Mouse Anti-COL5A1 Recombinant Antibody (CBFYC-0392) (V2LY-1206-LY682)

Name: Mouse Anti-COL5A1 Recombinant Antibody (CBFYC-0392)
SKU: V2LY-1206-LY682
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-COL5A1 Recombinant Antibody (CBFYC-0392) (V2LY-1206-LY682) in IHC

Immunohistochemical analysis of candidate markers. IHC was carried out on tongue cancer samples (A) with antibodies to HBB (a, b,c, d) and COL5A1 (e, f, g, h). ...View More

Suresh, A., Vannan, M., Kumaran, D., Gümüs, Z. H., Sivadas, P., Murugaian, E. E., ... & Kuriakose, M. A. (2012). Resistance/response molecular signature for oral tongue squamous cell carcinoma. Disease markers, 32(1), 51-64.

Distributed under Open Access license CC BY 3.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0392

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 251-450 mapping within an extracellular domain of Collagen α1 Type V of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type V Alpha 1 Chain

Entrez Gene ID

1289

UniProt ID

P20908

Function

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Biological Process

Blood vessel development Source: Ensembl
Cell adhesion Source: UniProtKB
Cell migration Source: UniProtKB
Collagen biosynthetic process Source: UniProtKB
Collagen fibril organization Source: UniProtKB
Extracellular matrix organization Source: GO_Central
Eye morphogenesis Source: UniProtKB
Heart morphogenesis Source: Ensembl
Integrin biosynthetic process Source: UniProtKB
Negative regulation of endodermal cell differentiation Source: UniProtKB
Regulation of cellular component organization Source: Ensembl
Skin development Source: UniProtKB
Supramolecular fiber organization Source: UniProtKB
Tendon development Source: Ensembl
Wound healing, spreading of epidermal cells Source: UniProtKB

Cellular Location

Extracellular matrix

Involvement in disease

Ehlers-Danlos syndrome, classic type, 1 (EDSCL1):
A form of Ehlers-Danlos syndrome, a group of connective tissue disorders characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. The main features of classic Ehlers-Danlos syndrome are joint hypermobility and dislocation, and fragile, bruisable skin. EDSCL1 inheritance is autosomal dominant.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.

Zhu, H., Hu, X., Feng, S., Jian, Z., Xu, X., Gu, L., & Xiong, X. (2022). The Hypoxia-Related Gene COL5A1 Is a Prognostic and Immunological Biomarker for Multiple Human Tumors. Oxidative Medicine and Cellular Longevity, 2022.

Tsai, H. F., Chang, Y. C., Li, C. H., Chan, M. H., Chen, C. L., Tsai, W. C., & Hsiao, M. (2021). Type V collagen alpha 1 chain promotes the malignancy of glioblastoma through PPRC1-ESM1 axis activation and extracellular matrix remodeling. Cell death discovery, 7(1), 1-12.

Machol, K., Polak, U., Weisz-Hubshman, M., Song, I. W., Chen, S., Jiang, M. M., ... & Lee, B. H. (2021). Molecular alterations due to Col5a1 haploinsufficiency in a mouse model of classic Ehlers–Danlos syndrome. Human molecular genetics.

Zhang, J., Zhang, J., Wang, F., Xu, X., Li, X., Guan, W., ... & Xu, G. (2021). Overexpressed COL5A1 is correlated with tumor progression, paclitaxel resistance, and tumor‐infiltrating immune cells in ovarian cancer. Journal of Cellular Physiology, 236(10), 6907-6919.

Zheng, J., Pang, C. H., Du, W., Wang, L., Sun, L. G., & Xing, Z. Y. (2020). An allele of rs619586 polymorphism in MALAT1 alters the invasiveness of meningioma via modulating the expression of collagen type V alpha (COL5A1). Journal of Cellular and Molecular Medicine, 24(17), 10223-10232.

Wu, M., Sun, Q., Mo, C. H., Pang, J. S., Hou, J. Y., Pang, L. L., ... & Feng, Z. B. (2019). Prospective molecular mechanism of COL5A1 in breast cancer based on a microarray, RNA sequencing and immunohistochemistry. Oncology Reports, 42(1), 151-175.

Lin, Z., Zeng, J., & Wang, X. (2019). Compound phenotype of osteogenesis imperfecta and Ehlers–Danlos syndrome caused by combined mutations in COL1A1 and COL5A1. Bioscience Reports, 39(7).

Pabalan, N., Tharabenjasin, P., Phababpha, S., & Jarjanazi, H. (2018). Association of COL5A1 gene polymorphisms and risk of tendon-ligament injuries among Caucasians: a meta-analysis. Sports medicine-open, 4(1), 1-11.

Longo, U. G., Margiotti, K., Petrillo, S., Rizzello, G., Fusilli, C., Maffulli, N., ... & Denaro, V. (2018). Genetics of rotator cuff tears: no association of col5a1 gene in a case-control study. BMC Medical Genetics, 19(1), 1-5.

Lv, Z. T., Gao, S. T., Cheng, P., Liang, S., Yu, S. Y., Yang, Q., & Chen, A. M. (2018). Association between polymorphism rs12722 in COL5A1 and musculoskeletal soft tissue injuries: a systematic review and meta-analysis. Oncotarget, 9(20), 15365.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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