LAMP2 Antibodies

Structure of LAMP2

The LAMP2 protein is a lysosomal membrane protein with a molecular weight of approximately 45 kDa. This value varies slightly among different subtypes. The protein is composed of approximately 410 amino acid residues and its structure includes a cytoplasmic N-terminal tail, a highly glycosylated transmembrane domain, and a C-terminal domain that extends into the lysosomal lumen. Its primary structure exhibits characteristic glycoprotein features, with the extracellular domain rich in glycosylation modifications, which are crucial for maintaining protein stability and the integrity of the lysosomal membrane. The tertiary structure enables it to act as a bridging protein, facilitating the membrane fusion process between autophagosomes and lysosomes.

Fig. 1 LAMP2 protein and domains.¹

Key structural properties of myoglobin:

• Highly glycosylated intracellular domain
• Single transmembrane helical anchoring to the lysosomal membrane
• Cytoplasmic short-tailed form contains interaction sites for autophagy-related proteins

Functions of LAMP2

The main function of the LAMP2 gene is to maintain the integrity of lysosomes and regulate the autophagy process. However, it is also involved in a variety of crucial cellular physiological activities, including the clearance of intracellular metabolic waste, antigen presentation, and the regulation of apoptosis.

Unlike single-functional oxygen-binding proteins, LAMP2 integrates multiple interactions through its multiple domains, demonstrating a core role as a "multifunctional hub" in maintaining cellular homeostasis. The precise regulation of its expression level and function is crucial for cells to adapt to nutritional changes, stress responses, and tissue development.

Applications of LAMP2 and LAMP2 Antibody in Literature

1. Shalata, Adel, et al. "Danon Disease: Entire LAMP2 Gene Deletion with Unusual Clinical Presentation—Case Report and Review of the Literature." Genes 14.8 (2023): 1539. https://doi.org/10.3390/genes14081539

This article reports a father-daughter pair with Danon disease, presenting with mild clinical manifestations. The father was diagnosed with pre-excitation syndrome at the age of 13 and developed visual impairment at 25; the daughter was 3 years old and only showed mild electrocardiogram abnormalities. Genetic testing revealed a microdeletion in the Xq24 region affecting the LAMP2 gene. This article discusses the diagnosis, treatment, and management of this disease in conjunction with relevant literature.

2. Rodrigues, Pedro M., et al. "LAMP2 regulates autophagy in the thymic epithelium and thymic stroma-dependent CD4 T cell development." Autophagy 19.2 (2023): 426-439. https://doi.org/10.1080/15548627.2022.2074105

The research reveals that in the epithelial cells of the thymic cortex, LAMP2 regulates the autophagy-lysosome pathway, influencing the processing of self-peptide-MHC II complexes, thereby playing a crucial role in positive selection of CD4 T cells and the diversity of TCR receptor libraries.

3. Liu, Shan-peng, et al. "LAMP2 as a biomarker related to prognosis and immune infiltration in esophageal cancer and other cancers: a comprehensive pan-cancer analysis." Frontiers in oncology 12 (2022): 884448. https://doi.org/10.3389/fonc.2022.884448

This study, through pan-cancer analysis, revealed that LAMP2 is significantly upregulated in malignant tumors such as esophageal cancer. Its expression level is correlated with patient prognosis, clinical pathological features, and immune cell infiltration, suggesting that it can serve as a potential therapeutic target.

4. Zheng, Hao, et al. "Lamp2 inhibits epithelial-mesenchymal transition by suppressing Snail expression in HCC." Oncotarget 9.54 (2018): 30240. https://doi.org/10.18632/oncotarget.25367

The study found that LAMP2 is expressed at a low level in hepatocellular carcinoma and is associated with poor prognosis and metastasis in patients. Mechanistically, LAMP2 reverses epithelial-mesenchymal transition by inhibiting Snail expression, thereby suppressing tumor metastasis.

5. Sun, Chuanyu, et al. "Ferroptosis‐Related Prognostic Gene LAMP2 Is a Potential Biomarker Differential Expressed in Castration Resistant Prostate Cancer." Disease Markers 2023.1 (2023): 8295113. https://doi.org/10.1155/2023/8295113

Studies have shown that the iron death-related gene LAMP2 is significantly expressed in prostate cancer. Its expression level affects immune infiltration and is related to the survival prognosis of patients, and it may serve as a potential biomarker.

Creative Biolabs: LAMP2 Antibodies for Research

Creative Biolabs specializes in the production of high-quality LAMP2 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom LAMP2 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our LAMP2 antibodies, custom preparations, or technical support, contact us at info@creative-biolabs.com.