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Mouse Anti-ANLN Recombinant Antibody (CL0301) (CBMAB-A2814-YC)

Provided herein is a Mouse monoclonal antibody against Human Anillin Actin Binding Protein. The antibody can be used for immunoassay techniques, such as WB, IHC, IHC-P.
See all ANLN antibodies
Published Data
Mouse Anti-ANLN Recombinant Antibody (CL0301) in IHC
Representative image of ANLN immunohistochemical staining in ESCC and normal tissues (scale 200 and 50 μm). ...View More
Cao, Y. F., Xie, L., Tong, B. B., Chu, M. Y., Shi, W. Q., Li, X., ... & Li, E. M. (2023). Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. Cell Death & Differentiation, 30(2), 527-543.
Mouse Anti-ANLN Recombinant Antibody (CL0301) in IP
The indicated plasmids were transfected into HEK293T cells for 48 h, and then harvested with EBC buffer for co-immunoprecipitation. ...View More
Cao, Y. F., Xie, L., Tong, B. B., Chu, M. Y., Shi, W. Q., Li, X., ... & Li, E. M. (2023). Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. Cell Death & Differentiation, 30(2), 527-543.
Mouse Anti-ANLN Recombinant Antibody (CL0301) in IF
Anillin recruits CIN85 to the ICB membrane. (A) Dividing HeLa cells fixed and stained with antibodies recognizing anillin, CIN85, and tubulin. A yellow arrow points to ICB membrane localization, and a white arrow points to stem body localization.
(B) Stills from the video of a dividing GFP-CIN85-expressing cell. See also Video S1.
(C) Dividing HeLa cells fixed and stained with antibodies recognizing anillin, CD2AP, and α-tubulin. White arrows points to stem body localization.
(D) Stills from the video of a dividing CD2AP-GFP-expressing cell. See also Video S2.
(E) GFP-anillin- and GFP-anillin-R32A-expressing cells depleted of endogenous anillin using siRNA and then fixed and stained with the indicated antibodies.
(F) GFP-anillin- and GFP-anillin-R32A-expressing cells depleted of endogenous anillin using siRNA and then fixed and stained with the indicated antibodies.
Scale bars for whole-cell images measure 5 μm; 2 μm for magnifications. ...View More
Panagiotou, T. C., Chen, A., & Wilde, A. (2022). An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis. Cell Reports, 40(9).
Mouse Anti-ANLN Recombinant Antibody (CL0301) in Immunoblot
Immunoblotting of GFP, supervillin, and anillin in RNAi-treated HeLa cells expressing ezrin-EGFP. β-tubulin was detected as a loading control. ...View More
Yang, G., Hiruma, S., Kitamura, A., Kinjo, M., Mishra, M., & Uehara, R. (2021). Molecular basis of functional exchangeability between ezrin and other actin-membrane associated proteins during cytokinesis. Experimental Cell Research, 403(2), 112600.
Mouse Anti-ANLN Recombinant Antibody (CL0301) in IF
Septin marks the presence of an ICB. M-cells were isolated and either re-plated on fibronectin-coated dishes (adhesion) or non-adhesive dishes (suspension) for the indicated time points. Representative immunofluorescence images illustrating the localization of septin-7 (green) and anillin (red) in the adhesion and suspension conditions in a BJ cells, c BJ-LT cells, and e BJ-LT-Ras cells. Nuclei were stained with DAPI (blue). Note that for BJ and BJ-LT-Ras two examples are given at the 42- and 3-h time points, respectively, illustrating different septin-7 staining. Under adherent conditions, all three cell types had divided after 3 h (here shown only for BJ cells; see Additional file 5: Figure S3). b, d, f Mean% ± SD of the number of cells without septin-7 along ICB at the indicated time points for non-adherent BJ, BJ-LT, and BJ-LT-Ras cells. ...View More
Gupta, D. K., Kamranvar, S. A., Du, J., Liu, L., & Johansson, S. (2019). Septin and Ras regulate cytokinetic abscission in detached cells. Cell Division, 14, 1-15.
Mouse Anti-ANLN Recombinant Antibody (CL0301) in IHC
A Representative image of ANLN IHC staining (a&e, negative control b&f normal mucosa, c&g low expression of ANLN in CRC, d&h high expression of ANLN in CRC. B The immunoreactive score (IRS) of ANLN in colorectal cancer and normal colorectal mucosa. C Kaplan-Meier survival analysis displays the relationship of ANLN expression and overall-survival of CRC patients. ...View More
Wang, G., Shen, W., Cui, L., Chen, W., Hu, X., & Fu, J. (2016). Overexpression of Anillin (ANLN) is correlated with colorectal cancer progression and poor prognosis. Cancer Biomarkers, 16(3), 459-465.
Mouse Anti-ANLN Recombinant Antibody (CL0301) in Immunoblot
Parental MCF-7 cells were mixed with Snail6SA cells transfected with control (ctrl), Anilin, p190BGAP, RhoA or NMIIA specific siRNA, and immunoblotted for the corresponding siRNA targets. ...View More
Wee, K., Hediyeh-Zadeh, S., Duszyc, K., Verma, S., Nanavati, B. N., Khare, S., ... & Budnar, S. (2020). Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion. Journal of cell science, 133(13), jcs235622.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CL0301
Antibody Isotype
IgG2b, κ
Application
WB, IP, IHC-P, IF, ELISA

