MTOR Antibodies

Structure of MTOR

MTOR is a large serine/threonine kinase with a molecular weight of approximately 289 kDa. Its molecular weight is highly conserved among different species, but in the human body, there are minor differences due to different splicing variants.

This protein is composed of over 2,500 amino acid residues, and its primary structure folds into a complex multi-domain three-dimensional conformation. The core of the MTOR protein is the kinase domain at the C-terminal, and its activity is regulated by the sensitivity of the upstream FRB domain to rapamycin. Multiple HEAT repeat sequences form superhelical structures at the N-terminal, mediating protein-protein interactions and complex assembly. Its functional exertion depends on the formation of two different complexes, mTORC1/2, and the specific adaptor proteins of both (such as Raptor and Rictor) determine the substrate specificity and the differences in signaling pathways.

Fig. 1 Schematic diagram of mTORC1 and mTORC2 structures.¹

Key structural properties of MTOR:

• Made up of multiple linear array HEAT repeat sequence superhelix stents
• With a conservative FRB structure domain for rapamycin mediated allosteric inhibition
• The C-terminal contains a typical kinase domain to catalyze substrate phosphorylation
• mTORC1/mTORC2 complexes with different functions were dynamically assembled by Raptor/Rictor

Functions of MTOR

The core function of MTOR kinase is to integrate intracellular and extracellular signals to regulate cell growth. However, it is also widely involved in a variety of physiological and pathological processes, including metabolic reprogramming, autophagy regulation and immune response.

The function of MTOR is achieved by forming two different complexes (MTORC1/2). mTORC1 is sensitive to rapamycin and is mainly responsible for regulating anabolism and autophagy. However, mTORC2 is not sensitive to rapamycin and mainly regulates cytoskeletal recombination and cell survival. The synergistic effect of the two determines the ultimate fate of cells.

Applications of MTOR and MTOR Antibody in Literature

1. Gargalionis, Antonios N., et al. "mTOR signaling components in tumor mechanobiology." International journal of molecular sciences 23.3 (2022): 1825. https://doi.org/10.3390/ijms23031825

The article indicates that mTOR, as a core hub integrating nutritional and metabolic signals, is abnormally activated in tumors. Recent studies have found that the mTOR pathway is interrelated with the mechanical and biological mechanisms of tumors. It regulates tumor growth, invasion and drug efficacy by sensing mechanical signals, providing a new perspective for cancer treatment.

2. Marafie, Sulaiman K., Fahd Al-Mulla, and Jehad Abubaker. "mTOR: its critical role in metabolic diseases, cancer, and the aging process." International Journal of Molecular Sciences 25.11 (2024): 6141. https://doi.org/10.3390/ijms25116141

The article indicates that mTOR is the core hub for regulating cell growth and metabolism, and its abnormal signals are closely related to cancer, metabolic diseases and aging. This article reviews the role of the mTOR pathway in diseases, explores the efficacy of existing inhibitors, and looks forward to the research and development prospects of the next generation of bifunctional targeted drugs.

3. Gao, Yuan, and Tian Tian. "mTOR signaling pathway and gut microbiota in various disorders: mechanisms and potential drugs in pharmacotherapy." International journal of molecular sciences 24.14 (2023): 11811. https://doi.org/10.3390/ijms241411811

The article indicates that mTOR, as a core regulatory factor of cellular metabolism, its abnormal signal is associated with various diseases. This review explores the mechanism by which the gut microbiota and its metabolites affect the functions of multiple organs such as the gastrointestinal tract and liver through the mTOR pathway, and looks forward to new strategies for treating related diseases by regulating the microbiota-mTOR axis.

4. Catena, Valeria, and Maurizio Fanciulli. "Deptor: not only a mTOR inhibitor." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 12. https://doi.org/10.1186/s13046-016-0484-y

The article indicates that Deptor is a key inhibitory protein of the mTOR complex and affects tumor development by regulating processes such as cell growth, autophagy and transcription. It plays a dual role in causing and inhibiting cancer. This review explores potential new therapeutic strategies targeting the Deptor-mTOR interaction.

5. Zeng, Chudai, et al. "The coordination of mTOR signaling and non-coding RNA in regulating epileptic neuroinflammation." Frontiers in Immunology 13 (2022): 924642. https://doi.org/10.3389/fimmu.2022.924642

The article indicates that the mTOR signaling pathway is a core regulatory factor of neuroinflammation in epilepsy. Non-coding Rnas participate in this process by interfering with the mTOR pathway, influencing neuroinflammatory cells and mediators, and thereby promoting the occurrence and development of epilepsy. This article reviews the interaction mechanism between the two in epilepsy and their potential therapeutic value.

Creative Biolabs: MTOR Antibodies for Research

Creative Biolabs specializes in the production of high-quality MTOR antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom MTOR Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MTOR antibodies, custom preparations, or technical support, contact us at email.