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Mouse Anti-CSNK1d Recombinant Antibody (CBFYH-0116) (CBMAB-H0977-FY)

This product is mouse antibody that recognizes CSNK1d. The antibody CBFYH-0116 can be used for immunoassay techniques such as: WB, ICC, IHC-P, IHC-Fr, ELISA.
See all CSNK1d antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0116
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% glycerol
Preservative
0.02% Sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
casein kinase 1, delta
Introduction
CSNK1D (Casein Kinase 1 Delta) is a Protein Coding gene. Diseases associated with CSNK1D include Advanced Sleep Phase Syndrome, Familial, 2 and Advanced Sleep Phase Syndrome 2. Among its related pathways are Organelle biogenesis and maintenance and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CSNK1E.
Entrez Gene ID
1453
UniProt ID
P48730
Alternative Names
Casein Kinase 1 Delta; Tau-Protein Kinase CSNK1D; EC 2.7.11.1; CKI-Delta; HCKID; CKId; Casein Kinase I Isoform Delta; Casein Kinase 1, Delta
Function
Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate.
Biological Process
Ciliary basal body-plasma membrane docking Source: Reactome
Circadian regulation of gene expression Source: UniProtKB
COPII vesicle coating Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Golgi organization Source: SYSCILIA_CCNET
Microtubule nucleation Source: SYSCILIA_CCNET
Non-motile cilium assembly Source: SYSCILIA_CCNET
Peptidyl-serine phosphorylation Source: ParkinsonsUK-UCL
Positive regulation of canonical Wnt signaling pathway Source: BHF-UCL
Positive regulation of non-canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Positive regulation of protein phosphorylation Source: BHF-UCL
Positive regulation of Wnt-mediated midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Protein localization to centrosome Source: SYSCILIA_CCNET
Protein localization to cilium Source: SYSCILIA_CCNET
Protein localization to Golgi apparatus Source: SYSCILIA_CCNET
Protein phosphorylation Source: UniProtKB
Regulation of circadian rhythm Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Spindle assembly Source: UniProtKB
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Cytoplasm; Nucleus; Golgi apparatus; Cell membrane; Centrosome; Spindle; Perinuclear region. Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity.
Involvement in disease
Advanced sleep phase syndrome, familial, 2 (FASPS2):
A disorder characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
PTM
Autophosphorylated on serine and threonine residues; this autophosphorylation represses activity. Reactivated by phosphatase-mediated dephosphorylation. May be dephosphorylated by PP1.

Peer, E., Aichberger, S. K., Vilotic, F., Gruber, W., Parigger, T., Grund-Gröschke, S., ... & Aberger, F. (2021). Casein Kinase 1D Encodes a Novel Drug Target in Hedgehog—GLI-Driven Cancers and Tumor-Initiating Cells Resistant to SMO Inhibition. Cancers, 13(16), 4227.

Vena, F., Bayle, S., Nieto, A., Quereda, V., Aceti, M., Frydman, S. M., ... & Duckett, D. (2020). Targeting casein kinase 1 delta sensitizes pancreatic and bladder cancer cells to gemcitabine treatment by upregulating deoxycytidine kinase. Molecular cancer therapeutics, 19(8), 1623-1635.

Xu, P., Ianes, C., Gärtner, F., Liu, C., Burster, T., Bakulev, V., ... & Bischof, J. (2019). Structure, regulation, and (patho-) physiological functions of the stress-induced protein kinase CK1 delta (CSNK1D). Gene, 715, 144005.

Guo, G., Wang, K., Hu, S. S., Tian, T., Liu, P., Mori, T., ... & Qin, X. (2019). Autokinase activity of casein kinase 1 δ/ε governs the period of mammalian circadian rhythms. Journal of biological rhythms, 34(5), 482-496.

Bar, I., Merhi, A., Larbanoix, L., Constant, M., Haussy, S., Laurent, S., ... & Delrée, P. (2018). Silencing of casein kinase 1 delta reduces migration and metastasis of triple negative breast cancer cells. Oncotarget, 9(56), 30821.

Sciabola, S., Benedetti, P., D’Arrigo, G., Torella, R., Baroni, M., Cruciani, G., & Spyrakis, F. (2018). Discovering New Casein Kinase 1d Inhibitors with an Innovative Molecular Dynamics Enabled Virtual Screening Workflow. ACS medicinal chemistry letters, 10(4), 487-492.

Eng, G. W. L., & Virshup, D. M. (2017). Site-specific phosphorylation of casein kinase 1 δ (CK1δ) regulates its activity towards the circadian regulator PER2. PLoS One, 12(5), e0177834.

Greer, Y. E., Gao, B., Yang, Y., Nussenzweig, A., & Rubin, J. S. (2017). Lack of casein kinase 1 delta promotes genomic instability-the accumulation of dna damage and down-regulation of checkpoint kinase 1. PloS one, 12(1), e0170903.

Morgenstern, Y., Das Adhikari, U., Ayyash, M., Elyada, E., Tóth, B., Moor, A., ... & Ben‐Neriah, Y. (2017). Casein kinase 1‐epsilon or 1‐delta required for Wnt‐mediated intestinal stem cell maintenance. The EMBO journal, 36(20), 3046-3061.

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For research use only. Not intended for any clinical use.

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