Mouse Anti-4-HHE Recombinant Antibody (V2-502279) (CBMAB-C1054-CN)

Name: Mouse Anti-4-HHE Recombinant Antibody (V2-502279)
SKU: CBMAB-C1054-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-4-HHE Recombinant Antibody (HNEJ-2) in ELISA

ELISA study for the specificity of mAb HNEJ-2. Aldehyde- modified proteins were prepared by reacting protein (GAPDH) with 1 mM aldehyde (1 M for glutaraldehyde and formaldehyde) in 50 mM sodium phosphate buffer (pH 7.2) for 2 h at 37°C. Refer to section 2 for details. ...View More

Toyokuni, S., Miyake, N., Hiai, H., Hagiwara, M., Kawakishi, S., Osawa, T., & Uchida, K. (1995). The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct. FEBS letters, 359(2-3), 189-191.

Mouse Anti-4-HHE Recombinant Antibody (HNEJ-2) in WB

Western blot analysis of mAbs HNEJ-1 5 (200/tg/m]) using BSA and HNE-BSA (25 pg each/lane). (A) BSA; (B) HNE BSA; (P) polyclonal antibody published in [5] (2 pg/ml) as a control. 1 5 corre- spond to mAbs HNEJ-1 5, respectively. ...View More

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-502279

Application

IHC, WB

Immunogen

4-Hydroxy-2-nonenal (4-HNE) modified keyhole limpet hemocyanin.

Host Species

Mouse

Specificity

Chemical

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	15 µg/ml
IHC	25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

TBS

Preservative

None

Concentration

Batch dependent

More Infomation

Target

Full Name

4-HHE

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-4-HHE Recombinant Antibody (V2-502276) (CAT#: CBMAB-C1051-CN)

Mouse Anti-4-HHE Recombinant Antibody (V2-502277) (CAT#: CBMAB-C1052-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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