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Mouse Anti-CTH Recombinant Antibody (CBWJC-3329) (V2LY-0125-LY261)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Dog
Clone
CBWJC-3329
Antibody Isotype
IgG1
Application
WB, IHC, IF

Basic Information

Immunogen
Gamma cystathionase Fusion Protein (74-406 aa).
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:2,000-1:6,000
IHC-P1:200-1:800
IF(ICC)1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Glycerol & PBS
Preservative
Sodium Azide
Concentration
1 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cystathionine Gamma-Lyase
Entrez Gene ID
Human1491
Mouse107869
Rat24962
UniProt ID
HumanP32929
MouseQ8VCN5
RatP18757
Function
Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
Biological Process
Cellular response to leukemia inhibitory factor Source: Ensembl
Cysteine biosynthetic process Source: UniProtKB
Cysteine biosynthetic process via cystathionine Source: BHF-UCL
Cysteine metabolic process Source: ProtInc
Endoplasmic reticulum unfolded protein response Source: UniProtKB
Hydrogen sulfide biosynthetic process Source: UniProtKB
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of aortic smooth muscle cell differentiation Source: BHF-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
Positive regulation of NF-kappaB transcription factor activity Source: Ensembl
Protein homotetramerization Source: UniProtKB
Protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine Source: UniProtKB
Protein sulfhydration Source: UniProtKB
Sulfur amino acid catabolic process Source: Reactome
Transsulfuration Source: BHF-UCL
Cellular Location
Cytoplasm
Involvement in disease
Cystathioninuria (CSTNU):
Autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
More Infomation

Lancien, M., Gueno, L., Salle, S., Merieau, E., Beriou, G., Nguyen, T. H., ... & Louvet, C. (2021). Cystathionine‐gamma‐lyase overexpression in T cells enhances antitumor effect independently of cysteine autonomy. Cancer Science, 112(5), 1723.

Xu, S., Pan, J., Cheng, X., Zheng, J., Wang, X., Guan, H., ... & Zhang, L. (2020). Diallyl trisulfide, a H2S donor, inhibits cell growth of human papillary thyroid carcinoma KTC‐1 cells through a positive feedback loop between H2S and cystathionine‐gamma‐lyase. Phytotherapy Research, 34(5), 1154-1165.

Chen, L., Lu, C., & Hua, Y. (2020). Cystathionine gamma‐lyase aggravates periodontal damage in traumatic occlusion mouse models. Journal of periodontal research, 55(5), 667-675.

Akahoshi, N., Handa, H., Takemoto, R., Kamata, S., Yoshida, M., Onaka, T., & Ishii, I. (2019). Preeclampsia-like features and partial lactation failure in mice lacking cystathionine γ-lyase—an animal model of cystathioninuria. International Journal of Molecular Sciences, 20(14), 3507.

Wang, Y. H., Huang, J. T., Chen, W. L., Wang, R. H., Kao, M. C., Pan, Y. R., ... & Wang, L. H. (2019). Dysregulation of cystathionine γ‐lyase promotes prostate cancer progression and metastasis. EMBO reports, 20(10), e45986.

Lechuga, T. J., Qi, Q. R., Kim, T., Magness, R. R., & Chen, D. B. (2019). E2β stimulates ovine uterine artery endothelial cell H2S production in vitro by estrogen receptor-dependent upregulation of cystathionine β-synthase and cystathionine γ-lyase expression. Biology of reproduction, 100(2), 514-522.

Szijártó, I. A., Markó, L., Filipovic, M. R., Miljkovic, J. L., Tabeling, C., Tsvetkov, D., ... & Gollasch, M. (2018). Cystathionine γ-Lyase–Produced hydrogen sulfide controls endothelial no bioavailability and blood pressure. Hypertension, 71(6), 1210-1217.

Gupta, V., Kapopara, P. R., Khan, A. A., Arige, V., Subramanian, L., Sonawane, P. J., ... & Mahapatra, N. R. (2017). Functional promoter polymorphisms direct the expression of cystathionine gamma-lyase gene in mouse models of essential hypertension. Journal of Molecular and Cellular Cardiology, 102, 61-73.

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For research use only. Not intended for any clinical use.

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