Mouse Anti-ALDOC Recombinant Antibody (H11) (CBMAB-1063-CN)

Name: Mouse Anti-ALDOC Recombinant Antibody (H11)
SKU: CBMAB-1063-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALDOC Recombinant Antibody (H11) in IF

Structural loss of cerebellar PCs at the age of 5 months in APPswe/PSEN1dE9 mice. A. Representative fluorescent microscopic images (20X magnification, scale bar 100 μm) of cerebellar PCs stained with Aldolase C (green) and β3 Tubulin (red) in AD and wild type mice (WT). B. Quantitative analysis of PCs count, C. Perimeter (μM) of PCs. D. Primary dendritic branches length (μM) of cerebellar PCs in AD and WT mice. AD: n=5 mice; WT: n=4 mice; * p<0.05 compared to WT. ...View More

Chaudhari, K., Wang, L., Kruse, J., Winters, A., Sumien, N., Shetty, R., ... & Yang, S. H. (2021). Early loss of cerebellar Purkinje cells in human and a transgenic mouse model of Alzheimer's disease. Neurological research, 43(7), 570-581.

Mouse Anti-ALDOC Recombinant Antibody (H11) in WB

Western blot validation for the glycolytic proteins in P19 WT and EDITED cells. ...View More

Hu, X., Ono, M., Chimge, N. O., Chosa, K., Nguyen, C., Melendez, E., ... & Kahn, M. (2021). Differential Kat3 usage orchestrates the integration of cellular metabolism with differentiation. Cancers, 13(23), 5884.

Mouse Anti-ALDOC Recombinant Antibody (H11) in IHC

Dilution of parvalbumin antibody can affect intracellular staining appearance.
(A) Macroscopic view of the rostral cerebellum reveals even staining across lobules with fluorescent Nissl, displaying proper perfusion and all Purkinje cell bodies. Intracellular parvalbumin expression with fluorescence is difficult to appreciate from a macroscopic view, but shows that general labeling for interneurons and white matter tracts is even throughout the section. (B) Inset from A showing two rows of Purkinje cells in the vermis that display mostly parvalbumin-positive Purkinje cells (upper). (C) and mostly parvalbumin-negative/low Purkinje cells (lower) (D). Panel A and B are taken at 20× magnification and panels C and D are taken at 63× magnification on a Zeiss confocal spinning disc. In the green channel, the parvalbumin guinea pig was concentrated at 1:300, and although cells are often still empty at higher magnification, they appear to have more intracellular stain in some (arrows in D), suggesting expression levels may be lower and therefore antibody titrations may have significant consequences for counting cells as positive or negative/low. Parvalbumin mouse (Sigma) staining at 1:3000 replicates the pattern shown with the diluted parvalbumin guinea pig antibody. All Purkinje cells have equal Nissl staining. Together, this suggests that differential antibody labeling from different companies, angle of cut of the cell and perfusion artifacts are not likely explanations for differences in parvalbumin expression. Areas of the vermis are labeled as well as the paramedian lobule (Para). (E) Crus II staining of aldolase C in comparison to parvalbumin to show that both parvalbumin groups have aldolase C positive and negative cells represented. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) ...View More

Brandenburg, C., Smith, L. A., Kilander, M. B., Bridi, M. S., Lin, Y. C., Huang, S., & Blatt, G. J. (2021). Parvalbumin subtypes of cerebellar Purkinje cells contribute to differential intrinsic firing properties. Molecular and Cellular Neuroscience, 115, 103650.

Mouse Anti-ALDOC Recombinant Antibody (H11) in WB

Western blots of the astrocyte markers GFAP, S100B, V/vimentin, and Aldolase C ...View More

Bejoy, J., Bijonowski, B., Marzano, M., Jeske, R., Ma, T., & Li, Y. (2020). Wnt-Notch signaling interactions during neural and astroglial patterning of human stem cells. Tissue Engineering Part A, 26(7-8), 419-431.

Mouse Anti-ALDOC Recombinant Antibody (H11) in IF

Aldolase C was expressed mainly in neurons and astrocytes, but not in microglia. ...View More

Ruan, W., Hu, J., Zhou, H., Li, Y., Xu, C., Luo, Y., ... & Chen, G. (2020). Intranasal wnt-3a alleviates neuronal apoptosis in early brain injury post subarachnoid hemorrhage via the regulation of wnt target PPAN mediated by the moonlighting role of aldolase C. Neurochemistry international, 134, 104656.

Mouse Anti-ALDOC Recombinant Antibody (H11) in IHC

Characterization of ip-FN projections from the ip-PMv. Sagittal sections of the ip-FN of an intact (A, C, E; Monkey G) and lesioned monkey (B, D, F-H; Monkey F). Nissl-stained (A, B) and adjacent sections showing Aldoc (C, D), PLCB3 (E, F), and BDA labeling (G). Summary montage of these sagittal sections from the lesioned monkey illustrating the heterogeneity of the FN based on differential expression of Aldoc and PLCB3 (H). Areas surrounded by dotted and solid lines indicate the negative and positive zone of Aldoc (intact, black; lesioned, yellow) or PLCB3 (intact, black; lesioned, red), respectively. Scale bars: 300 μm. ...View More

Yamamoto, T., Hayashi, T., Murata, Y., Ose, T., & Higo, N. (2019). Premotor cortical-cerebellar reorganization in a macaque model of primary motor cortical lesion and recovery. Journal of Neuroscience, 39(43), 8484-8496.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

H11

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 77-112 within an internal region of Aldolase C of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldolase, Fructose-Bisphosphate C

Introduction

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Entrez Gene ID

230

UniProt ID

P09972

Alternative Names

ALDC

Biological Process

Canonical glycolysis Source: Reactome
Epithelial cell differentiation Source: UniProtKB
Fructose 1,6-bisphosphate metabolic process Source: UniProtKB
Fructose metabolic process Source: ProtInc
Gluconeogenesis Source: Reactome
Glycolytic process Source: GO_Central
Neutrophil degranulation Source: Reactome

Cellular Location

Cytoskeleton; Cytosol; Extracellular exosome; Extracellular region; Ficolin-1-rich granule lumen; Secretory granule lumen; Tertiary granule lumen

Izraely, S., Ben‐Menachem, S., Sagi‐Assif, O., Meshel, T., Malka, S., Telerman, A., ... & Witz, I. P. (2021). The melanoma brain metastatic microenvironment: aldolase C partakes in shaping the malignant phenotype of melanoma cells–a case of inter‐tumor heterogeneity. Molecular Oncology, 15(5), 1376-1390.

Yuan, J., Xing, H., Li, Y., Song, Y., Zhang, N., Xie, M., ... & Yang, M. (2021). EPB41 suppresses the Wnt/β-catenin signaling in non-small cell lung cancer by sponging ALDOC. Cancer Letters, 499, 255-264.

Chang, Y. C., Tsai, H. F., Huang, S. P., Chen, C. L., Hsiao, M., & Tsai, W. C. (2019). Enrichment of aldolase C correlates with low non-mutated IDH1 expression and predicts a favorable prognosis in glioblastomas. Cancers, 11(9), 1238.

Pamidimukkala, N. V., Leonard, M. K., Snyder, D., Mccorkle, J. R., & Kaetzel, D. M. (2018). Metastasis suppressor NME1 directly activates transcription of the ALDOC gene in melanoma cells. Anticancer research, 38(11), 6059-6068.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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