Mouse Anti-UBE2B Recombinant Antibody (CBFYH-2827) (CBMAB-H3895-FY)

Mouse

Human

CBFYH-2827

IgG2b

WB

Human

IgG2b

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

PBS, PH 7.3, 1% BSA, 50% glycerol

0.02% Sodium azide

1 mg/mL

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Ubiquitin Conjugating Enzyme E2 B; Ubiquitin-Conjugating Enzyme E2B (RAD6 Homolog); Ubiquitin-Conjugating Enzyme E2-17 KDa; E2 Ubiquitin-Conjugating Enzyme B; Ubiquitin Carrier Protein B; Ubiquitin-Protein Ligase B; RAD6 Homolog B; HHR6B; RAD6B; HR6B

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Biological Process apoptotic process Source:Ensembl
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process chiasma assembly Source:Ensembl
Biological Process DNA repair Source:UniProtKB1 Publication
Biological Process ectopic germ cell programmed cell death Source:Ensembl
Biological Process histone H2A ubiquitination Source:UniProtKB1 Publication
Biological Process histone lysine demethylation Source:Ensembl
Biological Process histone ubiquitination Source:GO_Central1 Publication
Biological Process in utero embryonic development Source:Ensembl
Biological Process meiotic telomere clustering Source:Ensembl
Biological Process negative regulation of apoptotic process Source:Ensembl
Biological Process negative regulation of cAMP-mediated signaling Source:UniProtKB1 Publication
Biological Process negative regulation of developmental process Source:Ensembl
Biological Process negative regulation of post-translational protein modification Source:Ensembl
Biological Process negative regulation of reproductive process Source:Ensembl
Biological Process positive regulation of canonical Wnt signaling pathway Source:UniProtKB
Biological Process positive regulation of reciprocal meiotic recombination Source:Ensembl
Biological Process postreplication repair Source:UniProtKB1 Publication
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process protein autoubiquitination Source:UniProtKB1 Publication
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:UniProtKB1 Publication
Biological Process protein stabilization Source:UniProtKB1 Publication
Biological Process protein ubiquitination Source:UniProtKB2 Publications
Biological Process regulation of histone modification Source:Ensembl
Biological Process response to UV Source:UniProtKB1 Publication
Biological Process response to xenobiotic stimulus Source:UniProtKB1 Publication
Biological Process sperm axoneme assembly Source:Ensembl
Biological Process spermatogenesis Source:UniProtKB1 Publication
Biological Process synaptonemal complex organization Source:Ensembl
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication

Cell membrane
Nucleus
In peripheral neurons, expressed both at the plasma membrane and in nuclei.