-containing complex disassemblySource:UniProtKB1 Publication
Biological Process negative regulation of signaling receptor activitySource:BHF-UCL1 Publication
Biological Process negative regulation of systemic arterial blood pressureSource:Ensembl
Biological Process negative regulation of transcription by RNA polymerase IISource:UniProtKB1 Publication
Biological Process negative regulation of vascular wound healingSource:BHF-UCL1 Publication
Biological Process negative regulation of viral genome replicationSource:BHF-UCL1 Publication
Biological Process osteoclast differentiationSource:Ensembl
Biological Process positive regulation of action potentialSource:Ensembl
Biological Process positive regulation of amyloid-beta formationSource:ARUK-UCL
Biological Process positive regulation of apoptotic processSource:UniProtKB3 Publications
Biological Process positive regulation of blood microparticle formationSource:BHF-UCL1 Publication
Biological Process positive regulation of calcidiol 1-monooxygenase activitySource:BHF-UCL1 Publication
Biological Process positive regulation of calcineurin-NFAT signaling cascadeSource:MGI1 Publication
Biological Process positive regulation of cell adhesionSource:UniProtKB1 Publication
Biological Process positive regulation of chemokine (C-X-C motif) ligand 2 productionSource:BHF-UCL1 Publication
Biological Process positive regulation of chemokine productionSource:BHF-UCL1 Publication
Biological Process positive regulation of chronic inflammatory response to antigenic stimulusSource:Ensembl
Biological Process positive regulation of cysteine-type endopeptidase activity involved in apoptotic processSource:UniProtKB1 Publication
Biological Process positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosisSource:Ensembl
Biological Process positive regulation of cytokine productionSource:BHF-UCL2 Publications
Biological Process positive regulation of cytokine production involved in inflammatory responseSource:ARUK-UCL1 Publication
Biological Process positive regulation of DNA biosynthetic processSource:Ensembl
Biological Process positive regulation of DNA-binding transcription factor activitySource:BHF-UCL1 Publication
Biological Process positive regulation of DNA-templated transcriptionSource:UniProtKB1 Publication
Biological Process positive regulation of ERK1 and ERK2 cascadeSource:BHF-UCL1 Publication
Biological Process positive regulation of extrinsic apoptotic signaling pathwaySource:GO_Central1 Publication
Biological Process positive regulation of fever generationSource:BHF-UCL
Biological Process positive regulation of fractalkine productionSource:ARUK-UCL
Biological Process positive regulation of gene expressionSource:UniProtKB2 Publications
Biological Process positive regulation of glial cell proliferationSource:ARUK-UCL1 Publication
Biological Process positive regulation of hair follicle developmentSource:Ensembl
Biological Process positive regulation of hepatocyte proliferationSource:Ensembl
Biological Process positive regulation of heterotypic cell-cell adhesionSource:BHF-UCL2 Publications
Biological Process positive regulation of humoral immune response mediated by circulating immunoglobulinSource:Ensembl
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signalingSource:BHF-UCL2 Publications
Biological Process positive regulation of I-kappaB phosphorylationSource:ARUK-UCL1 Publication
Biological Process positive regulation of inflammatory responseSource:ARUK-UCL1 Publication
Biological Process positive regulation of interferon-gamma productionSource:Ensembl
Biological Process positive regulation of interleukin-1 beta productionSource:ARUK-UCL1 Publication
Biological Process positive regulation of interleukin-18 productionSource:Ensembl
Biological Process positive regulation of interleukin-33 productionSource:ARUK-UCL1 Publication
Biological Process positive regulation of interleukin-6 productionSource:ARUK-UCL1 Publication
Biological Process positive regulation of interleukin-8 productionSource:BHF-UCL3 Publications
Biological Process positive regulation of JNK cascadeSource:Ensembl
Biological Process positive regulation of JUN kinase activitySource:UniProtKB1 Publication
Biological Process positive regulation of leukocyte adhesion to arterial endothelial cellSource:BHF-UCL1 Publication
Biological Process positive regulation of leukocyte adhesion to vascular endothelial cellSource:BHF-UCL1 Publication
Biological Process positive regulation of MAP kinase activitySource:UniProtKB1 Publication
Biological Process positive regulation of MAPK cascadeSource:BHF-UCL1 Publication
Biological Process positive regulation of membrane protein ectodomain proteolysisSource:BHF-UCL1 Publication
Biological Process positive regulation of miRNA transcriptionSource:BHF-UCL1 Publication
Biological Process positive regulation of mitotic nuclear divisionSource:Ensembl
Biological Process positive regulation of mononuclear cell migrationSource:BHF-UCL1 Publication
Biological Process positive regulation of neuroinflammatory responseSource:ARUK-UCL2 Publications
Biological Process positive regulation of neuron apoptotic processSource:ARUK-UCL
Biological Process positive regulation of neutrophil activationSource:Ensembl
Biological Process positive regulation of NF-kappaB transcription factor activitySource:UniProtKB3 Publications
Biological Process positive regulation of NIK/NF-kappaB signalingSource:UniProtKB1 Publication
Biological Process positive regulation of nitric oxide biosynthetic processSource:BHF-UCL1 Publication
