Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587)

Name: Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059)
SKU: V2LY-1206-LY587
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IHC

Accelerated chondrogenesis in SHIP-1 KO PαS MSCs. PαS MSC pellets differentiated in vitro for 21 days using the Mouse StemXVivo Chondrogenic Base Media and Supplement were fixed, paraffin embedded, sectioned and IHC-stained. (a and b) Sections from WT (a) and SHIP-1 KO (b) PαS MSC pellets were stained with an antibody against the early chondrogenic lineage marker Col II. Bound antibody was visualized in bright red with a NorthernLights 557-conjugated anti-sheep secondary antibody. (c and d) Sections from WT (c) and SHIP-1 KO (d) PαS MSC pellets were stained with a bright red eFluor570-conjugated antibody against the hypertrophic chondrocyte lineage marker Col X. (e and f) Sequential sections from the same WT cell pellet as in (c) and from the same SHIP-1 KO cell pellet as in (d) were subjected to H&E staining. Magnification: ×200 (a-d); ×400 (e and f). Scale bar = 50 μm. (g) Left panel, the difference in cell sizes between WT and SHIP-1 KO PαS MSCs after chondrogenic induction was quantified by using the NIH ImageJ program and indicated as ImageJ Area Units. Middle and right panels, gene expression levels of Col2a1 and Col10a1 were evaluated by quantitative real-time PCR. ...View More

So, E. Y., Sun, C., Wu, K. Q., Driesman, A., Leggett, S., Isaac, M., ... & Liang, O. D. (2020). Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development. Journal of cellular physiology, 235(2), 1425-1437.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IHC

Chondrogenic differentiation of bone marrow derived mesenchymal stem cells (BMSC). (A) Histological and Immunohistological staining of day 21 chondrogenically differentiated BMSC-pellets of the donors for Collagen type II, Thionine and Collagen type X. (B) qPCR Gene-expression analysis of chondrocyte and hypertrophy marker genes in day 21 chondrogenically differentiated BMSCs. Raw average Ct value for COL2A1 = 20.54; COL10A1 = 20.99; ALPL = 28.19. ...View More

Nossin, Y., Farrell, E., Koevoet, W. J., Somoza, R. A., Caplan, A. I., Brachvogel, B., & Van Osch, G. J. (2020). Angiogenic potential of tissue engineered cartilage from human mesenchymal stem cells is modulated by Indian hedgehog and serpin E1. Frontiers in Bioengineering and Biotechnology, 8, 327.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IHC

Representative image of collagen X stained hypertrophic callus area of an α-CGRP−/− mouse 13 days after fracture. ...View More

Niedermair, T., Straub, R. H., Brochhausen, C., & Grässel, S. (2020). Impact of the sensory and sympathetic nervous system on fracture healing in ovariectomized mice. International Journal of Molecular Sciences, 21(2), 405.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IHC

In vivo osteochondral repair in a mouse model: evaluation of chondrogenic repair (a) Histology of the tissue stained with toluidine blue (bar = 500 µm). (b) Histology of the tissue stained with Saf-O (left columns; bar = 500 µm) and the corresponding high-magnification images (right columns; bar = 100 µm). (c) Immunohistochemical analysis of repair cartilage stained for COL2 (bar = 500 µm). (d) Immunohistochemical analysis of repair tissue stained for COL10 (bar = 500 µm). bFGF, basic fibroblast growth factor; Saf-O, Safranin-O; COL2, collagen type II; COL10, collagen type X. ...View More

Okamura, G., Ebina, K., Hirao, M., Chijimatsu, R., Yonetani, Y., Etani, Y., ... & Nakata, K. (2020). Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration. International journal of molecular sciences, 22(1), 300.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IHC

Altered expression of hypertrophic differentiation markers and catabolic factors in cartilage and synovia of KAG-308-treated mice. Safranin-O staining and immunohistochemistry of Runx2, Mmp13, Col10, and Tnf in (a) articular cartilage and (b) intercondylar synovium of the KAG-308-treated mice 8 wks after OA induction (eight mice per group). Inset boxes in safranin-O staining indicate enlarged images of immunohistochemistry. Scale bar, 50 µm. Right graphs indicate mean pixel intensity per µm2 or positive cell rates in the immunohistochemistry. Symbols represent individual mice; long and short bars show the mean and SD, respectively. *P < 0.05, **P < 0.005, ***P < 0.0005 by Welch's t test, n = 8 mice per group. ...View More

Murahashi, Y., Yano, F., Chijimatsu, R., Nakamoto, H., Maenohara, Y., Amakawa, M., ... & Saito, T. (2019). Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion. Scientific reports, 9(1), 20329.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COL10A1 Recombinant Antibody (CBCNC-059) (V2LY-1206-LY587) in IF

Immunofluorescence images illustrating deep and surface sections. Staining of collagen I (red), collagen II (green), aggrecan (green) and collagen X (green) was performed on constructs from all groups on day 28 (as indicated; DAPI nuclei staining in light blue); (A,E,I): GelMA-ALG; (B,F,J): GelMA/HAMA-ALG; (C,G,K): GelMA-ALG/HAP and (D,H,L): GelMA/HAMA-ALG/HAP. Images were taken from the deep hydrogel zone near to the ALG or ALG/HAP scaffold, with surface images inset; scale bar 50 µm. ...View More

Bartnikowski, M., Akkineni, A. R., Gelinsky, M., Woodruff, M. A., & Klein, T. J. (2016). A hydrogel model incorporating 3D-plotted hydroxyapatite for osteochondral tissue engineering. Materials, 9(4), 285.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBCNC-059

Application

ELISA, IF, IHC, WB

Immunogen

Human COL10A1.

Host Species

Mouse

Specificity

Dog, Guinea pig, Human, Mouse, Pig, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC-P	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type X Alpha 1 Chain

Entrez Gene ID

Human	1300
Mouse	12813
Rat	25681
Dog	100856711
Guinea pig	106026924
Pig	448809

UniProt ID

Human	Q03692
Mouse	Q05306
Rat	A0A0G2K7A5
Dog	J9P1I7
Guinea pig	H0VEK5
Pig	Q9N178

Function

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

Biological Process

Collagen fibril organization Source: Reactome
Extracellular matrix organization Source: GO_Central
Skeletal system development Source: ProtInc

Cellular Location

Extracellular matrix

Involvement in disease

Schmid type metaphyseal chondrodysplasia (SMCD):
Dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Lv, D., Chen, D., Wang, Z., Cui, Z., Ma, J. H., Ji, S., ... & Tang, S. (2022). COL10A1 is a novel factor in the development of choroidal neovascularization. Microvascular Research, 139, 104239.

Chen, S., Wei, Y., Liu, H., Gong, Y., Zhou, Y., Yang, H., & Tang, L. (2021). Analysis of Collagen type X alpha 1 (COL10A1) expression and prognostic significance in gastric cancer based on bioinformatics. Bioengineered, 12(1), 127-137.

Wu, H., Wang, S., Li, G., Yao, Y., Wang, N., Sun, X., ... & Xu, C. (2021). Characterization of a novel COL10A1 variant associated with Schmid‐type metaphyseal chondrodysplasia and a literature review. Molecular genetics & genomic medicine, 9(5), e1668.

Yang, W., Wu, X., & Zhou, F. (2021). Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 27, e928919-1.

Aktas, S. H., Taskin-Tok, T., Al-Khafaji, K., & Akın-Balı, D. F. (2021). A detailed understanding of the COL10A1 and SOX9 genes interaction based on potentially damaging mutations in gastric cancer using computational techniques. Journal of Biomolecular Structure and Dynamics, 1-12.

Zhang, M., Chen, H., Wang, M., Bai, F., & Wu, K. (2020). Bioinformatics analysis of prognostic significance of COL10A1 in breast cancer. Bioscience Reports, 40(2).

Zhang, X., Liang, H., Liu, W., Li, X., Zhang, W., & Shang, X. (2019). A novel sequence variant in COL10A1 causing spondylometaphyseal dysplasia accompanied with coxa valga: A case report. Medicine, 98(30).

Huang, H., Li, T., Ye, G., Zhao, L., Zhang, Z., Mo, D., ... & Liu, H. (2018). High expression of COL10A1 is associated with poor prognosis in colorectal cancer. OncoTargets and therapy, 11, 1571.

Zhang, C., Liu, J., Iqbal, F., Lu, Y., Mustafa, S., Bukhari, F., ... & Xu, S. (2018). A missense point mutation in COL10A1 identified with whole-genome deep sequencing in a 7-generation Pakistan dwarf family. Heredity, 120(1), 83-89.

ul Ain, N., Makitie, O., & Naz, S. (2018). Autosomal recessive chondrodysplasia with severe short stature caused by a biallelic COL10A1 variant. Journal of Medical Genetics, 55(6), 403-407.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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