Mouse Anti-CLDN7 Recombinant Antibody (
14F8) (V2LY-0725-LY565)

Name: Mouse Anti-CLDN7 Recombinant Antibody (14F8)
SKU: V2LY-0725-LY565
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CLDN7 Recombinant Antibody (14F8) (V2LY-0725-LY565) in IHC

IHC image of V2LY-0725-LY565 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

14F8

Application

ELISA, IHC

Immunogen

Synthesized peptide derived from human Claudin 7.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Claudin 7

Entrez Gene ID

1366

UniProt ID

O95471

Function

Plays a major role in tight junction-specific obliteration of the intercellular space.

Biological Process

Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cell adhesion Source: Ensembl
Negative regulation of protein-containing complex assembly Source: Ensembl
Positive regulation of cell motility Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Response to ethanol Source: Ensembl

Cellular Location

Cell membrane; Basolateral cell membrane; Tight junction. Co-localizes with EPCAM at the basolateral cell membrane and tight junction.

Topology

Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-160
Helical: 161-181
Cytoplasmic: 182-221

PTM

Phosphorylated.

Ding, Y., Wang, K., Xu, C., Hao, M., Li, H., & Ding, L. (2022). Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis. BMC gastroenterology, 22(1), 1-17.

Li, X., & Yang, W. (2022). IRF2‑induced Claudin‑7 suppresses cell proliferation, invasion and migration of oral squamous cell carcinoma. Experimental and Therapeutic Medicine, 23(1), 1-9.

Xu, C., Ding, Y. H., Wang, K., Hao, M., Li, H., & Ding, L. (2021). Claudin-7 deficiency promotes stemness properties in colorectal cancer through Sox9-mediated Wnt/β-catenin signalling. Journal of Translational Medicine, 19(1), 1-15.

Xing, T., Benderman, L. J., Sabu, S., Parker, J., Yang, J., Lu, Q., ... & Chen, Y. H. (2020). Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation. Cellular and molecular gastroenterology and hepatology, 9(4), 641-659.

Ji, H., Ding, X., Zhang, W., Zheng, Y., Du, H., Zheng, Y., ... & Zhong, Y. (2020). Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling. Cell Transplantation, 29, 0963689720943583.

Xu, C., Wang, K., Ding, Y. H., Li, W. J., & Ding, L. (2019). Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice. World journal of gastroenterology, 25(5), 584.

Fan, J., Tatum, R., Hoggard, J., & Chen, Y. H. (2019). Claudin-7 modulates cl− and na+ homeostasis and wnk4 expression in renal collecting duct cells. International journal of molecular sciences, 20(15), 3798.

Nguyen, N., Fernando, S. D., Biette, K. A., Hammer, J. A., Capocelli, K. E., Kitzenberg, D. A., ... & Masterson, J. C. (2018). TGF-β1 alters esophageal epithelial barrier function by attenuation of claudin-7 in eosinophilic esophagitis. Mucosal immunology, 11(2), 415-426.

Wu, Z., Shi, J., Song, Y., Zhao, J., Sun, J., Chen, X., ... & Wang, Z. (2018). Claudin-7 (CLDN7) is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, invasion and maintains mesenchymal state. Neoplasma, 65(3), 349-359.

Li, W. J., Xu, C., Wang, K., Li, T. Y., Wang, X. N., Yang, H., ... & Ding, L. (2018). Severe intestinal inflammation in the small intestine of mice induced by controllable deletion of claudin-7. Digestive diseases and sciences, 63(5), 1200-1209.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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