Mouse Anti-CAPNS1 Recombinant Antibody (CBLNC-140) (CBMAB-1312-CN)

Name: Mouse Anti-CAPNS1 Recombinant Antibody (CBLNC-140)
SKU: CBMAB-1312-CN
Rating: 5 (1 reviews)

See all CAPNS1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-CAPNS1 Recombinant Antibody (CBLNC-140) (CBMAB-1312-CN) in WB

Western blot analysis of the calpain regulatory subunit after exposure to TGFβ and/or hypoxia for 8 h. ...View More

Cui, W., Zhou, J., Dehne, N., & Brüne, B. (2015). Hypoxia induces calpain activity and degrades SMAD2 to attenuate TGFβ signaling in macrophages. Cell & bioscience, 5, 1-12.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNC-140

Application

WB, IP

Immunogen

Amino acids 92-104 of Calpain reg of Calpain of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

calpain, small subunit 1

Introduction

Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. CAPNS1 is a member of the calpain small subunit family, which is essential for the stability and function of both calpain heterodimers. The proteolytic activities of CAPNS1 influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy.

Entrez Gene ID

Human	826
Cattle	281664

UniProt ID

Human	P04632
Cattle	P13135

Alternative Names

CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Function

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Biological Process

Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Regulation of macroautophagy Source: ParkinsonsUK-UCL

Cellular Location

Cytoplasm; Cell membrane. Translocates to the plasma membrane upon calcium binding.

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Zhuang, Q., Fan, M., Shen, J., Chen, Z., Xue, D., Lu, H., ... & Hou, J. (2021). Overexpression of Capns1 Predicts Poor Prognosis and Correlates with Tumor Progression in Renal Cell Carcinoma. Urologia internationalis, 1-8.

Tang, S., Yin, Q., Liu, F., & Zhang, Y. (2019). Calpain Small Subunit 1 Protein in the Prognosis of Cancer Survivors and Its Clinicopathological Correlation. BioMed research international, 2019.

MacLeod, J. A., Gao, Y., Hall, C., Muller, W. J., Gujral, T. S., & Greer, P. A. (2018). Genetic disruption of calpain-1 and calpain-2 attenuates tumorigenesis in mouse models of HER2+ breast cancer and sensitizes cancer cells to doxorubicin and lapatinib. Oncotarget, 9(70), 33382.

Grochowska, E., Borys, B., Grześkowiak, E., & Mroczkowski, S. (2017). Effect of the calpain small subunit 1 gene (CAPNS1) polymorphism on meat quality traits in sheep. Small Ruminant Research, 150, 15-21.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CAPNS1 Recombinant Antibody (CBLNC-140)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rabbit Anti-CAPNS1 Recombinant Antibody (C9695) (CAT#: CBMAB-C9695-LY)

Mouse Anti-CAPNS1 Monoclonal Antibody (1D11) (CAT#: CBMAB-0951-YC)

Mouse Anti-CAPNS1 Recombinant Antibody (CBFYC-0802) (CAT#: CBMAB-C0857-FY)

Rabbit Anti-CAPNS1 Recombinant Antibody (CBFYC-0803) (CAT#: CBMAB-C0858-FY)

Mouse Anti-CAPNS1 Recombinant Antibody (156) (CAT#: CBMAB-C3217-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry