Mouse Anti-ACD Recombinant Antibody (V2-179248) (CBMAB-A0520-YC)

Name: Mouse Anti-ACD Recombinant Antibody (V2-179248)
SKU: CBMAB-A0520-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ACD Recombinant Antibody (1D8-1B6) (CBMAB-A0520-YC) in IP

Immunoprecipitation was carried out with an anti-TPP1 antibody. ...View More

Sandhu, R., Wei, D., Sharma, M., & Xu, L. (2019). An N-terminal Flag-tag impairs TPP1 regulation of telomerase function. Biochemical and biophysical research communications, 512(2), 230-235.

Mouse Anti-ACD Recombinant Antibody (1D8-1B6) (CBMAB-A0520-YC) in IF

Telomeric localization of Flag-tagged TPP1 examined by immunofluorescence-FISH analysis. Immunostaining was performed in HeLa1.2.11 cells expressing Flag-tagged TPP1 using an anti-Flag antibody. ...View More

Sandhu, R., Wei, D., Sharma, M., & Xu, L. (2019). An N-terminal Flag-tag impairs TPP1 regulation of telomerase function. Biochemical and biophysical research communications, 512(2), 230-235.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179248

Application

ELISA, IF, IHC-P, IP, WB

Immunogen

ACD (AAH16904, 1 a.a. ~ 544 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml
IHC	2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

adrenocortical dysplasia homolog (mouse)

Introduction

ACD is a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other comp

Entrez Gene ID

65057

UniProt ID

Q96AP0

Alternative Names

ACD, Shelterin Complex Subunit And Telomerase Recruitment Factor; POT1 And TIN2-Interacting Protein; TIN2 Interacting Protein 1; TINT1; PIP1; PTOP;

Function

Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity. May play a role in organogenesis.

Biological Process

Depurination
Establishment of protein localization to telomere
Intracellular protein transport
Negative regulation of DNA recombination at telomere
Negative regulation of telomere maintenance via telomerase
Positive regulation of histone exchange
Positive regulation of single-stranded telomeric DNA binding
Positive regulation of telomerase activity
Positive regulation of telomere maintenance via telomerase
Protection from non-homologous end joining at telomere
Protein localization to chromosome, telomeric region
Telomere assembly
Telomere capping
Telomere maintenance
Telomere maintenance via semi-conservative replication
Telomere maintenance via telomerase

Cellular Location

Nucleus; Telomere

Involvement in disease

A form of dyskeratosis congenita, a rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
A form of dyskeratosis congenita, a rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.

Henslee, G., Williams, C. L., Liu, P., & Bertuch, A. A. (2021). Identification and characterization of novel ACD variants: Modulation of TPP1 protein level offsets the impact of germline loss-of-function variants on telomere length. Molecular Case Studies, 7(1), a005454.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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