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Rabbit Anti-BTN1A1 Recombinant Antibody (CBYY-0919) (CBMAB-0922-YY)

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Basic Information

Host Animal
Rabbit
Clone
CBYY-0919
Application
FC, IF
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTN1A1/Butyrophilin Ala27-Arg242.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC0.25 µg/10^6 cells
IF(ICC)0.3-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
0.093 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Butyrophilin Subfamily 1 Member A1
Introduction
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families
Entrez Gene ID
Human696
Mouse12231
Rat306956
UniProt ID
HumanQ13410
MouseQ62556
RatF1LRV5
Alternative Names
Butyrophilin Subfamily 1 Member A1; BTN; BT; BK14H9.2 (Butyrophilin, Subfamily 1, Member A1); Butyrophilin, Subfamily 1, Member A1; BTN1;
Function
May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity).
Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity).
Biological Process
Adaptive immune response Source: Reactome
Regulation of cytokine production Source: GO_Central
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Secreted; Membrane
Topology
Extracellular: 27-242 aa
Helical: 243-269 aa
Cytoplasmic: 270-526 aa
PTM
N-glycosylated.

Rigau, M., Uldrich, A. P., & Behren, A. (2021). Targeting butyrophilins for cancer immunotherapy. Trends in immunology.

Han, L., Zhang, M., Xing, Z., Coleman, D. N., Liang, Y., Loor, J. J., & Yang, G. (2020). Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells. Journal of Animal Science and Biotechnology, 11(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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