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Mouse Anti-ADA Recombinant Antibody (V2-630611) (CBMAB-BR010LY)

The product is antibody recognizes ADA. The antibody 6D4 immunoassay techniques such as: FC, IHC-P, WB.
See all ADA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-630611
Antibody Isotype
IgG2b
Application
FC, IHC-P, WB

Basic Information

Immunogen
E. coli-derived human ADA recombinant protein (Position: Q135-L363)
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.1-0.5 μg/ml
IHC-P0.5-1 μg/ml
FC1-3 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
0.05% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adenosine Deaminase
Introduction
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
Entrez Gene ID
100
UniProt ID
P00813
Alternative Names
Adenosine deaminase
Function
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development (By similarity).
Biological Process
Adenosine catabolic process
Aging
AMP catabolic process
Cell adhesion
dAMP catabolic process
dATP catabolic process
Deoxyadenosine catabolic process
Embryonic digestive tract development
Germinal center B cell differentiation
Histamine secretion
Hypoxanthine salvage
Inosine biosynthetic process
Liver development
Lung alveolus development
Negative regulation of adenosine receptor signaling pathway
Negative regulation of circadian sleep/wake cycle, non-REM sleep
Negative regulation of inflammatory response
Negative regulation of leukocyte migration
Negative regulation of mature B cell apoptotic process
Negative regulation of mucus secretion
Negative regulation of penile erection
Negative regulation of thymocyte apoptotic process
Peyer's patch development
Placenta development
Positive regulation of alpha-beta T cell differentiation
Positive regulation of B cell proliferation
Positive regulation of calcium-mediated signaling
Positive regulation of germinal center formation
Positive regulation of heart rate
Positive regulation of smooth muscle contraction
Positive regulation of T cell differentiation in thymus
Positive regulation of T cell receptor signaling pathway
Purine-containing compound salvage
Purine nucleotide salvage
Purine ribonucleoside monophosphate biosynthetic process
Regulation of cell-cell adhesion mediated by integrin
Response to drug
Response to hydrogen peroxide
Response to hypoxia
Response to morphine
Response to vitamin E
T cell activation
Trophectodermal cell differentiation
Xanthine biosynthetic process
Cellular Location
Cytoplasm; Cell membrane; Lysosome; Cell junction; Cytoplasmic vesicle lumen. Colocalized with DPP4 at the cell surface.
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID): An autosomal recessive disorder accounting for about 50% of non-X-linked SCIDs. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.

Odumade, O. A., Plotkin, A. L., Pak, J., Idoko, O. T., Pettengill, M. A., Kollmann, T. R., ... & Smolen, K. K. (2021). Plasma Adenosine Deaminase (ADA)-1 and-2 Demonstrate Robust Ontogeny Across the First Four Months of Human Life. Frontiers in immunology, 12, 2000.

Beukes, A., Shaw, J. A., Diacon, A. H., Irusen, E. M., & Koegelenberg, C. F. (2021). The utility of pleural fluid lactate dehydrogenase to adenosine deaminase ratio in pleural tuberculosis. Respiration, 100(1), 59-63.

Kuo, C. Y., Garabedian, E., Puck, J., Cowan, M. J., Sullivan, K. E., Buckley, R. H., ... & Kohn, D. B. (2020). Adenosine deaminase (ADA)–deficient severe combined immune deficiency (SCID) in the US Immunodeficiency Network (USIDNet) Registry. Journal of clinical immunology, 40(8), 1124-1131.

Fujiwara, S., Mimura, N., Yoshimura, H., Fujimoto, D., Ito, M., Mori, R., ... & Kohara, N. (2019). Elevated adenosine deaminase levels in the cerebrospinal fluid in immune checkpoint inhibitor-induced autoimmune encephalitis. Internal Medicine, 58(19), 2871-2874.

Kohn, D. B., Hershfield, M. S., Puck, J. M., Aiuti, A., Blincoe, A., Gaspar, H. B., ... & Grunebaum, E. (2019). Consensus approach for the management of severe combined immune deficiency caused by adenosine deaminase deficiency. Journal of Allergy and Clinical Immunology, 143(3), 852-863.

Flinn, A. M., & Gennery, A. R. (2018). Adenosine deaminase deficiency: a review. Orphanet journal of rare diseases, 13(1), 1-7.

Moreno, E., Canet, J., Gracia, E., Lluís, C., Mallol, J., Canela, E. I., ... & Casadó, V. (2018). Molecular evidence of adenosine deaminase linking adenosine A2A receptor and CD26 proteins. Frontiers in pharmacology, 9, 106.

Kohn, D. B., & Gaspar, H. B. (2017). How we manage adenosine deaminase-deficient severe combined immune deficiency (ADA SCID). Journal of clinical immunology, 37(4), 351-356.

Bradford, K. L., Moretti, F. A., Carbonaro-Sarracino, D. A., Gaspar, H. B., & Kohn, D. B. (2017). Adenosine deaminase (ADA)-deficient severe combined immune deficiency (SCID): molecular pathogenesis and clinical manifestations. Journal of clinical immunology, 37(7), 626-637.

Sousa, R., Serrano, P., Gomes Dias, J., Oliveira, J. C., & Oliveira, A. (2017). Improving the accuracy of synovial fluid analysis in the diagnosis of prosthetic joint infection with simple and inexpensive biomarkers: C-reactive protein and adenosine deaminase. The bone & joint journal, 99(3), 351-357.

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For research use only. Not intended for any clinical use.

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