Human Recombinant CTF1 protein, hFc Tag (V2LY-0526-LY2581)

Name: Human Recombinant CTF1 protein, hFc Tag
SKU: V2LY-0526-LY2581
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CTF1 protein, hFc Tag consist of Amino Acid: 2-201 and predicts a molecular mass of 49.5 kDa.

Molecule Mass

49.5 kDa

Sequence

Amino Acid: 2-201

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cardiotrophin 1

Function

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Biological Process

Cell-cell signaling Source: ProtInc
Cell surface receptor signaling pathway Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Leukemia inhibitory factor signaling pathway Source: Ensembl
Muscle organ development Source: ProtInc
Neuron development Source: Ensembl
Neuron differentiation Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of tyrosine phosphorylation of STAT protein Source: CACAO

Cellular Location

Secreted

Matokhniuk, M. O., Limanskiy, О. V., Zhebel, V. M., & Starzhynska, O. L. (2019). Cardiotrophin-1 as a marker of function and myocardial condition in essential hypertension and chronic heart failure. Reports of Vinnytsia National Medical University, 23(1), 172-177.

Lang, C., Shu, X., Peng, L., & Yu, X. (2019). The ERK signaling pathway is involved in cardiotrophin-1-induced neural differentiation of human umbilical cord blood mesenchymal stem cells in vitro. Cytotechnology, 71(5), 977-988.

Watanabe, T., Konii, H., & Sato, K. (2018). Emerging roles of cardiotrophin-1 in the pathogenesis and biomarker of atherosclerosis. J, 1(1), 94-105.

Peng, L., Shu, X., Lang, C., & Yu, X. (2017). Cardiotrophin-1 stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through PI3K/Akt-dependent signaling pathways. Cytotechnology, 69(6), 933-941.

Bortolotti, F., Ruozi, G., Falcione, A., Doimo, S., Dal Ferro, M., Lesizza, P., ... & Giacca, M. (2017). In vivo functional selection identifies cardiotrophin-1 as a cardiac engraftment factor for mesenchymal stromal cells. Circulation, 136(16), 1509-1524.

Wang, D., Liu, X., Liu, Y., Li, S., & Wang, C. (2017). The effects of cardiotrophin-1 on early synaptic mitochondrial dysfunction and synaptic pathology in APPswe/PS1dE9 mice. Journal of Alzheimer's Disease, 59(4), 1255-1267.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CTF1 protein (CAT#: V2LY-0526-LY2580)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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