Biological Process positive regulation of nitric-oxide synthase activitySource:ARUK-UCL1 Publication
Biological Process positive regulation of nitrogen compound metabolic processSource:ARUK-UCL
Biological Process positive regulation of osteoclast differentiationSource:BHF-UCL1 Publication
Biological Process positive regulation of oxidative stress-induced neuron deathSource:Ensembl
Biological Process positive regulation of peptidyl-serine phosphorylationSource:BHF-UCL2 Publications
Biological Process positive regulation of phagocytosisSource:AgBase1 Publication
Biological Process positive regulation of phosphatidylinositol 3-kinase signalingSource:ARUK-UCL
Biological Process positive regulation of podosome assemblySource:BHF-UCL1 Publication
Biological Process positive regulation of programmed cell deathSource:UniProtKB1 Publication
Biological Process positive regulation of protein catabolic processSource:AgBase1 Publication
Biological Process positive regulation of protein kinase activitySource:AgBase1 Publication
Biological Process positive regulation of protein kinase B signalingSource:ARUK-UCL
Biological Process positive regulation of protein localization to cell surfaceSource:BHF-UCL1 Publication
Biological Process positive regulation of protein localization to plasma membraneSource:ARUK-UCL
Biological Process positive regulation of protein phosphorylationSource:UniProtKB2 Publications
Biological Process positive regulation of protein transportSource:BHF-UCL1 Publication
Biological Process positive regulation of protein-containing complex assemblySource:BHF-UCL1 Publication
Biological Process positive regulation of protein-containing complex disassemblySource:UniProtKB1 Publication
Biological Process positive regulation of receptor signaling pathway via JAK-STATSource:ARUK-UCL
Biological Process positive regulation of smooth muscle cell proliferationSource:BHF-UCL1 Publication
Biological Process positive regulation of superoxide dismutase activitySource:BHF-UCL1 Publication
Biological Process positive regulation of synaptic transmissionSource:ARUK-UCL
Biological Process positive regulation of synoviocyte proliferationSource:ARUK-UCL1 Publication
Biological Process positive regulation of transcription by RNA polymerase IISource:UniProtKB1 Publication
Biological Process positive regulation of translational initiation by ironSource:Ensembl
Biological Process positive regulation of tyrosine phosphorylation of STAT proteinSource:ARUK-UCL
Biological Process positive regulation of vascular associated smooth muscle cell proliferationSource:Ensembl
Biological Process positive regulation of vitamin D biosynthetic processSource:BHF-UCL1 Publication
Biological Process protein kinase B signalingSource:UniProtKB1 Publication
Biological Process protein localization to plasma membraneSource:UniProtKB1 Publication
Biological Process regulation of branching involved in salivary gland morphogenesisSource:Ensembl
Biological Process regulation of endothelial cell apoptotic processSource:ARUK-UCL1 Publication
Biological Process regulation of establishment of endothelial barrierSource:UniProtKB1 Publication
Biological Process regulation of fat cell differentiationSource:ARUK-UCL
Biological Process regulation of I-kappaB kinase/NF-kappaB signalingSource:BHF-UCL1 Publication
Biological Process regulation of immunoglobulin productionSource:Ensembl
Biological Process regulation of insulin secretionSource:BHF-UCL1 Publication
Biological Process regulation of reactive oxygen species metabolic processSource:Ensembl
Biological Process regulation of synapse organizationSource:ARUK-UCL
Biological Process regulation of synaptic transmission, glutamatergicSource:ARUK-UCL1 Publication
Biological Process regulation of transcription by RNA polymerase IISource:ARUK-UCL
Biological Process response to 3,3',5-triiodo-L-thyronineSource:Ensembl
Biological Process response to activitySource:Ensembl
Biological Process response to ethanolSource:Ensembl
Biological Process response to fructoseSource:Ensembl
Biological Process response to glucocorticoidSource:BHF-UCL1 Publication
Biological Process response to gold nanoparticleSource:Ensembl
Biological Process response to Gram-negative bacteriumSource:Ensembl
Biological Process response to hypoxiaSource:Ensembl
Biological Process response to macrophage colony-stimulating factorSource:Ensembl
Biological Process response to nutrient levelsSource:Ensembl
Biological Process response to paracetamolSource:Ensembl
Biological Process response to radiationSource:Ensembl
Biological Process response to salt stressSource:BHF-UCL1 Publication
Biological Process response to virusSource:BHF-UCL1 Publication
Biological Process response to xenobiotic stimulusSource:Ensembl
Biological Process sequestering of triglycerideSource:BHF-UCL1 Publication
Biological Process skeletal muscle contractionSource:Ensembl
Biological Process toll-like receptor 3 signaling pathwaySource:Ensembl
Biological Process tumor necrosis factor-mediated signaling pathwaySource:ARUK-UCL2 Publications
Biological Process vascular endothelial growth factor productionSource:UniProtKB1 Publication
Biological Process vasodilationSource:Ensembl

Cell membrane
Tumor necrosis factor, membrane form
Membrane
Tumor necrosis factor, soluble form
Secreted
C-domain 1
Secreted
C-domain 2
Secreted

Psoriatic arthritis (PSORAS):
An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Cytoplasmic: 1-35
Helical: 36-56
Extracellular: 57-233

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Tumor necrosis factor, soluble form
The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.