Mouse Anti-ULK3 Recombinant Antibody (CBT3307) (V2LY-0625-LY1926)

Name: Mouse Anti-ULK3 Recombinant Antibody (CBT3307)
SKU: V2LY-0625-LY1926
Rating: 5 (1 reviews)

See all ULK3 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-ULK3 Recombinant Antibody (CBT3307) (V2LY-0625-LY1926) in WB

Mouse Anti-ULK3 Recombinant Antibody (CBT3307) (V2LY-0625-LY1926) in IF

Mouse Anti-ULK3 Recombinant Antibody (CBT3307) (V2LY-0625-LY1926) in FC

Mouse Anti-ULK3 Recombinant Antibody (CBT3307) (V2LY-0625-LY1926) in IHC

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3307

Application

WB, IHC, ICC, FC

Immunogen

Purified recombinant fragment of human ULK3 expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:50-1:200
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Unc-51 Like Kinase 3

Entrez Gene ID

25989

Function

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Biological Process

Biological Process autophagosome organization Source:GO_Central1 Publication
Biological Process autophagy Source:UniProtKB1 Publication
Biological Process cellular senescence Source:UniProtKB1 Publication
Biological Process fibroblast activation Source:CACAO1 Publication
Biological Process negative regulation of smoothened signaling pathway Source:UniProtKB1 Publication
Biological Process peptidyl-serine phosphorylation Source:GO_Central1 Publication
Biological Process positive regulation of smoothened signaling pathway Source:UniProtKB2 Publications
Biological Process protein autophosphorylation Source:UniProtKB2 Publications
Biological Process smoothened signaling pathway Source:FlyBase1 Publication

Cellular Location

Cytoplasm
Localizes to pre-autophagosomal structure during cellular senescence.

PTM

Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ULK3 Recombinant Antibody (CBT3307)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ULK3 Recombinant Antibody (1404CT148-89-25) (CAT#: CBMAB-C4627-CN)

Rabbit Anti-ULK3 Recombinant Antibody (CBCNC-780) (CAT#: CBMAB-C4628-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry