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Mouse Anti-TNK2 Recombinant Antibody (ACK 1F4/9) (CBMAB-A4880-YC)

Provided herein is a Mouse monoclonal antibody against Drosophila Tyrosine Kinase Non Receptor 2. The antibody can be used for immunoassay techniques, such as WB, ELISA.
See all TNK2 antibodies

Summary

Host Animal
Mouse
Specificity
Drosophila
Clone
ACK 1F4/9
Antibody Isotype
IgM
Application
WB, ELISA

Basic Information

Immunogen
ACK peptides
Specificity
Drosophila
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TNK2 Gene(Protein Coding) Tyrosine Kinase Non Receptor 2
Introduction
TNK2 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an
Entrez Gene ID
10188
UniProt ID
Q07912
Alternative Names
Tyrosine Kinase Non Receptor 2; Tyrosine Kinase Non-Receptor Protein 2; Activated Cdc42-Associated Kinase 1; EC 2.7.10.2; ACK-1; ACK1; Tyrosine Kinase, Non-Receptor, 2;
Function
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP (PubMed:20110370).
Biological Process
Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:UniProtKB
EndocytosisIEA:UniProtKB-KW
Innate immune responseManual Assertion Based On ExperimentIBA:GO_Central
PhosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of peptidyl-tyrosine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of clathrin-dependent endocytosisManual Assertion Based On ExperimentIDA:UniProtKB
Small GTPase mediated signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Nucleus
Endosome
Cell junction, adherens junction
Cytoplasmic vesicle membrane
Cytoplasmic vesicle, clathrin-coated vesicle
Membrane, clathrin-coated pit
Cytoplasm, perinuclear region
Cytoplasm, cytosol
The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity).
Co-localizes with EGFR on endosomes (PubMed:20333297).
Nuclear translocation is CDC42-dependent (By similarity).
Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity).
PTM
Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits.
Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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