Mouse Anti-ZP2 Recombinant Antibody (CBT4641) (V2LY-0625-LY1985)

Name: Mouse Anti-ZP2 Recombinant Antibody (CBT4641)
SKU: V2LY-0625-LY1985
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ZP2 Recombinant Antibody (CBT4641) (V2LY-0625-LY1985) in ELISA

Mouse Anti-ZP2 Recombinant Antibody (CBT4641) (V2LY-0625-LY1985) in WB

Mouse Anti-ZP2 Recombinant Antibody (CBT4641) (V2LY-0625-LY1985) in FC

Mouse Anti-ZP2 Recombinant Antibody (CBT4641) (V2LY-0625-LY1985) in IHC

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBT4641

Antibody Isotype

IgG1

Application

IHC, FC

Basic Information

Immunogen

Purified recombinant fragment of human ZP2 (AA: 624-745) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

7783

Function

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy (PubMed:29895852).
The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor (PubMed:29895852).

Biological Process

Biological Process binding of sperm to zona pellucida Source:UniProtKBBy Similarity
Biological Process prevention of polyspermy Source:UniProtKBBy Similarity

Cellular Location

Processed zona pellucida sperm-binding protein 2
Zona pellucida
Cell membrane

Involvement in disease

Oocyte maturation defect 6 (OOMD6):
An autosomal recessive infertility disorder characterized by oocyte fertilization failure, due to defective sperm-binding to an abnormally thin zona pellucida in patient oocytes.

Topology

Extracellular: 39-716
Helical: 717-736
Cytoplasmic: 737-745

PTM

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.
N-glycosylated.
O-glycosylated; contains sulfate-substituted glycans.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ZP2 Recombinant Antibody (2B9) (CAT#: CBMAB-Z0715-WJ)

Rat Anti-ZP2 Recombinant Antibody (CBWJZ-279) (CAT#: CBMAB-Z0717-WJ)

Mouse Anti-ZP2 Recombinant Antibody (CBWJZ-282) (CAT#: CBMAB-Z0720-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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