Human Recombinant B3GALTL protein, His Tag (V2LY-0526-LY2231)

Name: Human Recombinant B3GALTL protein, His Tag
SKU: V2LY-0526-LY2231
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant B3GALTL protein, His Tag consist of Amino Acid: 29-494 and predicts a molecular mass of 55.4 kDa.

Molecule Mass

55.4 kDa

Sequence

Amino Acid: 29-494

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Beta 3-Glucosyltransferase

Function

O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.

Biological Process

Fucose metabolic process Source: UniProtKB-KW
Protein O-linked fucosylation Source: Reactome

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Peters-plus syndrome (PTRPLS): An autosomal recessive disorder characterized by anterior eye-chamber abnormalities, disproportionate short stature, developmental delay, characteristic craniofacial features, cleft lip and/or palate.

Topology

Cytoplasmic: 1-6 aa
Helical: 7-27 aa
Lumenal: 28-498 aa

Zhang, A., Venkat, A., Taujale, R., Mull, J. L., Ito, A., Kannan, N., & Haltiwanger, R. S. (2021). Peters plus syndrome mutations affect the function and stability of human β1, 3-glucosyltransferase. Journal of Biological Chemistry, 100843.

Thu, C. T., Chung, J. Y., Dhawan, D., Vaiana, C. A., & Mahal, L. K. (2021). High-Throughput miRFluR Platform Identifies miRNA Regulating B3GLCT That Predict Peters’ Plus Syndrome Phenotype, Supporting the miRNA Proxy Hypothesis. ACS Chemical Biology.

Thu, C. T., Chung, J. Y., Dhawan, D., Vaiana, C. A., & Mahal, L. K. (2021). High-throughput analysis of B3GLCT regulation predicts phenotype of Peters' Plus Syndrome in line with the miRNA Proxy Hypothesis. bioRxiv.

Neupane, S., Goto, J., Berardinelli, S. J., Ito, A., Haltiwanger, R. S., & Holdener, B. C. (2021). Hydrocephalus in mouse B3glct mutants is likely caused by defects in multiple B3GLCT substrates in ependymal cells and subcommissural organ. Glycobiology.

Totoń-Żurańska, J., Kapusta, P., Rybak-Krzyszkowska, M., Lorenc, K., Machlowska, J., Skalniak, A., ... & Wołkow, P. P. (2019). Contribution of a Novel B3GLCT Variant to Peters Plus Syndrome Discovered by a Combination of Next-Generation Sequencing and Automated Text Mining. International journal of molecular sciences, 20(23), 6006.

Holdener, B. C., Percival, C. J., Grady, R. C., Cameron, D. C., Berardinelli, S. J., Zhang, A., ... & Haltiwanger, R. S. (2019). ADAMTS9 and ADAMTS20 are differentially affected by loss of B3GLCT in mouse model of Peters plus syndrome. Human molecular genetics, 28(24), 4053-4066.

Weh, E., Takeuchi, H., Muheisen, S., Haltiwanger, R. S., & Semina, E. V. (2017). Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome. PloS one, 12(9), e0184903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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