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Mouse Anti-EIF4A1 Recombinant Antibody (7H7) (CBMAB-Z0394-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
7H7
Antibody Isotype
IgG
Application
WB, IHC-P, IF

Basic Information

Immunogen
Synthetic Peptide of eIF4A1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:5,000
IF(ICC)1:50-1:200
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & PBS
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 4A1
Entrez Gene ID
Human1973
Mouse13681
Rat287436
UniProt ID
HumanP60842
MouseP60843
RatQ812C4
Alternative Names
Eukaryotic Translation Initiation Factor 4A1; ATP-Dependent RNA Helicase EIF4A-1; EIF-4A-I; EIF4A-I; EIF4A; DDX2A; Eukaryotic Translation Initiation Factor 4A, Isoform 1;
Research Area
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Biological Process
Cytoplasmic translational initiation Source: GO_Central
Cellular Location
Cytosol; Extracellular exosome; Cytoplasm; Eukaryotic translation initiation factor 4F complex; Membrane
More Infomation

Schmidt, T., Dabrowska, A., Waldron, J. A., Hodge, K., Koulouras, G., Gabrielsen, M., ... & Bushell, M. (2022). Purine-rich RNA sequences in the 5′ UTR site-specifically regulate eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. bioRxiv.

Li, F., Cao, K., Wang, M., Liu, Y., & Zhang, Y. (2022). Astragaloside IV exhibits anti-tumor function in gastric cancer via targeting circRNA dihydrolipoamide S-succinyltransferase (circDLST)/miR-489-3p/eukaryotic translation initiation factor 4A1 (EIF4A1) pathway. Bioengineered, 13(4), 10112-10123.

Wang, C., Leavenworth, J., Zhang, C., Liu, Z., Yuan, K. Y., Wang, Y., ... & Liu, R. (2022). Epigenetic regulation of EIF4A1 through DNA methylation and an oncogenic role of eIF4A1 through BRD2 signaling in prostate cancer. Oncogene, 41(19), 2778-2785.

Ercan, H., Schrottmaier, W. C., Pirabe, A., Schmuckenschlager, A., Pereyra, D., Santol, J., ... & Zellner, M. (2021). Platelet phenotype analysis of COVID-19 patients reveals progressive changes in the activation of integrin αIIbβ3, F13A1, the SARS-CoV-2 Target EIF4A1 and Annexin A5. Frontiers in cardiovascular medicine, 8.

Zhao, Y., Wang, Y., Chen, W., Bai, S., Peng, W., Zheng, M., ... & Luan, Z. (2021). Targeted intervention of eIF4A1 inhibits EMT and metastasis of pancreatic cancer cells via c-MYC/miR-9 signaling. Cancer Cell International, 21(1), 1-13.

Wang, J., Pu, J., Zhang, Y., Yao, T., Luo, Z., Li, W., ... & Deng, Y. (2020). Exosome-transmitted long non-coding RNA SENP3-EIF4A1 suppresses the progression of hepatocellular carcinoma. Aging (Albany NY), 12(12), 11550.

Howard, C. M., Estrada, M., Terrero, D., Tiwari, A. K., & Raman, D. (2020). Identification of cardiac glycosides as novel inhibitors of eIF4A1-mediated translation in triple-negative breast cancer cells. Cancers, 12(8), 2169.

Ma, X., Li, B., Liu, J., Fu, Y., & Luo, Y. (2019). Phosphoglycerate dehydrogenase promotes pancreatic cancer development by interacting with eIF4A1 and eIF4E. Journal of Experimental & Clinical Cancer Research, 38(1), 1-15.

Peters, T. L., Tillotson, J., Yeomans, A. M., Wilmore, S., Lemm, E., Jiménez-Romero, C., ... & Schatz, J. H. (2018). Target-Based Screening against eIF4A1 Reveals the Marine Natural Product Elatol as a Novel Inhibitor of Translation Initiation with In Vivo Antitumor ActivityNovel Screen for Translation Inhibitors Targeting eIF4A1. Clinical Cancer Research, 24(17), 4256-4270.

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For research use only. Not intended for any clinical use.

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