Basic Information

Immunogen
Amino acids 641-940 mapping near the C-terminus of Anillin of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anillin Actin Binding Protein
Introduction
ANLN is an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated w
Entrez Gene ID
54443
UniProt ID
Q9NQW6
Alternative Names
Anillin Actin Binding Protein; Anillin, Actin Binding Protein (Scraps Homolog, Drosophila); Anillin (Drosophila Scraps Homolog), Actin Binding Protein; Actin-Binding Protein Anillin; Anillin; Scraps; FSGS8; Scra;
Function
Required for cytokinesis (PubMed:16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed:23870127). May play a significant role in podocyte cell migration (PubMed:24676636).
Biological Process
Actomyosin contractile ring assembly Source: GO_Central
Glomerular visceral epithelial cell migration Source: UniProtKB
Mitotic cytokinesis Source: MGI
Positive regulation of bleb assembly Source: UniProtKB
Regulation of exit from mitosis Source: ProtInc
Septin ring assembly Source: ProtInc
Septin ring organization Source: GO_Central
Cellular Location
Cytoskeleton; Nucleus; Cell cortex; Bleb. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Involvement in disease
Focal segmental glomerulosclerosis 8 (FSGS8): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
PTM
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.

Jia, H., Gao, Z., Yu, F., Guo, H., & Li, B. (2021). Actin-binding protein anillin promotes the progression of hepatocellular carcinoma in vitro and in mice. Experimental and Therapeutic Medicine, 21(5), 1-9.

Jia, H., Yu, F., Li, B., & Gao, Z. (2021). Actin‐binding protein Anillin promotes the progression of gastric cancer in vitro and in mice. Journal of Clinical Laboratory Analysis, 35(2), e23635.

Mamoor, S. (2021). Differential expression of ANLN in cancers of the breast.

Guo, E., Mao, X., Wang, X., Guo, L., An, C., Zhang, C., ... & Miao, S. (2021). Alternatively spliced ANLN isoforms synergistically contribute to the progression of head and neck squamous cell carcinoma. Cell death & disease, 12(8), 1-14.

Dai, X., Chen, X., Hakizimana, O., & Mei, Y. (2019). Genetic interactions between ANLN and KDR are prognostic for breast cancer survival. Oncology reports, 42(6), 2255-2266.

Long, X., Zhou, W., Wang, Y., & Liu, S. (2018). Prognostic significance of ANLN in lung adenocarcinoma. Oncology letters, 16(2), 1835-1840.

Zhang, S., Nguyen, L. H., Zhou, K., Tu, H. C., Sehgal, A., Nassour, I., ... & Zhu, H. (2018). Knockdown of anillin actin binding protein blocks cytokinesis in hepatocytes and reduces liver tumor development in mice without affecting regeneration. Gastroenterology, 154(5), 1421-1434.

Idichi, T., Seki, N., Kurahara, H., Yonemori, K., Osako, Y., Arai, T., ... & Natsugoe, S. (2017). Regulation of actin-binding protein ANLN by antitumor miR-217 inhibits cancer cell aggressiveness in pancreatic ductal adenocarcinoma. Oncotarget, 8(32), 53180.

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For research use only. Not intended for any clinical use.

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