Mouse Anti-ADCYAP1 Recombinant Antibody (V2-356443) (CBMAB-P0026-YC)

Name: Mouse Anti-ADCYAP1 Recombinant Antibody (V2-356443)
SKU: CBMAB-P0026-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADCYAP1 Recombinant Antibody (CBYC-P006) in WB

Western blotting analysis of the expression of PACAP. Representative diagram for the sites of the prefrontal cortex transfection of PACAP RNAi or scramble control sequence (SCR). T-test, ∗P < 0.05 versus SCR group, n = 3. ...View More

Lu, L., Wu, D., Wang, K., Tang, J., & Chen, G. (2020). Beneficial effects of crocin against depression via pituitary adenylate cyclase-activating polypeptide. BioMed research international, 2020.

Mouse Anti-ADCYAP1 Recombinant Antibody (CBYC-P006) in IF

The PGD2 receptor DP1 (magenta) is expressed in vMPO neurons, co-localized with neural peptides BDNF (yellow) in putative soma and PACAP (cyan) in putative processes along the 3rd ventricle (3 replicates); scale bar, 50 µm. ...View More

Wang, T. A., Teo, C. F., Åkerblom, M., Chen, C., Tynan-La Fontaine, M., Greiner, V. J., ... & Jan, L. Y. (2019). Thermoregulation via temperature-dependent PGD2 production in mouse preoptic area. Neuron, 103(2), 309-322.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-356443

Application

WB, ICC, IP, ELISA

Immunogen

Amino acids 101-176 of PACAP of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenylate Cyclase Activating Polypeptide 1

Introduction

ADCYAP1 is a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses.

Entrez Gene ID

116

UniProt ID

P18509

Alternative Names

PACAP

Function

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).

Biological Process

Activation of adenylate cyclase activity
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
ATP metabolic process
Behavioral fear response
cAMP-mediated signaling
Cell-cell signaling
Cellular response to glucocorticoid stimulus
Female pregnancy
G protein-coupled receptor signaling pathway
Histamine secretion
Insulin secretion
Negative regulation of acute inflammatory response to antigenic stimulus
Negative regulation of acute inflammatory response to non-antigenic stimulus
Negative regulation of glial cell proliferation
Negative regulation of GTPase activity
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of muscle cell apoptotic process
Negative regulation of potassium ion transport
Neuron projection development
Neuropeptide signaling pathway
Ovarian follicle development
Pituitary gland development
Positive regulation of cAMP-mediated signaling
Positive regulation of cell population proliferation
Positive regulation of chemokine (C-C motif) ligand 5 production
Positive regulation of cold-induced thermogenesis
Positive regulation of cytosolic calcium ion concentration
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of growth hormone secretion
Positive regulation of GTPase activity
Positive regulation of interleukin-6 production
Positive regulation of neuron projection development
Positive regulation of protein kinase activity
Positive regulation of somatostatin secretion
Positive regulation of synaptic transmission, glutamatergic
Positive regulation of transcription by RNA polymerase II
Regulation of G protein-coupled receptor signaling pathway
Regulation of oligodendrocyte progenitor proliferation
Regulation of postsynaptic membrane potential
Regulation of protein localization
Response to ethanol
Response to starvation
Sensory perception of pain

Cellular Location

Secreted

Somoskői, B., Török, D., Reglődi, D., Tamás, A., Fülöp, B. D., & Cseh, S. (2020). Possible effects of pituitary adenylate cyclase activating polypeptide (PACAP) on early embryo implantation marker HB-EGF in mouse. Reproductive biology, 20(1), 9-13.

Gargiulo, A. T., Curtis, G. R., & Barson, J. R. (2020). Pleiotropic pituitary adenylate cyclase-activating polypeptide (PACAP): Novel insights into the role of PACAP in eating and drug intake. Brain research, 1729, 146626.

Cardoso, J. C. D. R., G García, M., & Power, D. M. (2020). Corrigendum: Tracing the Origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP). Frontiers in Neuroscience, 14, 801.

Johnson, G. C., Parsons, R., May, V., & Hammack, S. E. (2020). The role of pituitary adenylate cyclase-activating polypeptide (PACAP) signaling in the hippocampal dentate gyrus. Frontiers in Cellular Neuroscience, 14, 111.

Johnson, G. C., Parsons, R. L., May, V., & Hammack, S. E. (2020). Pituitary adenylate cyclase-activating polypeptide-induced PAC1 receptor internalization and recruitment of MEK/ERK signaling enhance excitability of dentate gyrus granule cells. American Journal of Physiology-Cell Physiology, 318(5), C870-C878.

Denes, V., Geck, P., Mester, A., & Gabriel, R. (2019). Pituitary adenylate cyclase-activating polypeptide: 30 years in research spotlight and 600 million years in service. Journal of clinical medicine, 8(9), 1488.

Johnson, G. C., May, V., Parsons, R. L., & Hammack, S. E. (2019). Parallel signaling pathways of pituitary adenylate cyclase activating polypeptide (PACAP) regulate several intrinsic ion channels. Annals of the New York Academy of Sciences, 1455(1), 105.

Mijiddorj, T., Kanasaki, H., Oride, A., Hara, T., Sukhbaatar, U., Tumurbaatar, T., & Kyo, S. (2017). Interaction between kisspeptin and adenylate cyclase-activating polypeptide 1 on the expression of pituitary gonadotropin subunits: a study using mouse pituitary lbetaT2 cells. Biology of reproduction, 96(5), 1043-1051.

Illés, A., Opper, B., Reglodi, D., Kerényi, M., Czetany, P., Boronkai, A., ... & Horváth, G. (2017). Effects of pituitary adenylate cyclase activating polypeptide on small intestinal INT 407 cells. Neuropeptides, 65, 106-113.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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