Sign in or Register   Sign in or Register
  |  

Rabbit Anti-AGPAT2 Recombinant Antibody (V2-180182) (CBMAB-A1603-YC)

Provided herein is a Rabbit monoclonal antibody against Human 1-Acylglycerol-3-Phosphate O-Acyltransferase 2. The antibody can be used for immunoassay techniques, such as WB, IP.
See all AGPAT2 antibodies
Published Data
Rabbit Anti-AGPAT2 Recombinant Antibody (CBYC-A417) (CBMAB-A1603-YC) in Immunoblot
Effect of targeting PA producing enzymes on intracellular level of PA in ovarian cancer cells. Major isoforms of DGK, LPAAT, and PLD were stably knocked down, cells were treated with sublethal doses of cisplatin, and PA levels quantified. (A-E) n=3 technical replicates. ...View More
Li, J., Pan, C., Boese, A. C., Kang, J., Umano, A. D., Magliocca, K. R., ... & Kang, S. (2020). DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 SignalingPhosphatidic Acid Promotes Chemotherapy Resistance. Clinical Cancer Research, 26(14), 3843-3855.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-180182
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly235 of human AGPAT2 protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
1-Acylglycerol-3-Phosphate O-Acyltransferase 2
Introduction
AGPAT2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.
Entrez Gene ID
10555
UniProt ID
O15120
Alternative Names
1-Acylglycerol-3-Phosphate O-Acyltransferase 2; 1-Acylglycerol-3-Phosphate O-Acyltransferase 2 (Lysophosphatidic Acid Acyltransferase, Beta); 1-AGP Acyltransferase 2; LPAAT-Beta; 1-AGPAT 2; 1-Acyl-Sn-Glycerol-3-Phosphate Acyltransferase Beta; Lysophosphat
Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Biological Process
CDP-diacylglycerol biosynthetic process
Epidermis development
Neutrophil degranulation
Phosphatidic acid biosynthetic process
Phospholipid metabolic process
Positive regulation of cytokine-mediated signaling pathway
Positive regulation of cytokine production
Response to drug
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Congenital generalized lipodystrophy 1 (CGL1): An autosomal recessive disorder characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Topology
Lumenal: 24-29 aa
Helical: 30-50 aa
Cytoplasmic: 51-121 aa
Helical: 122-142 aa
Lumenal: 143-278 aa

Junior, R. M. M., Lima, G. E. D. C. P., Fernandes, V. O., Montenegro, A. P. D. R., Ponte, C. M. M., Martins, L. V., ... & d’Alva, C. B. (2020). Leu124Serfs* 26, a novel AGPAT2 mutation in congenital generalized lipodystrophy with early cardiovascular complications. Diabetology & metabolic syndrome, 12(1), 1-9.

Tapia, P. J., Figueroa, A. M., Eisner, V., González-Hódar, L., Robledo, F., Agarwal, A. K., ... & Cortés, V. (2020). Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology. Metabolism, 111, 154341.

Ceccarini, G., Magno, S., Pelosini, C., Ferrari, F., Sessa, M. R., Scabia, G., ... & Santini, F. (2020). Congenital generalized lipoatrophy (Berardinelli-Seip syndrome) type 1: description of novel AGPAT2 homozygous variants showing the highly heterogeneous presentation of the disease. Frontiers in endocrinology, 11, 39.

Oswiecimska, J., Dawidziuk, M., Gambin, T., Ziora, K., Marek, M., Rzonca, S., ... & Gawlinski, P. (2019). A Patient with Berardinelli-Seip Syndrome, novel AGPAT2 splicesite mutation and concomitant development of non-diabetic polyneuropathy. Journal of clinical research in pediatric endocrinology, 11(3), 319.

Hasani-Ranjbar, S., Soltani, A., Hadavi, M., Ejtahed, H. S., Mohammad-Amoli, M., & Radmard, A. R. (2017). Congenital generalized lipodystrophy in a youth presented with sclerotic and lytic bone lesions; a family with AGPAT2 mutation. International Journal of Pediatrics, 5(2), 4275-4284.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-AGPAT2 Recombinant Antibody (V2-180182)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare