Sign in or Register   Sign in or Register
  |  

CEBPA

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Full Name
CCAAT/Enhancer Binding Protein Alpha
Function
Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107).
During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity).
Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity).
To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596).
In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC1. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).
Isoform 3:
Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation (PubMed:14660596).
Isoform 4:
Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
Biological Process
Brown fat cell differentiation Source: Ensembl
Cell maturation Source: Ensembl
Cellular response to lithium ion Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
Cholesterol metabolic process Source: Ensembl
Cytokine-mediated signaling pathway Source: UniProtKB
Embryonic placenta development Source: Ensembl
Fat cell differentiation Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Glucose homeostasis Source: UniProtKB
Granulocyte differentiation Source: UniProtKB
Inner ear development Source: Ensembl
Interleukin-6-mediated signaling pathway Source: ARUK-UCL
Lipid homeostasis Source: UniProtKB
Liver development Source: UniProtKB
Lung development Source: UniProtKB
Macrophage differentiation Source: Ensembl
Mitochondrion organization Source: Ensembl
Myeloid cell differentiation Source: GO_Central
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: ParkinsonsUK-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Notch signaling pathway Source: Ensembl
Positive regulation of DNA-templated transcription, initiation Source: UniProtKB
Positive regulation of fat cell differentiation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of inflammatory response Source: ARUK-UCL
Positive regulation of macrophage activation Source: ARUK-UCL
Positive regulation of osteoblast differentiation Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription by RNA polymerase III Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription by RNA polymerase I Source: UniProtKB
Urea cycle Source: Ensembl
viral process Source: UniProtKB-KW
white fat cell differentiation Source: Ensembl
Cellular Location
Nucleus
Isoform 4: Nucleolus
Involvement in disease
Leukemia, acute myelogenous (AML): A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes.
PTM
Phosphorylation at Ser-190 is required for interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibition. Dephosphorylated at Ser-190 by protein phosphatase 2A (PP2A) through PI3K/AKT signaling pathway regulation (PubMed:15107404). Phosphorylation at Thr-226 and Thr-230 by GSK3 is constitutive in adipose tissue and lung. In liver, both Thr-226 and Thr-230 are phosphorylated only during feeding but not during fasting. Phosphorylation of the GSK3 consensus sites selectively decreases transactivation activity on IRE-controlled promoters.
Sumoylated, sumoylation blocks the inhibitory effect on cell proliferation by disrupting the interaction with SMARCA2.
Ubiquitinated by COP1 upon interaction with TRIB1.

Anti-CEBPA antibodies

Loading...
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBWJC-2459
Application*: WB, IP, IF, E
Target: CEBPA
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: EP709Y
Application*: F, IP, WB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CBXC-2433
Application*: WB, F, IF, IP
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-1695
Application*: E, F, WB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: CBFYC-1694
Application*: WB, IF
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CBFYC-1693
Application*: IF, IP, WB
Target: CEBPA
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CBLC146-LY
Application*: WB, IP, P, IF, F
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYY-C1860
Application*: E, WB, P, IF, F
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: 8C68
Application*: WB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 692716
Application*: WB, IC
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 5B7
Application*: E, F, IC/IF, IH, P, WB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 4E10
Application*: WB, E, F
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 52D6a
Application*: DB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13B167
Application*: E, F, C, WB
Target: CEBPA
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13B166
Application*: E, C, WB
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
Go to
Compare