TBK1 Antibodies

Structure of TBK1

TBK1 is a serine/threonine kinase with a molecular weight of approximately 84 kDa. Its precise molecular weight varies slightly among different species due to differences in amino acid sequences.

This protein is composed of 729 amino acids and forms a typical kinase folding structure. Its N-terminal is the kinase catalytic domain, which forms the active center through an α -helix and β -folding. The C-terminal contains a coiled helical region that mediates protein interactions. The phosphorylation of the crucial serine residue at position 172 plays a core regulatory role in kinase activity, while the dimerization interface maintains its functional conformation, jointly fulfilling the signal transduction function.

Fig. 1 TBK1 protein structure and interaction partners.¹

Key structural properties of TBK1:

• The functional dimer is composed of the kinase domain and the C-terminal coil region
• Kinase domain structure forming inside the conservative ATP combined with pockets and substrate interface
• Phosphorylation site Ser172 is located in the activation link
• Two polymerization interface by hydrophobic interaction between spiral maintain signal complex assembly

Functions of TBK1

The core function of the TBK1 gene is to regulate the innate immune response and autophagy of cells. Meanwhile, it is also widely involved in the signal transduction of various pathophysiological processes.

The signal activation of TBK1 exhibits rapid transient characteristics, and its activity is strictly regulated by multiple phosphorylation modifications. This dynamic equilibrium feature makes it a core molecular switch of innate immunity.

Applications of TBK1 and TBK1 Antibody in Literature

1. Zhang, Min, et al. "Inhibitory targeting cGAS-STING-TBK1 axis: Emerging strategies for autoimmune diseases therapy." Frontiers in Immunology 13 (2022): 954129. https://doi.org/10.3389/fimmu.2022.954129

Research has found that abnormal activation of the cGAS-STING-TBK1 signal drives autoimmune diseases. A review of its key pathways, related diseases and the research and development progress of targeted inhibitors indicates that inhibiting this axis is a feasible therapeutic strategy.

2. Endo, Ryu, et al. "TBK1 adaptor AZI2/NAP1 regulates NDP52-driven mitochondrial autophagy." Journal of Biological Chemistry 300.10 (2024). https://doi.org/10.1016/j.jbc.2024.107775

Research reveals that in NDP52-driven mitochondrial autophagy, the adaptor protein AZI2 (rather than TBKBP1) and TBK1 work together to play a key role. AZI2 is recruited into damaged mitochondria and phosphorylated, a process that is crucial for mitochondrial degradation.

3. Vargas, Jose Norberto S., et al. "Spatiotemporal control of ULK1 activation by NDP52 and TBK1 during selective autophagy." Molecular cell 74.2 (2019): 347-362. https://doi.org/10.1016/j.molcel.2019.02.010

Research has revealed that the autophagy receptor NDP52, with the assistance of TBK1 kinase, can precisely recruit the ULK1 complex to damaged organelles, thereby initiating selective autophagy. This provides a key model for the core mechanism.

4. Wang, Banglu, et al. "TBK1 is paradoxical in tumor development: a focus on the pathway mediating IFN-I expression." Frontiers in Immunology 15 (2024): 1433321. https://doi.org/10.3389/fimmu.2024.1433321

Research reveals that TBK1 plays a "double-edged sword" role in tumor development. It can not only promote tumor survival but also mediate anti-tumor immunity by activating type I interferon (IFN). The bidirectness of the downstream IFN signal determines the complex function of TBK1.

5. Runde, Austin P., et al. "The role of TBK1 in cancer pathogenesis and anticancer immunity." Journal of Experimental & Clinical Cancer Research 41.1 (2022): 135. https://doi.org/10.1186/s13046-022-02352-y

The article indicates that TBK1 is a key kinase regulating innate immunity and cellular activities, and its abnormal activation can drive the occurrence of cancer. Studies have shown that inhibiting TBK1 can directly suppress cancer cells and activate T-cell immunity, making it a highly potential anti-cancer target.

Creative Biolabs: TBK1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality TBK1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom TBK1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our TBK1 antibodies, custom preparations, or technical support, contact us at email.