What is the primary function and localization of the AGPAT2 protein?

AGPAT2 (1-acylglycerol-3-phosphate O-acyltransferase 2) is a critical enzyme in lipid metabolism. It catalyzes the second step in the de novo biosynthesis of triglycerides and glycerophospholipids by converting lysophosphatidic acid (LPA) into phosphatidic acid (PA). Due to its role in this pathway, it is an integral membrane protein localized primarily to the endoplasmic reticulum (ER). Mutations in AGPAT2 are the primary cause of type 1 congenital generalized lipodystrophy (CGL).

Is this antibody (CBMAB-A1603-YC) specific for AGPAT2? Will it cross-react with other AGPAT isoforms?

This antibody is a recombinant rabbit monoclonal, which generally provides high specificity. It was raised against human AGPAT2. While the AGPAT family has several members (e.g., AGPAT1, 3, 4, 5), this antibody is designed to be specific for AGPAT2. However, we always recommend running a parallel control, such as a lysate from a cell line with known high/low expression (e.g., human adipose tissue as a positive control) or a knockout/knockdown (KO/KD) lysate, to confirm specificity in your experimental model.

Rabbit Anti-AGPAT2 Recombinant Antibody (V2-180182) (CBMAB-A1603-YC)

See all AGPAT2 antibodies

Compare Online Inquiry

Published Data

Rabbit Anti-AGPAT2 Recombinant Antibody (CBYC-A417) (CBMAB-A1603-YC) in Immunoblot

Effect of targeting PA producing enzymes on intracellular level of PA in ovarian cancer cells. Major isoforms of DGK, LPAAT, and PLD were stably knocked down, cells were treated with sublethal doses of cisplatin, and PA levels quantified. (A-E) n=3 technical replicates. ...View More

Li, J., Pan, C., Boese, A. C., Kang, J., Umano, A. D., Magliocca, K. R., ... & Kang, S. (2020). DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 SignalingPhosphatidic Acid Promotes Chemotherapy Resistance. Clinical Cancer Research, 26(14), 3843-3855.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-180182

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues surrounding Gly235 of human AGPAT2 protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

1-Acylglycerol-3-Phosphate O-Acyltransferase 2

Introduction

AGPAT2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.

Entrez Gene ID

10555

UniProt ID

O15120

Alternative Names

1-Acylglycerol-3-Phosphate O-Acyltransferase 2; 1-Acylglycerol-3-Phosphate O-Acyltransferase 2 (Lysophosphatidic Acid Acyltransferase, Beta); 1-AGP Acyltransferase 2; LPAAT-Beta; 1-AGPAT 2; 1-Acyl-Sn-Glycerol-3-Phosphate Acyltransferase Beta; Lysophosphat

Function

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.

Biological Process

CDP-diacylglycerol biosynthetic process
Epidermis development
Neutrophil degranulation
Phosphatidic acid biosynthetic process
Phospholipid metabolic process
Positive regulation of cytokine-mediated signaling pathway
Positive regulation of cytokine production
Response to drug

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Congenital generalized lipodystrophy 1 (CGL1): An autosomal recessive disorder characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

Topology

Lumenal: 24-29 aa
Helical: 30-50 aa
Cytoplasmic: 51-121 aa
Helical: 122-142 aa
Lumenal: 143-278 aa

Junior, R. M. M., Lima, G. E. D. C. P., Fernandes, V. O., Montenegro, A. P. D. R., Ponte, C. M. M., Martins, L. V., ... & d’Alva, C. B. (2020). Leu124Serfs* 26, a novel AGPAT2 mutation in congenital generalized lipodystrophy with early cardiovascular complications. Diabetology & metabolic syndrome, 12(1), 1-9.

Tapia, P. J., Figueroa, A. M., Eisner, V., González-Hódar, L., Robledo, F., Agarwal, A. K., ... & Cortés, V. (2020). Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology. Metabolism, 111, 154341.

Ceccarini, G., Magno, S., Pelosini, C., Ferrari, F., Sessa, M. R., Scabia, G., ... & Santini, F. (2020). Congenital generalized lipoatrophy (Berardinelli-Seip syndrome) type 1: description of novel AGPAT2 homozygous variants showing the highly heterogeneous presentation of the disease. Frontiers in endocrinology, 11, 39.

Oswiecimska, J., Dawidziuk, M., Gambin, T., Ziora, K., Marek, M., Rzonca, S., ... & Gawlinski, P. (2019). A Patient with Berardinelli-Seip Syndrome, novel AGPAT2 splicesite mutation and concomitant development of non-diabetic polyneuropathy. Journal of clinical research in pediatric endocrinology, 11(3), 319.

Hasani-Ranjbar, S., Soltani, A., Hadavi, M., Ejtahed, H. S., Mohammad-Amoli, M., & Radmard, A. R. (2017). Congenital generalized lipodystrophy in a youth presented with sclerotic and lytic bone lesions; a family with AGPAT2 mutation. International Journal of Pediatrics, 5(2), 4275-4284.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-AGPAT2 Recombinant Antibody (V2-180182)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry