Human Recombinant DNASE1 protein, His Tag (V2LY-0526-LY3609)

Name: Human Recombinant DNASE1 protein, His Tag
SKU: V2LY-0526-LY3609
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant DNASE1 protein, His Tag consist of Amino Acid: 1-282 and predicts a molecular mass of 30.7 kDa.

Molecule Mass

30.7 kDa

Sequence

Amino Acid: 1-282

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Deoxyribonuclease 1

Function

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:2251263, PubMed:11241278, PubMed:2277032).

Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity).

Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278).

Binds specifically to G-actin and blocks actin polymerization (By similarity).

Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity).

NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity).

Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

Biological Process

Apoptotic process Source: UniProtKB-KW
DNA catabolic process Source: GO_Central
DNA catabolic process, endonucleolytic Source: UniProtKB
Neutrophil activation involved in immune response Source: UniProtKB
Regulation of acute inflammatory response Source: UniProtKB
Regulation of neutrophil mediated cytotoxicity Source: UniProtKB

Cellular Location

Secreted; Nucleus envelope; Zymogen granule. Secretory protein, stored in zymogen granules and found in the nuclear envelope.

Involvement in disease

Systemic lupus erythematosus (SLE):
Disease susceptibility is associated with variants affecting the gene represented in this entry. Neutrophil extracellular traps (NETs) are impaired in patients suffering from SLE (PubMed:20439745). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:20439745). A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.

Egli-Spichtig, D., Zhang, M. Y., Li, A., Pastor Arroyo, E. M., Hernando, N., Wagner, C. A., ... & Perwad, F. (2021). Renal Dnase1 expression is regulated by FGF23 but loss of Dnase1 does not alter renal phosphate handling. Scientific reports, 11(1), 1-14.

Engavale, M., McCord, J., Mapp, B., Nzimulinda, N., Bengtson, E., Sutton, R. B., & Keyel, P. A. (2021). Dnase1 Family in Autoimmunity. Encyclopedia, 1(3), 527-541.

Verhülsdonk, L., Mannherz, H. G., & Napirei, M. (2021). Comparison of the secretory murine DNase1 family members expressed in Pichia pastoris. Plos one, 16(7), e0253476.

Han, D. S., Ni, M., Chan, R. W., Chan, V. W., Lui, K. O., Chiu, R. W., & Lo, Y. D. (2020). The biology of cell-free DNA fragmentation and the roles of DNASE1, DNASE1L3, and DFFB. The American Journal of Human Genetics, 106(2), 202-214.

Kenny, E. F., Raupach, B., Abu Abed, U., Brinkmann, V., & Zychlinsky, A. (2019). Dnase1‐deficient mice spontaneously develop a systemic lupus erythematosus‐like disease. European Journal of Immunology, 49(4), 590-599.

Cheng, T. H., Lui, K. O., Peng, X. L., Cheng, S. H., Jiang, P., Chan, K. A., ... & Lo, Y. D. (2018). DNase1 does not appear to play a major role in the fragmentation of plasma DNA in a knockout mouse model. Clinical chemistry, 64(2), 406-408.

Pranzatelli, T. J., Michael, D. G., & Chiorini, J. A. (2018). ATAC2GRN: optimized ATAC-seq and DNase1-seq pipelines for rapid and accurate genome regulatory network inference. BMC genomics, 19(1), 1-13.

Boettcher, M., Meier, D., Jiménez-Alcázar, M., Eschenburg, G., Mietzsch, S., Vincent, D., ... & Fuchs, T. A. (2017). Degradation of extracellular DNA by DNase1 significantly reduces testicular damage after testicular torsion in rats. Urology, 109, 223-e1.

Gatselis, N. K., Vakrakou, A. G., Zachou, K., Androutsakos, T., Azariadis, K., Hatzis, G., ... & Dalekos, G. N. (2017). Decreased serum DNase1-activity in patients with autoimmune liver diseases. Autoimmunity, 50(2), 125-132.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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