Rabbit Anti-ALCAM Recombinant Antibody (
21B9) (V2LY-0725-LY1208)

Name: Rabbit Anti-ALCAM Recombinant Antibody (21B9)
SKU: V2LY-0725-LY1208
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ALCAM Recombinant Antibody (21B9) (V2LY-0725-LY1208) in WB

Western Blot
Positive WB detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue
All lanes: ALCAM antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 64, 16, 34 kDa
Observed band size: 100 kDa

Rabbit Anti-ALCAM Recombinant Antibody (21B9) (V2LY-0725-LY1208) in IHC

IHC image of V2LY-0725-LY1208 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ALCAM Recombinant Antibody (21B9) (V2LY-0725-LY1208) in IHC

IHC image of V2LY-0725-LY1208 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

21B9

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human ALCAM.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Activated Leukocyte Cell Adhesion Molecule

Entrez Gene ID

214

UniProt ID

Q13740

Alternative Names

Activated leukocyte cell adhesion molecule, ALCAM, CD166.

Function

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Axon extension involved in axon guidance Source: UniProtKB
Cell adhesion Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Motor neuron axon guidance Source: Ensembl
Neuron projection extension Source: UniProtKB
Retinal ganglion cell axon guidance Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cell membrane; Axon; Dendrite. Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938).
Isoform 3: Secreted

Topology

Extracellular: 28-527 aa
Helical: 528-549 aa
Cytoplasmic: 550-583 aa

PTM

Glycosylated.

Zarghami, N., Soto, M. S., Perez-Balderas, F., Khrapitchev, A. A., Karali, C. S., Johanssen, V. A., ... & Sibson, N. R. (2021). A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models. Journal of Cerebral Blood Flow & Metabolism, 41(7), 1592-1607.

Darvishi, B., Boroumandieh, S., Majidzadeh-A, K., Salehi, M., Jafari, F., & Farahmand, L. (2020). The role of activated leukocyte cell adhesion molecule (ALCAM) in cancer progression, invasion, metastasis and recurrence: A novel cancer stem cell marker and tumor-specific prognostic marker. Experimental and molecular pathology, 115, 104443.

Xie, S., Zhang, H., Wang, F., Liu, Y., Gao, K., Zhang, J., ... & Jiang, W. (2020). Activated leukocyte cell adhesion molecule as a biomarker for disease severity and efficacy of sublingual immunotherapy in allergic rhinitis. International Immunopharmacology, 88, 106975.

Achiha, T., Kijima, N., Kodama, Y., Kagawa, N., Kinoshita, M., Fujimoto, Y., ... & Kishima, H. (2020). Activated leukocyte cell adhesion molecule expression correlates with the WNT subgroup in medulloblastoma and is involved in regulating tumor cell proliferation and invasion. PloS one, 15(12), e0243272.

Sanders, A. J., Owen, S., Morgan, L. D., Ruge, F., Collins, R. J., Ye, L., ... & Jiang, W. G. (2019). Importance of activated leukocyte cell adhesion molecule (ALCAM) in prostate cancer progression and metastatic dissemination. Oncotarget, 10(59), 6362.

Ferragut, F., Cagnoni, A. J., Colombo, L. L., Terrero, C. S., Wolfenstein-Todel, C., Troncoso, M. F., ... & Elola, M. T. (2019). Dual knockdown of Galectin-8 and its glycosylated ligand, the activated leukocyte cell adhesion molecule (ALCAM/CD166), synergistically delays in vivo breast cancer growth. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(8), 1338-1352.

Oh, M. S., Hong, J. Y., Kim, M. N., Kwak, E. J., Kim, S. Y., Kim, E. G., ... & Sohn, M. H. (2019). Activated leukocyte cell adhesion molecule modulates Th2 immune response in atopic dermatitis. Allergy, asthma & immunology research, 11(5), 677-690.

Jeong, Y. J., Oh, H. K., Park, S. H., & Bong, J. G. (2018). Prognostic significance of activated leukocyte cell adhesion molecule (ALCAM) in association with promoter methylation of the ALCAM gene in breast cancer. Molecules, 23(1), 131.

Kim, M. N., Hong, J. Y., Shim, D. H., Sol, I. S., Kim, Y. S., Lee, J. H., ... & Sohn, M. H. (2018). Activated leukocyte cell adhesion molecule stimulates the T-cell response in allergic asthma. American journal of respiratory and critical care medicine, 197(8), 994-1008.

Devis, L., Moiola, C. P., Masia, N., Martinez‐Garcia, E., Santacana, M., Stirbat, T. V., ... & Colas, E. (2017). Activated leukocyte cell adhesion molecule (ALCAM) is a marker of recurrence and promotes cell migration, invasion, and metastasis in early‐stage endometrioid endometrial cancer. The Journal of pathology, 241(4), 475-487.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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