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Mouse Anti-TRAIP Recombinant Antibody (CBYJT-4535) (CBMAB-T4043-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TRAIP (TRAF Interacting Protein). The antibody can be used for immunoassay techniques, such as WB, FC.
See all TRAIP antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4535
Antibody Isotype
IgG1
Application
WB, FC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 67-313 of human TRAIP (NP_005870) produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.3, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 67-313

Target

Full Name
TRAF interacting protein
Introduction
TRAIP is a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.
Entrez Gene ID
10293
UniProt ID
Q9BWF2
Alternative Names
TRAF Interacting Protein; Ring Finger Protein 206; RING-Type E3 Ubiquitin Transferase TRAIP; RNF206; TRIP; E3 Ubiquitin-Protein Ligase TRAIP; TRAF-Interacting Protein; EC 2.3.2.27; SCKL9
Function
E3 ubiquitin ligase required to protect genome stability in response to replication stress (PubMed:25335891, PubMed:26781088, PubMed:27462463, PubMed:26711499, PubMed:26595769, PubMed:31545170).
Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription (By similarity).
Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex (By similarity).
Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3 (By similarity).
If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway (By similarity).
Only catalyzes ubiquitination of MCM7 when forks converge (By similarity).
Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: promotes ubiquitination of DPCs, leading to their degradation by the proteasome (By similarity).
Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity (PubMed:24553286).
The function in translesion synthesis is however controversial (PubMed:26595769).
Acts as a regulator of the spindle assembly checkpoint (PubMed:25335891).
Also acts as a negative regulator of innate immune signaling by inhibiting activation of NF-kappa-B mediated by TNF (PubMed:22945920).
Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation (PubMed:22945920).
Biological Process
Biological Process apoptotic process Source:GO_Central1 Publication
Biological Process cellular response to DNA damage stimulus Source:UniProtKB2 Publications
Biological Process negative regulation of interferon-beta production Source:Ensembl
Biological Process negative regulation of NF-kappaB transcription factor activity Source:Ensembl
Biological Process negative regulation of tumor necrosis factor-mediated signaling pathway Source:CACAO1 Publication
Biological Process protein ubiquitination Source:UniProtKB2 Publications
Biological Process protein-DNA covalent cross-linking repair Source:UniProtKB
Biological Process replication fork processing Source:UniProtKB1 Publication
Biological Process signal transduction Source:ProtInc1 Publication
Cellular Location
Nucleus, nucleoplasm
Nucleus, nucleolus
Chromosome
Cytoplasm
Cytoplasm, perinuclear region
In the nucleus, found in close proximity to PCNA, suggesting localization at replication foci (PubMed:26595769).
Localizes to DNA damage sites in response to replication stress (PubMed:26781088, PubMed:26595769, PubMed:26711499).
Involvement in disease
Seckel syndrome 9 (SCKL9):
A form of Seckel syndrome, a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and intellectual disability.
PTM
Sumoylated; sumoylation is required for nuclear localization (PubMed:26820530).
Sumoylation increases protein stability, possibly by preventing ubiquitination (PubMed:26820530).
Autoubiquitinated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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