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Mouse Anti-VIM.L Recombinant Antibody (14h7) (CBMAB-0032-CN)

This product is a mouse antibody that recognizes VIM.L of Xenopus laevis. The antibody 14h7 can be used for immunoassay techniques such as: BL, IF, IHC, WB.
See all VIM.L antibodies

Summary

Host Animal
Mouse
Specificity
Xenopus laevis, Xenopus
Clone
14h7
Antibody Isotype
IgG1
Application
Blocking, IF, IHC, WB

Basic Information

Immunogen
Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments).
Specificity
Xenopus laevis, Xenopus
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Vimentin L homeolog
Introduction
VIM1 is an unconventional methylcytosine-binding protein that interacts in vitro with 5mCpG- and 5mCpHpG-modified DNA (via its SRA domain), as well as recombinant histones (H2B, H3, H4, and HTR12) in plant extracts. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Entrez Gene ID
UniProt ID
Alternative Names
vim; vim1; vim2; vim-a; vim-b
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Biological Process
Astrocyte developmentIEA:Ensembl
Bergmann glial cell differentiationIEA:Ensembl
Cellular response to interferon-gammaIEA:Ensembl
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to muramyl dipeptideManual Assertion Based On ExperimentIMP:UniProtKB
Intermediate filament organizationISS:UniProtKB
Lens fiber cell developmentIEA:Ensembl
Negative regulation of neuron projection developmentIEA:Ensembl
Positive regulation of collagen biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mRNA stabilityManual Assertion Based On ExperimentIMP:UniProtKB
SMAD protein signal transductionIEA:Ensembl
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane
Involvement in disease
Cataract 30, multiple types (CTRCT30):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.
PTM
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
Phosphorylated by STK33 (PubMed:18811945).
Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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