Mouse Anti-TRPV4 Recombinant Antibody (4E11) (CBMAB-A9553-LY)

Mouse

Human

4E11

IgG2a, κ

sELISA, ELISA

TRPV4 (NP_067638.3, 772 a.a. ~ 871 a.a) partial recombinant protein with GST-pstS1 tag.

Human

IgG2a, κ

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

transient receptor potential cation channel, subfamily V, member 4

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

OTRPC4; TRP12; VR-OAC; VRL-2; VRL2; VROAC

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040, PubMed:29899501).
Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18826956, PubMed:18695040, PubMed:25256292, PubMed:20037586, PubMed:21964574).
Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956).
Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity).
Acts as a regulator of intracellular Ca2+ in synoviocytes (PubMed:19759329).
Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329).
Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040).
Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity).
Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (By similarity).
Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity).
Required for normal development and maintenance of bone and cartilage (PubMed:26249260).
In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (PubMed:29899501).
Isoform 5
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.
Isoform 2
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Isoform 4
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Isoform 6
Lacks channel activity, due to impaired oligomerization and intracellular retention.
(Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X.
(Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X.
(Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X.

Biological Process actin cytoskeleton reorganization Source:BHF-UCL
Biological Process actin filament organization Source:BHF-UCL
Biological Process blood vessel endothelial cell delamination Source:UniProtKB1 Publication
Biological Process calcium ion import Source:BHF-UCL
Biological Process calcium ion import across plasma membrane Source:GO_Central1 Publication
Biological Process calcium ion import into cytosol Source:UniProtKB1 Publication
Biological Process calcium ion transmembrane transport Source:UniProtKB1 Publication
Biological Process calcium ion transport Source:UniProtKB1 Publication
Biological Process cartilage development involved in endochondral bone morphogenesis Source:UniProtKB1 Publication
Biological Process cell volume homeostasis Source:UniProtKB1 Publication
Biological Process cell-cell junction assembly Source:UniProtKB
Biological Process cellular calcium ion homeostasis Source:UniProtKB1 Publication
Biological Process cellular hypotonic response Source:UniProtKB1 Publication
Biological Process cellular hypotonic salinity response Source:Ensembl
Biological Process cellular response to heat Source:UniProtKB
Biological Process cellular response to osmotic stress Source:UniProtKB
Biological Process cortical microtubule organization Source:BHF-UCL
Biological Process diet induced thermogenesis Source:Ensembl
Biological Process energy homeostasis Source:Ensembl
Biological Process glucose homeostasis Source:Ensembl
Biological Process hyperosmotic salinity response Source:Ensembl
Biological Process microtubule polymerization Source:BHF-UCL
Biological Process multicellular organismal water homeostasis Source:UniProtKB1 Publication
Biological Process negative regulation of brown fat cell differentiation Source:Ensembl
Biological Process negative regulation of neuron projection development Source:BHF-UCL
Biological Process negative regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process osmosensory signaling pathway Source:BHF-UCL
Biological Process positive regulation of chemokine (C-C motif) ligand 5 production Source:Ensembl
Biological Process positive regulation of chemokine (C-X-C motif) ligand 1 production Source:Ensembl
Biological Process positive regulation of cytosolic calcium ion concentration Source:UniProtKB1 Publication
Biological Process positive regulation of ERK1 and ERK2 cascade Source:Ensembl
Biological Process positive regulation of inflammatory response Source:Ensembl
Biological Process positive regulation of interleukin-6 production Source:Ensembl
Biological Process positive regulation of JNK cascade Source:Ensembl
Biological Process positive regulation of macrophage chemotaxis Source:Ensembl
Biological Process positive regulation of macrophage inflammatory protein 1 alpha production Source:Ensembl
Biological Process positive regulation of microtubule depolymerization Source:BHF-UCL
Biological Process positive regulation of monocyte chemotactic protein-1 production Source:Ensembl
Biological Process positive regulation of striated muscle contraction Source:Ensembl
Biological Process positive regulation of vascular permeability Source:UniProtKB1 Publication
Biological Process regulation of aerobic respiration Source:Ensembl
Biological Process regulation of response to osmotic stress Source:Ensembl
Biological Process response to insulin Source:Ensembl
Biological Process response to mechanical stimulus Source:UniProtKB1 Publication
Biological Process vasopressin secretion Source:Ensembl

Cell membrane
Apical cell membrane
Cell junction, adherens junction
Cell projection, cilium
Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
Isoform 1
Cell membrane
Isoform 5
Cell membrane
Isoform 2
Endoplasmic reticulum
Isoform 4
Endoplasmic reticulum
Isoform 6
Endoplasmic reticulum

Brachyolmia 3 (BCYM3):
A form of brachyolmia, a clinically and genetically heterogeneous skeletal dysplasia primarily affecting the spine and characterized by a short trunk, short stature, and platyspondyly. BCYM3 is an autosomal dominant form with severe scoliosis with or without kyphosis, and flattened irregular cervical vertebrae.
Spondylometaphyseal dysplasia Kozlowski type (SMDK):
A form of spondylometaphyseal dysplasia, a group of short stature disorders distinguished by abnormalities in the vertebrae and the metaphyses of the tubular bones. It is characterized by postnatal dwarfism, significant scoliosis and mild metaphyseal abnormalities in the pelvis. The vertebrae exhibit platyspondyly and overfaced pedicles.
Metatropic dysplasia (MTD):
A severe spondyloepimetaphyseal dysplasia characterized by short limbs with limitation and enlargement of joints and usually severe kyphoscoliosis. Radiologic features include severe platyspondyly, severe metaphyseal enlargement and shortening of long bones.
Neuronopathy, distal hereditary motor, 8 (HMN8):
A clinically variable, neuromuscular disorder characterized by congenital lower motor neuron disorder restricted to the lower part of the body. Clinical manifestations include non-progressive muscular atrophy, thigh muscle atrophy, weak thigh adductors, weak knee and foot extensors, minimal jaw muscle and neck flexor weakness, flexion contractures of knees and pes equinovarus. Tendon reflexes are normal.
Charcot-Marie-Tooth disease 2C (CMT2C):
An axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Scapuloperoneal spinal muscular atrophy (SPSMA):
A clinically variable neuromuscular disorder characterized by neurogenic scapuloperoneal amyotrophy, laryngeal palsy, congenital absence of muscles, progressive scapuloperoneal atrophy and progressive distal weakness and amyotrophy.
Spondyloepiphyseal dysplasia Maroteaux type (SEDM):
A clinically variable spondyloepiphyseal dysplasia with manifestations limited to the musculoskeletal system. Clinical features include short stature, brachydactyly, platyspondyly, short and stubby hands and feet, epiphyseal hypoplasia of the large joints, and iliac hypoplasia. Intelligence is normal.
Parastremmatic dwarfism (PSTD):
A bone dysplasia characterized by severe dwarfism, kyphoscoliosis, distortion and bowing of the extremities, and contractures of the large joints. Radiographically, the disease is characterized by a combination of decreased bone density, bowing of the long bones, platyspondyly and striking irregularities of endochondral ossification with areas of calcific stippling and streaking in radiolucent epiphyses, metaphyses and apophyses.
Digital arthropathy-brachydactyly, familial (FDAB):
A disorder characterized by irregularities in the proximal articular surfaces of the distal interphalangeal joints of the hand. Individuals appear normal at birth, with no clinical or radiographic evidence of a developmental skeletal dysplasia. The earliest changes appear during the first decade of life. By adulthood, all interphalangeal, metacarpophalangeal, and metatarsophalangeal joints are affected by a deforming, painful osteoarthritis. The remainder of the skeleton is clinically and radiographically unaffected.
Avascular necrosis of the femoral head, primary 2 (ANFH2):
A disease characterized by mechanical failure of the subchondral bone, and degeneration of the hip joint. It usually leads to destruction of the hip joint in the third to fifth decade of life. The clinical manifestations, such as pain on exertion, a limping gait, and a discrepancy in leg length, cause considerable disability.

Cytoplasmic: 1-469
Helical: 470-490
Extracellular: 491-507
Helical: 508-534
Cytoplasmic: 535-547
Helical: 548-568
Extracellular: 569-572
Helical: 573-593
Cytoplasmic: 594-608
Helical: 609-636
Extracellular: 637-665
Pore-forming: 666-685
Extracellular: 686-693
Helical: 694-722
Cytoplasmic: 723-871

N-glycosylated.