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Mouse Anti-WASL Recombinant Antibody (5F4) (CBMAB-A9906-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
5F4
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
WASL (NP_003932, 97 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Introduction
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]
Entrez Gene ID
8976
UniProt ID
O00401
Alternative Names
DKFZp779G0847; MGC48327; N-WASP; NWASP
Function
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:9422512, PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828).
Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:9422512, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828).
Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512).
In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080).
Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity).
Plays a role in dendrite spine morphogenesis (By similarity).
Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear (PubMed:19767742).
Biological Process
Biological Process actin filament polymerization Source:UniProtKB1 Publication
Biological Process actin filament-based movement Source:GO_Central1 Publication
Biological Process actin polymerization or depolymerization Source:ProtInc1 Publication
Biological Process cell division Source:UniProtKB-KW
Biological Process dendritic spine morphogenesis Source:UniProtKB
Biological Process negative regulation of lymphocyte migration Source:CACAO1 Publication
Biological Process negative regulation of membrane tubulation Source:UniProtKB1 Publication
Biological Process positive regulation of Arp2/3 complex-mediated actin nucleation Source:InterPro
Biological Process positive regulation of clathrin-dependent endocytosis Source:BHF-UCL
Biological Process positive regulation of filopodium assembly Source:BHF-UCL
Biological Process positive regulation of transcription by RNA polymerase II Source:UniProtKB1 Publication
Biological Process protein-containing complex assembly Source:ProtInc1 Publication
Biological Process protein-containing complex localization Source:Ensembl
Biological Process regulation of postsynapse organization Source:Ensembl
Biological Process regulation of protein localization Source:Ensembl
Biological Process response to bacterium Source:Ensembl
Biological Process spindle localization Source:Ensembl
Biological Process vesicle budding from membrane Source:BHF-UCL
Biological Process vesicle organization Source:BHF-UCL
Biological Process vesicle transport along actin filament Source:BHF-UCL
Cellular Location
Cytoplasm, cytoskeleton
Nucleus
Cytoplasm
Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity).
Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm (By similarity).
PTM
Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.
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For research use only. Not intended for any clinical use.

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