Mouse Anti-GRB10 Recombinant Antibody (4E6) (V2LY-0226-LY1050)

Name: Mouse Anti-GRB10 Recombinant Antibody (4E6)
SKU: V2LY-0226-LY1050
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4E6

Application

Immunogen

E. coli-derived recombinant protein, aa 1-282 of human GRB10.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

growth factor receptor-bound protein 10

Entrez Gene ID

Human	2887
Mouse	14783
Rat	498416

UniProt ID

Human	Q13322
Mouse	Q60760
Rat	P0CE43

Function

Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.

Biological Process

ERK1 and ERK2 cascade Source: Ensembl
Gene expression Source: Ensembl
Insulin-like growth factor receptor signaling pathway Source: Ensembl
Insulin receptor signaling pathway Source: BHF-UCL
Negative regulation of glucose import Source: BHF-UCL
Negative regulation of glycogen biosynthetic process Source: BHF-UCL
Negative regulation of insulin receptor signaling pathway Source: BHF-UCL
Negative regulation of phosphorylation Source: Ensembl
Negative regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of phosphorylation Source: BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
Response to insulin Source: BHF-UCL
Vascular associated smooth muscle cell migration Source: Ensembl

Cellular Location

Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity).

PTM

Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation (By similarity). Phosphorylated at Tyr-67 by TEC.

Dahiya, N., Kaur, M., Sharma, A., Singh, V., & Gupta, R. (2023, January). Network and Node Analysis of Growth factor receptor-binding protein 10: Insulin Signaling Pathway Regulator. In 2023 Third International Conference on Advances in Electrical, Computing, Communication and Sustainable Technologies (ICAECT) (pp. 1-4). IEEE.

Liu, H., He, Y., Bai, J., Zhang, C., Zhang, F., Yang, Y., ... & Xu, Y. (2023). Hypothalamic Grb10 enhances leptin signalling and promotes weight loss. Nature Metabolism, 1-18.

Yang, Y., Yao, H. J., Lin, W. J., Huang, S. C., Li, X. D., & He, F. Z. (2022). Real role of growth factor receptor-binding protein 10: Linking lipid metabolism to diabetes cardiovascular complications. World Journal of Clinical Cases, 10(35), 12875.

Chen, Y., Tang, M., Xiong, J., Gao, Q., Cao, W., & Huang, J. (2022). GRB10 is a novel oncogene associated with cell proliferation and prognosis in glioma. Cancer Cell International, 22(1), 1-13.

Cai, Z., Liu, F., Yang, Y., Li, D., Hu, S., Song, L., ... & Zhang, J. (2022). GRB10 regulates β-cell mass by inhibiting β-cell proliferation and stimulating β-cell dedifferentiation. Journal of Genetics and Genomics, 49(3), 208-216.

Wang, D., Ming, X., Xu, J., & Xiao, Y. (2021). Circ_0009910 shuttled by exosomes regulates proliferation, cell cycle and apoptosis of acute myeloid leukemia cells by regulating miR‐5195‐3p/GRB10 axis. Hematological Oncology, 39(3), 390-400.

Edick, A. M., Auclair, O., & Burgos, S. A. (2020). Role of Grb10 in mTORC1-dependent regulation of insulin signaling and action in human skeletal muscle cells. American Journal of Physiology-Endocrinology and Metabolism, 318(2), E173-E183.

Khan, M. I., Al Johani, A., Hamid, A., Ateeq, B., Manzar, N., Adhami, V. M., ... & Mukhtar, H. (2019). Proproliferative function of adaptor protein GRB10 in prostate carcinoma. The FASEB Journal, 33(3), 3198.

He, Z. Y., Wang, H. Y., Zhou, X., Liang, X. Y., Yan, B., Wang, R., ... & Wang, Y. L. (2018). Evaluation of vitrification protocol of mouse ovarian tissue by effect of DNA methyltransferase-1 and paternal imprinted growth factor receptor-binding protein 10 on signaling pathways. Cryobiology, 80, 89-95.

Hao, J., Ci, X., Xue, H., Wu, R., Dong, X., Choi, S. Y. C., ... & Wang, Y. (2018). Patient-derived hormone-naive prostate cancer xenograft models reveal growth factor receptor bound protein 10 as an androgen receptor-repressed gene driving the development of castration-resistant prostate cancer. European urology, 73(6), 949-960.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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